Function of ssDNA aptamer and aptamer pool against Mycobacterium tuberculosis in a mouse model

Chen,Fan; Zhou,Jing; Huang,Yong-Hao; Huang,Feng-Ying; Liu,Qiang; Fang,Zhigang; Yang,Sheng; Xiong,Min; Lin,Ying-Ying; Tan,Guang-Hong

doi:10.3892/mmr.2012.1229

February 2013 Volume 7 Issue 2

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February 2013 Volume 7 Issue 2

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Article

Function of ssDNA aptamer and aptamer pool against Mycobacterium tuberculosis in a mouse model

Authors:
- Fan Chen
- Jing Zhou
- Yong-Hao Huang
- Feng-Ying Huang
- Qiang Liu
- Zhigang Fang
- Sheng Yang
- Min Xiong
- Ying-Ying Lin
- Guang-Hong Tan
View Affiliations / Copyright

Affiliations: Faculty of Life Sciences, Hubei University, Wuchang, Wuhan 430062, P.R. China, Wuhan Tuberculosis Dispensary, Qiaokou, Wuhan 430030, P.R. China, Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, P.R. China, Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, P.R. China
Pages: 669-673
|
Published online on: December 12, 2012

https://doi.org/10.3892/mmr.2012.1229
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Abstract

Novel antibacterial agents against Mycobacterium tuberculosis (MTB) are crucial due to the high infection and mortality rates associated with the disease. Our previous study confirmed that aptamers from a whole bacterium obtained by the Systematic Evolution of Ligands by Exponential Enrichment method specifically bound to the MTB virulent strain (H37Rv). In the present study, the function of aptamers against MTB in a mouse model was further determined. It was demonstrated that the NK2 aptamer has marked inhibitory effects on the adhesion/invasion of H37Rv to macrophages in vitro and stimulates intracellular IFN-γ production in CD4+ T-cells. The aptamer pool exhibited the strongest inhibitory effect on H37Rv adhesion/invasion to CD8+ T-cells in vitro compared with all aptamers-treated and control groups. Histopathological examination of lung biopsy specimens revealed a correlation between aptamer presence and lower pulmonary alveoli fusion, swelling and more prominent air spaces. Acid-fast staining of biopsy specimens from the lungs of the mice demonstrated parallel treatment effects. Results of the present study indicate that the 10th pool aptamers and NK2 may play an active role against H37Rv, however, the effect was different in vivo and in vitro. The treatment effect of 10th pool aptamers was found to be better in comparison to NK2 in vivo. Additional target sites involved in pathogenicity of H37Rv were also revealed and the NK2 binding site and aptamers, including the 10th pool aptamers, may antagonize these sites. Further studies are required to screen for other valuable aptamers which may be used as therapeutic drugs in combination with NK2.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Function of ssDNA aptamer and aptamer pool against Mycobacterium tuberculosis in a mouse model

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