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Article

β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4

  • Authors:
    • Xinsheng Lu
    • Yi Wang
    • Hongmei Luo
    • Weibin Qiu
    • Hu Han
    • Xian Chen
    • Luoyan Yang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Urology, Second Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China, Department of Histology and Embryology, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 513-518
    |
    Published online on: November 28, 2012
       https://doi.org/10.3892/mmr.2012.1206
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Abstract

β-elemene, a non-cytotoxic antitumor reagent, inhibits the growth, proliferation and DNA synthesis of multiple types of malignant cells, resulting in the apoptosis or suppressed vascularization of tumors. β-elemene is also able to enhance the immunogenicity of the tumor cells and ameliorate systematic cellular immunity in the tumor‑bearing body. Moreover, β-elemene has the advantages of high efficiency, safety and low possibility of drug tolerance over other antitumor agents used in antitumor treatment. Therefore, β-elemene has great potential in clinical applications. However, the mechanism of β-elemene antitumor activity is largely unknown. The aim of this study was to investigate whether β-elemene is able to repress the proliferation of T24 bladder carcinoma cells through regulation of the expression of the tumor suppressor gene, Smad4. Results of a methylthiazolyl tetrazolium (MTT) assay indicated that the proliferation of T24 cells was repressed by β-elemene in a time- and concentration‑dependent manner. The lowest concentration of β-elemene to inhibit cell survival by >50% was determined using IC50 software. Microscopic observation also demonstrated the potential of β-elemene to induce the apoptosis of cancer cells. Western blot and RT-PCR analyses revealed that the expression of the Smad4 protein and mRNA was upregulated by treatment with β-elemene. Our results revealed that β-elemene was able to upregulate the expression of Smad4 in tumor cells to inhibit the proliferation of these cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lu X, Wang Y, Luo H, Qiu W, Han H, Chen X and Yang L: β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4. Mol Med Rep 7: 513-518, 2013.
APA
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., & Yang, L. (2013). β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4. Molecular Medicine Reports, 7, 513-518. https://doi.org/10.3892/mmr.2012.1206
MLA
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., Yang, L."β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4". Molecular Medicine Reports 7.2 (2013): 513-518.
Chicago
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., Yang, L."β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4". Molecular Medicine Reports 7, no. 2 (2013): 513-518. https://doi.org/10.3892/mmr.2012.1206
Copy and paste a formatted citation
x
Spandidos Publications style
Lu X, Wang Y, Luo H, Qiu W, Han H, Chen X and Yang L: β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4. Mol Med Rep 7: 513-518, 2013.
APA
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., & Yang, L. (2013). β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4. Molecular Medicine Reports, 7, 513-518. https://doi.org/10.3892/mmr.2012.1206
MLA
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., Yang, L."β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4". Molecular Medicine Reports 7.2 (2013): 513-518.
Chicago
Lu, X., Wang, Y., Luo, H., Qiu, W., Han, H., Chen, X., Yang, L."β-elemene inhibits the proliferation of T24 bladder carcinoma cells through upregulation of the expression of Smad4". Molecular Medicine Reports 7, no. 2 (2013): 513-518. https://doi.org/10.3892/mmr.2012.1206
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