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Article

Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells

  • Authors:
    • Bao-Liang Li
    • Xiao-Shan Zhao
    • Xiao-Min Sun
    • Jun Li
    • Jing Chen
    • Ren Luo
    • Qi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Digestive Diseases, Changzhou Hospital of TCM, Changzhou 213003, P.R. China, Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R. China
  • Pages: 454-460
    |
    Published online on: December 3, 2012
       https://doi.org/10.3892/mmr.2012.1219
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Abstract

To investigate the effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells (HSkMCs), HSkMCs were cultured in vitro and treated with serum from healthy and sub-healthy subjects independently. Total RNA was extracted, purified and used for reverse transcription into cDNA. This was labeled with one of two fluorescence dyes, Cy3 or Cy5, and hybridized with a Human Genome Array carrying 8064 target genes. The differentially expressed genes were selected with the aid of software. There were 86 differentially expressed genes identified between the sub-healthy subjects and the healthy individuals, of which 60 were upregulated and 26 were downregulated compared with those of the healthy individuals. These genes were mainly associated with the cell cycle, membrane channels, protein transport, energy metabolism and apoptosis. Our results suggest that serum from sub-healthy subjects with fatigue may change the gene expression profile of HSkMCs.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Zhao X, Sun X, Li J, Chen J, Luo R and Zhang Q: Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells. Mol Med Rep 7: 454-460, 2013.
APA
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., & Zhang, Q. (2013). Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells. Molecular Medicine Reports, 7, 454-460. https://doi.org/10.3892/mmr.2012.1219
MLA
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., Zhang, Q."Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells". Molecular Medicine Reports 7.2 (2013): 454-460.
Chicago
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., Zhang, Q."Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells". Molecular Medicine Reports 7, no. 2 (2013): 454-460. https://doi.org/10.3892/mmr.2012.1219
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Zhao X, Sun X, Li J, Chen J, Luo R and Zhang Q: Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells. Mol Med Rep 7: 454-460, 2013.
APA
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., & Zhang, Q. (2013). Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells. Molecular Medicine Reports, 7, 454-460. https://doi.org/10.3892/mmr.2012.1219
MLA
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., Zhang, Q."Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells". Molecular Medicine Reports 7.2 (2013): 454-460.
Chicago
Li, B., Zhao, X., Sun, X., Li, J., Chen, J., Luo, R., Zhang, Q."Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells". Molecular Medicine Reports 7, no. 2 (2013): 454-460. https://doi.org/10.3892/mmr.2012.1219
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