Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro

Zhang,Chang-Chun; Zhou,Jian-Sheng; Hu,Jian-Guo; Wang,Xiang; Zhou,Xin-She; Sun,Bao-An; Shao,Chen; Lin,Qinghua

doi:10.3892/mmr.2012.1238

February 2013 Volume 7 Issue 2

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February 2013 Volume 7 Issue 2

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Article

Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro

Authors:
- Chang-Chun Zhang
- Jian-Sheng Zhou
- Jian-Guo Hu
- Xiang Wang
- Xin-She Zhou
- Bao-An Sun
- Chen Shao
- Qinghua Lin
View Affiliations / Copyright

Affiliations: Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, P.R. China, Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu 233004, P.R. China, Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, P.R. China
Pages: 441-444
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Published online on: December 17, 2012

https://doi.org/10.3892/mmr.2012.1238
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Abstract

Excessive apoptosis in intervertebral disc (IVD) cells is important in IVD degeneration. Interleukin (IL)-1β has been shown to induce apoptosis in these cells. However, whether insulin-like growth factor-1 (IGF-1) inhibits IL-1β-induced apoptosis in the nucleus pulposus remains unclear. The purpose of this study was to investigate the effects of IGF-1 on IL-1β-induced apoptosis in the nucleus pulposus. Cells isolated from the nucleus pulposus were grown in culture to a monolayer. These cells were identified using immunohistochemistry for type II collagen and toluidine blue staining for glycosaminoglycans. Following exposure to IGF-1 or IL-1β, the cells were observed using light microscopy. Giemsa staining, TdT-mediated dUTP-biotin nick end-labeling (TUNEL) and flow cytometry (FCM) were used to detect the rate of early cell death, which served as an indicator of apoptosis. In the IL-1β group, a large number of these cells underwent apoptosis and demonstrated morphological changes associated with apoptosis. A small proportion of cells exposed to IGF-1 alone underwent apoptosis. No obvious signs of apoptosis were observed in the control group. TUNEL results revealed that the rate of apoptosis in the IGF-1 group was significantly reduced compared with that in the IL-1β group (P<0.01), confirmed using FCM. Compared with the control group, the apoptotic rate was also significantly increased in IL-1β-exposed cells (P<0.01). These findings strongly suggested that IGF-1 inhibits IL-1β-induced apoptosis in the nucleus pulposus.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro

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