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Article

Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway

  • Authors:
    • Juan Li
    • Fuyu Qiu
    • Lu Yu
    • Yanbo Zhao
    • Guosheng Fu
    • Binquan Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, Zhejiang, P.R. China
  • Pages: 598-602
    |
    Published online on: November 12, 2012
       https://doi.org/10.3892/mmr.2012.1180
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Abstract

We previously demonstrated that thymosin β4 (Tβ4) regulates a variety of endothelial progenitor cell (EPC) functions, including cell migration, proliferation, survival and angiogenesis. However, the effect of Tβ4 on the senescence of circulating EPCs remains unclear. In the present study, the effect of Tβ4 on EPC senescence and the signal transduction pathways involved in this process was investigated. Circulating EPCs isolated from healthy volunteers were cultured in the absence or presence of Tβ4 and various signal cascade inhibitors. Tβ4 inhibited EPC senescence in a concentration‑dependent manner. In addition, Tβ4 increased telomerase activity and expression of telomerase reverse transcriptase mRNA in EPCs. Tβ4 also regulated the expression of p21, p27 and cyclin D1. The effects of Tβ4 on EPC senescence were eliminated by the phosphoinositide 3'‑kinase (PI3K) inhibitor, wortmannin and the endothelial nitric oxide synthase inhibitor, L‑nitroarginine methyl ester hydrochloride (L‑NAME). In conclusion, the inhibitory effect on EPC senescence mediated by Tβ4 may be attributed, at least in part, to activation of the PI3K‑Akt‑eNOS signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Qiu F, Yu L, Zhao Y, Fu G and Zhou B: Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway. Mol Med Rep 7: 598-602, 2013.
APA
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., & Zhou, B. (2013). Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway. Molecular Medicine Reports, 7, 598-602. https://doi.org/10.3892/mmr.2012.1180
MLA
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., Zhou, B."Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway". Molecular Medicine Reports 7.2 (2013): 598-602.
Chicago
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., Zhou, B."Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway". Molecular Medicine Reports 7, no. 2 (2013): 598-602. https://doi.org/10.3892/mmr.2012.1180
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Qiu F, Yu L, Zhao Y, Fu G and Zhou B: Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway. Mol Med Rep 7: 598-602, 2013.
APA
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., & Zhou, B. (2013). Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway. Molecular Medicine Reports, 7, 598-602. https://doi.org/10.3892/mmr.2012.1180
MLA
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., Zhou, B."Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway". Molecular Medicine Reports 7.2 (2013): 598-602.
Chicago
Li, J., Qiu, F., Yu, L., Zhao, Y., Fu, G., Zhou, B."Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/Akt/eNOS signal transduction pathway". Molecular Medicine Reports 7, no. 2 (2013): 598-602. https://doi.org/10.3892/mmr.2012.1180
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