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Article

Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China

  • Authors:
    • Hong-Mei Liu
    • Peng Cheng
    • Xiaodan Huang
    • Yu-Hua Dai
    • Hai-Fang Wang
    • Li-Juan Liu
    • Yu-Qiang Zhao
    • Huai-Wei Wang
    • Mao-Qing Gong
  • View Affiliations / Copyright

    Affiliations: Department of Insects, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, Shandong 272033, P.R. China
  • Pages: 525-530
    |
    Published online on: November 13, 2012
       https://doi.org/10.3892/mmr.2012.1184
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Abstract

The present study aimed to investigate deltamethrin resistance in Culex pipiens pallens (C. pipiens pallens) mosquitoes and its correlation with knockdown resistance (kdr) mutations. In addition, mosquito‑resistance testing methods were analyzed. Using specific primers in polymerase chain reaction (PCR) and allele-specific (AS)-PCR, kdr gene sequences isolated from wild C. pipiens pallens mosquitoes were sequenced. Linear regression analysis was used to determine the correlation between the mutations and deltamethrin resistance. A kdr allelic gene was cloned and sequenced. Analysis of the DNA sequences revealed the presence of two point mutations at the L1014 residue in the IIS6 transmembrane segment of the voltage‑gated sodium channel (VGSC): L1014F, TTA→TTT, replacing a leucine (L) with a phenylalanine (F); L1014S, TTA→TCA, replacing leucine (L) with serine (S). Two alternative kdr-like mutations, L1014F and L1014S, were identified to be positively correlated with the deltamethrin-resistant phenotype. In addition a novel mutation, TCT, was identified in the VGSC of C. pipiens pallens. PCR and AS-PCR yielded consistent results with respect to mosquito resistance. However, the detection rate of PCR was higher than that of AS-PCR. Further studies are required to determine the specific resistance mechanism. PCR and AS-PCR demonstrated suitability for mosquito resistance field tests, however, the former method may be superior to the latter.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Cheng P, Huang X, Dai Y, Wang H, Liu L, Zhao Y, Wang H and Gong M: Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Mol Med Rep 7: 525-530, 2013.
APA
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L. ... Gong, M. (2013). Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Molecular Medicine Reports, 7, 525-530. https://doi.org/10.3892/mmr.2012.1184
MLA
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L., Zhao, Y., Wang, H., Gong, M."Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China". Molecular Medicine Reports 7.2 (2013): 525-530.
Chicago
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L., Zhao, Y., Wang, H., Gong, M."Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China". Molecular Medicine Reports 7, no. 2 (2013): 525-530. https://doi.org/10.3892/mmr.2012.1184
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Cheng P, Huang X, Dai Y, Wang H, Liu L, Zhao Y, Wang H and Gong M: Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Mol Med Rep 7: 525-530, 2013.
APA
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L. ... Gong, M. (2013). Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Molecular Medicine Reports, 7, 525-530. https://doi.org/10.3892/mmr.2012.1184
MLA
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L., Zhao, Y., Wang, H., Gong, M."Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China". Molecular Medicine Reports 7.2 (2013): 525-530.
Chicago
Liu, H., Cheng, P., Huang, X., Dai, Y., Wang, H., Liu, L., Zhao, Y., Wang, H., Gong, M."Identification of TCT, a novel knockdown resistance allele mutation and analysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China". Molecular Medicine Reports 7, no. 2 (2013): 525-530. https://doi.org/10.3892/mmr.2012.1184
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