Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro

  • Authors:
    • Chang-Chun Zhang
    • Jian-Sheng Zhou
    • Jian-Guo Hu
    • Xiang Wang
    • Xin-She Zhou
    • Bao-An Sun
    • Chen Shao
    • Qinghua Lin
  • View Affiliations

  • Published online on: December 17, 2012     https://doi.org/10.3892/mmr.2012.1238
  • Pages: 441-444
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Abstract

Excessive apoptosis in intervertebral disc (IVD) cells is important in IVD degeneration. Interleukin (IL)-1β has been shown to induce apoptosis in these cells. However, whether insulin-like growth factor-1 (IGF-1) inhibits IL-1β-induced apoptosis in the nucleus pulposus remains unclear. The purpose of this study was to investigate the effects of IGF-1 on IL-1β-induced apoptosis in the nucleus pulposus. Cells isolated from the nucleus pulposus were grown in culture to a monolayer. These cells were identified using immuno­histochemistry for type II collagen and toluidine blue staining for glycosaminoglycans. Following exposure to IGF-1 or IL-1β, the cells were observed using light microscopy. Giemsa staining, TdT-mediated dUTP-biotin nick end-labeling (TUNEL) and flow cytometry (FCM) were used to detect the rate of early cell death, which served as an indicator of apoptosis. In the IL-1β group, a large number of these cells underwent apoptosis and demonstrated morphological changes associated with apoptosis. A small proportion of cells exposed to IGF-1 alone underwent apoptosis. No obvious signs of apoptosis were observed in the control group. TUNEL results revealed that the rate of apoptosis in the IGF-1 group was significantly reduced compared with that in the IL-1β group (P<0.01), confirmed using FCM. Compared with the control group, the apoptotic rate was also significantly increased in IL-1β-exposed cells (P<0.01). These findings strongly suggested that IGF-1 inhibits IL-1β-induced apoptosis in the nucleus pulposus.
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February 2013
Volume 7 Issue 2

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Spandidos Publications style
Zhang C, Zhou J, Hu J, Wang X, Zhou X, Sun B, Shao C and Lin Q: Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro. Mol Med Rep 7: 441-444, 2013
APA
Zhang, C., Zhou, J., Hu, J., Wang, X., Zhou, X., Sun, B. ... Lin, Q. (2013). Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro. Molecular Medicine Reports, 7, 441-444. https://doi.org/10.3892/mmr.2012.1238
MLA
Zhang, C., Zhou, J., Hu, J., Wang, X., Zhou, X., Sun, B., Shao, C., Lin, Q."Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro". Molecular Medicine Reports 7.2 (2013): 441-444.
Chicago
Zhang, C., Zhou, J., Hu, J., Wang, X., Zhou, X., Sun, B., Shao, C., Lin, Q."Effects of IGF-1 on IL-1β-induced apoptosis in rabbit nucleus pulposus cells in vitro". Molecular Medicine Reports 7, no. 2 (2013): 441-444. https://doi.org/10.3892/mmr.2012.1238