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Article

I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells

  • Authors:
    • Jin-Nyoung Ho
    • Woojin Jun
    • Ryowon Choue
    • Jeongmin Lee
  • View Affiliations / Copyright

    Affiliations: Department of Medical Nutrition, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea, Human Ecology Research Institute, Department of Food and Nutrition, Chonnam National University, Gwangju 500-757, Republic of Korea, Research Institute of Medical Nutrition, Kyung Hee University, Seoul 130-701, Republic of Korea
  • Pages: 384-388
    |
    Published online on: November 23, 2012
       https://doi.org/10.3892/mmr.2012.1198
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Abstract

Indole-3-carbinol (I3C) and indole[3,2-b] carbazole (ICZ) are major bioactive food components in cruciferous vegetables. Although previous studies have demonstrated the anticancer activity of I3C and ICZ in various types of cancer cells, the manner in which indole compounds regulate migration or related epithelial-to-mesenchymal transitions (EMT) has yet to be determined. In this study, we investigated the effects of I3C and ICZ on migration using breast cancer cells (MCF-7 and MDA-MB231). Pre‑treatment with I3C and ICZ significantly inhibited the migration of breast cancer cells without cytotoxicity, as measured by monolayer scratch assay. In addition, I3C and ICZ decreased vimentin (a mesenchymal marker) and focal adhesion kinase (FAK) mRNA expression, while increasing E-cadherin (an epithelial marker) expression. Matrix metalloproteinase (MMP)-2 and -9 activity was also reduced by I3C and ICZ. Taken together, we propose that I3C and ICZ pre‑treatment inhibits the migration of breast cancer cells through suppression of the EMT process and reduced MMP activity by repressing FAK expression. Our findings suggested that I3C and ICZ are potential compounds for inhibition of breast cancer cell migration.
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1 

Jemal A, Center MM, DeSantis C and Ward EM: Global patterns of cancer incidence and mortality rates and trends. Cancer Epidemiol Biomarkers Prev. 19:1893–1907. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Luo M and Guan JL: Focal adhesion kinase: a prominent determinant in breast cancer initiation, progression and metastasis. Cancer Lett. 289:127–139. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Hsu YL, Wu LY, Hou MF, Tsai EM, Lee JN, Liang HL, Jong YJ, Hung CH and Kuo PL: Glabridin, an isoflavan from licorice root, inhibits migration, invasion and angiogenesis of MDA-MB-231 human breast adenocarcinoma cells by inhibiting focal adhesion kinase/Rho signaling pathway. Mol Nutr Food Res. 55:318–327. 2011. View Article : Google Scholar

4 

Park SK, Kim Y, Kang D, Jung EJ and Yoo KY: Risk factors and control strategies for the rapidly rising rate of breast cancer in Korea. J Breast Cancer. 14:79–87. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Hay ED: The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it. Dev Dyn. 233:706–720. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Sarkar FH, Li Y, Wang Z and Kong D: Pancreatic cancer stem cells and EMT in drug resistance and metastasis. Minerva Chir. 64:489–500. 2009.PubMed/NCBI

8 

Hardy KM, Booth BW, Hendrix MJ, Salomon DS and Strizzi L: ErbB/EGF signaling and EMT in mammary development and breast cancer. J Mammary Gland Biol Neoplasia. 15:191–199. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Foroni C, Massimo B, Generali D and Damia G: Epithelial-mesenchymal transition and breast cancer: role, molecular mechanisms and clinical impact. Cancer Treat Rev. 38:689–697. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Stetler-Stevenson WG, Aznavoorian S and Liotta LA: Tumor cell interactions with the extracellular matrix during invasion and metastasis. Annu Rev Cell Biol. 9:541–573. 1993. View Article : Google Scholar : PubMed/NCBI

11 

Nabeshima K, Inoue T, Shimao Y and Sameshima T: Matrix metalloproteinases in tumor invasion: role for cell migration. Pathol Int. 52:255–264. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Zhao J and Guan JL: Signal transduction by focal adhesion kinase in cancer. Cancer Metastasis Rev. 28:35–49. 2009. View Article : Google Scholar : PubMed/NCBI

13 

van Nimwegen MJ and van de Water B: Focal adhesion kinase: a potential target in cancer therapy. Biochem Pharmacol. 73:597–609. 2007.PubMed/NCBI

14 

Cortes-Reynosa P, Robledo T, Macias-Silva M, Wu SV and Salazar EP: Src kinase regulates metalloproteinase-9 secretion induced by type IV collagen in MCF-7 human breast cancer cells. Matrix Biol. 27:220–231. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Chen PN, Chu SC, Kuo WH, Chou MY, Lin JK and Hsieh YS: Epigallocatechin-3 gallate inhibits invasion, epithelial-mesenchymal transition, and tumor growth in oral cancer cells. Agric Food Chem. 59:3836–3844. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Vergara D, Valente CM, Tinelli A, Siciliano C, Lorusso V, Acierno R, Giovinazzo G, Santino A, Storelli C and Maffia M: Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells. Cancer Lett. 310:1–8. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Meng Q, Goldberg ID, Rosen EM and Fan S: Inhibitory effects of Indole-3-carbinol on invasion and migration in human breast cancer cells. Breast Cancer Res Treat. 63:147–152. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Anderton MJ, Manson MM, Verschoyle RD, Gescher A, Lamb JH, Farmer PB, Steward WP and Williams ML: Pharmacokinetics and tissue disposition of indole-3-carbinol and its acid condensation products after oral administration to mice. Clin Cancer Res. 10:5233–5241. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Liu H, Wormke M, Safe SH and Bjeldanes LF: Indolo[3,2-b]carbazole: a dietary-derived factor that exhibits both antiestrogenic and estrogenic activity. J Natl Cancer Inst. 86:1758–1765. 1994.

20 

Chen YH, Dai HJ and Chang HP: Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages. Planta Med. 69:696–700. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Lauffenburger DA and Horwitz AF: Cell migration: a physically integrated molecular process. Cell. 84:359–369. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT and Horwitz AR: Cell migration: integrating signals from front to back. Science. 302:1704–1709. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Guarino M, Rubino B and Ballabio G: The role of epithelial-mesenchymal transition in cancer pathology. Pathology. 39:305–318. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Micalizzi DS, Farabaugh SM and Ford HL: Epithelial-mesenchymal transition in cancer: parallels between normal development and tumor progression. J Mammary Gland Biol Neoplasia. 15:117–134. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Lee JM, Dedhar S, Kalluri R and Thompson EW: The epithelial-mesenchymal transition: new insights in signaling, development, and disease. J Cell Biol. 172:973–981. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Li T, Li YG and Pu DM: Matrix metalloproteinase-2 and -9 expression correlated with angiogenesis in human adenomyosis. Gynecol Obstet Invest. 62:229–235. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Hung WC and Chang HC: Indole-3-carbinol inhibits Sp1-induced matrix metalloproteinase-2 expression to attenuate migration and invasion of breast cancer cells. J Agric Food Chem. 57:76–82. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Radisky ES and Radisky DC: Matrix metalloproteinase-induced epithelial-mesecnchymal transition in breast cancer. J Mammary Gland Biol Neoplasia. 15:201–212. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Lin CY, Tsai PH, Kandaswami CC, Lee PP, Huang CJ, Hwang JJ and Lee MT: Matrix metalloproteinase-9 cooperates with transcription factor Snail to induce epithelial-mesenchymal transition. Cancer Sci. 102:815–827. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Cary LA, Chang JF and Guan JL: Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J Cell Sci. 109:1787–1794. 1996.PubMed/NCBI

31 

Zhao X and Guan JL: Focal adhesion kinase and its signaling pathways in cell migration and angiogenesis. Adv Drug Deliv Rev. 63:610–615. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Ilic D, Furuta Y, Kanazawa S, Takeda N, Sobue K, Nakatsuji N, Nomura S, Fujimoto J, Okada M and Yamamoto T: Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature. 377:539–544. 1995. View Article : Google Scholar : PubMed/NCBI

33 

Cicchini C, Laudadio I, Citarella F, Carazzari M, Steindler C, Conigliaro A, Fantoni A, Amicone L and Tripodi M: TGFbeta-induced EMT requires focal adhesion kinase (FAK) signaling. Exp Cell Res. 314:143–152. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Deng B, Yang X, Liu J, He F, Zhu Z and Zhang C: Focal adhesion kinase mediates TGF-beta1-induced renal tubular epithelial-to-mesenchymal transition in vitro. Mol Cell Biochem. 340:21–29. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Bonnesen C, Eggleston IM and Hayes JD: Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines. Cancer Res. 61:6120–6130. 2001.PubMed/NCBI

36 

Bradlow HL: Indole-3-carbinol as a chemoprotective agent in breast and prostate cancer. In Vivo. 22:441–445. 2008.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ho J, Jun W, Choue R and Lee J: I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells. Mol Med Rep 7: 384-388, 2013.
APA
Ho, J., Jun, W., Choue, R., & Lee, J. (2013). I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells. Molecular Medicine Reports, 7, 384-388. https://doi.org/10.3892/mmr.2012.1198
MLA
Ho, J., Jun, W., Choue, R., Lee, J."I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells". Molecular Medicine Reports 7.2 (2013): 384-388.
Chicago
Ho, J., Jun, W., Choue, R., Lee, J."I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells". Molecular Medicine Reports 7, no. 2 (2013): 384-388. https://doi.org/10.3892/mmr.2012.1198
Copy and paste a formatted citation
x
Spandidos Publications style
Ho J, Jun W, Choue R and Lee J: I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells. Mol Med Rep 7: 384-388, 2013.
APA
Ho, J., Jun, W., Choue, R., & Lee, J. (2013). I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells. Molecular Medicine Reports, 7, 384-388. https://doi.org/10.3892/mmr.2012.1198
MLA
Ho, J., Jun, W., Choue, R., Lee, J."I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells". Molecular Medicine Reports 7.2 (2013): 384-388.
Chicago
Ho, J., Jun, W., Choue, R., Lee, J."I3C and ICZ inhibit migration by suppressing the EMT process and FAK expression in breast cancer cells". Molecular Medicine Reports 7, no. 2 (2013): 384-388. https://doi.org/10.3892/mmr.2012.1198
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