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Article

Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun

  • Authors:
    • Yanping Ma
    • Zhihua Li
    • Yihua Wang
    • Jing Feng
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
  • Pages: 481-484
    |
    Published online on: November 21, 2012
       https://doi.org/10.3892/mmr.2012.1194
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Abstract

The aim of this study was to investigate the mechanism of the apoptotic effect of brucine on human multiple myeloma (MM) cells. U266 cells (5x104) were plated in the presence or absence of brucine (0, 0.05, 0.1, 0.2 and 0.4 mg/ml) in 96‑well culture plates for 24‑72 h. The anti-proliferative response to brucine was assessed by MTT assay. Analysis of the cell cycle of U266 cells treated with or without brucine was performed using flow cytometry. The expression change of c-Jun following treatment with brucine or brucine plus the c-Jun N-terminal kinase (JNK)-specific inhibitor SP600125 was detected using RT-PCR. Brucine appeared to have an effect on apoptosis in a dose- and time‑dependent manner. Cell cycle analysis using flow cytometry revealed the accumulation of cells at the sub-G0/G1 phase. The apoptotic rates were 4.137, 10.55, 12.31, 27.67 and 29.67% (0, 0.05, 0.1, 0.2, 0.4 mg/ml brucine, respectively; P<0.01). The gray scale values were 0.7961±0.007 and 0.4683±0.003 (mRNA expression of c-Jun of U266 cells with or without SP600125, respectively). Concentrations of ≤0.4 mg/ml brucine induced apoptosis in U266 cells. Thus, brucine‑induced apoptosis in U266 cells occurs via the JNK signaling pathway and phosphorylation of c-Jun.
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View References

1 

Wei ZL and Wang XH: The development of NF-κB in the multiple myeloma. Med Res Nov. 36:98–101. 2007.

2 

Spisek R, Charalambous A, Mazumder A, et al: Bortezomib enhances dendritic cell (DC)-mediated induction of immunity to human myeloma via exposure of cell surface heat shock protein 90 on dying tumor cells: therapeutic implications. Blood. 109:4839–4845. 2007. View Article : Google Scholar

3 

Rao PS, Ramanadham M and Prasad MN: Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line - RPMI 8226. Food Chem Toxicol. 47:283–288. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Deng X, Yin F, Lu X, et al: The apoptotic effect of brucine from the seed of Strychnos nux-vomica on human hepatoma cells is mediated via Bcl-2 and Ca2+ involved mitochondrial pathway. Toxicol Sci. 91:59–69. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Li ZH, Ma YP, Wang YH and Feng J: Apoptosis of U266 cells induced by brucine through death-receptor pathway. J Leuk Lymphoma. 19:724–731. 2010.

6 

Jiao JX and Gao WJ: The research progress of the mechanism on the cellular signal transmission. Chin J Gerontol. 30:853–856. 2010.(In Chinese).

7 

Wen ZH and Wang XH: Apoptosis and multiple myeloma. North China Coal Med Coll. 8:778–781. 2006.

8 

Liang XM and Yang KD: Caspase, JNK/SAPK, P38 MAPK and apoptosis. Foreign Med Sci (section hygiene). 35:5–10. 2008.

9 

Wan KF, Chan SL, Sukumaran SK, et al: Chelerythrine induces apoptosis through a Bax/Bak-independent mitochondrial mechanism. J Biol Chem. 283:8423–8433. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Du L, Wang FY, Zhang L, et al: Advance in the research of JNK dependent apoptosis. China Trop Med. 18:841–844. 2008.(In Chinese).

11 

Xiao Y, Yang FQ, Li SP, et al: Furanodiene induces G2/M cell cycle arrest and apoptosis through MAPK signaling and mitochondria-caspase pathway in human hepatocellular carcinoma cells. Cancer Biol Ther. 6:1044–1050. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Hu Z, Tao YG, Yang LF, et al: Effect of JIP on the proliferation and apoptosis of nasopharyngeal carcinoma cells through interaction with JNK mediated pathway. Prog Biochem Biophys. 30:579–585. 2003.PubMed/NCBI

13 

Papa S, Zazzeroni F, Pham CG, et al: Linking JNK signaling to NF-kappaB: a key to survival. J Cell Sci. 117:5197–5208. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Murakami Y, Aizu-Yokota E, Sonoda Y, et al: Suppression of endoplasmic reticulum stress induced caspase activation and cell death by the over expression of Bcl-xl or Bcl-2. J Biochem. 141:401–410. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Peng J, Mao XO, Stevenson FF, et al: The herbicide paraquat induces dopaminergic nigral apoptosis through sustained activation of the JNK pathway. J Biol Chem. 279:32626–32632. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Largo C, Alvarez S, Saez B, et al: Identification of overexpressed genes in frequently gained/amplified chromosome regions in multiple myeloma. Hematologica. 91:184–191. 2004.PubMed/NCBI

17 

Lin HH, Chen JH, Kuo WH, et al: Chemopreventive properties of Hibiscus sabdariffa L on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation. Chem Biol Interac. 165:59–75. 2007.

18 

Gong X, Wang M, Tashiro S, et al: Involvement of JNK-initiated p53 acccumulation and phosphorylation of p53 in pseudolaric acid B induced cell death. Exp Mol Med. 38:428–434. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Junttila MR, Li SP and Westermarck J: Phosphatase-mediated crosstalk between MAPK signaling pathways in the regulation of cell survival. FASEB J. 22:954–965. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Matsumoto N, Imamura R and Suda T: Caspase-8 and JNK dependent AP-1 activation is required for Fas ligand-induced IL-8 production. FEBS J. 274:2376–2384. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Li Z, Wang Y and Feng J: Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun . Mol Med Rep 7: 481-484, 2013.
APA
Ma, Y., Li, Z., Wang, Y., & Feng, J. (2013). Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun . Molecular Medicine Reports, 7, 481-484. https://doi.org/10.3892/mmr.2012.1194
MLA
Ma, Y., Li, Z., Wang, Y., Feng, J."Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun ". Molecular Medicine Reports 7.2 (2013): 481-484.
Chicago
Ma, Y., Li, Z., Wang, Y., Feng, J."Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun ". Molecular Medicine Reports 7, no. 2 (2013): 481-484. https://doi.org/10.3892/mmr.2012.1194
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Li Z, Wang Y and Feng J: Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun . Mol Med Rep 7: 481-484, 2013.
APA
Ma, Y., Li, Z., Wang, Y., & Feng, J. (2013). Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun . Molecular Medicine Reports, 7, 481-484. https://doi.org/10.3892/mmr.2012.1194
MLA
Ma, Y., Li, Z., Wang, Y., Feng, J."Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun ". Molecular Medicine Reports 7.2 (2013): 481-484.
Chicago
Ma, Y., Li, Z., Wang, Y., Feng, J."Brucine induces the apoptosis of U266 multiple myeloma cells by phosphorylation of c-Jun ". Molecular Medicine Reports 7, no. 2 (2013): 481-484. https://doi.org/10.3892/mmr.2012.1194
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