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Article

Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome

  • Authors:
    • Jin-Jian Lu
    • Zhen Yang
    • De-Zhao Lu
    • Xing-De Wo
    • Jia‑Jie  Shi
    • Tao‑Qi Lin
    • Mi-Mi Wang
    • Yi Li
    • Li-Hua Tang
  • View Affiliations / Copyright

    Affiliations: College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China
  • Pages: 429-433
    |
    Published online on: May 8, 2012
       https://doi.org/10.3892/mmr.2012.906
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Abstract

Artemisinin, the active ingredient of the Chinese medicinal herb Artemisia annua L., and its derivatives (ARTs) are currently widely used as anti-malarial drugs around the world. In this study, we found that dihydroartemisinin (DHA), one of the main active metabolites of ARTs, inhibited the proliferation of human hepatocarcinoma BEL-7402 cells in a concentration-dependent manner. To interpret the mechanisms involved, an analysis of the mitochondrial proteome was performed employing two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Seven mitochondrial proteins including fumarate hydratase, 60 kDa heat shock protein, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, two subunits of ATP synthase and NADPH:adrenodoxin oxidoreductase were identified to be differentially expressed between the control and DHA-treated groups. Our results indicate that the imbalance of energy metabolism induced by DHA may contribute, at least in part, to its anti-cancer potential in BEL-7402 cells.
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1 

White NJ: Qinghaosu (artemisinin): the price of success. Science. 320:330–334. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Lu JJ, Meng LH, Cai YJ, et al: Dihydroartemisinin induces apoptosis in HL-60 leukemia cells dependent of iron and p38 mitogen-activated protein kinase activation but independent of reactive oxygen species. Cancer Biol Ther. 7:1017–1023. 2008. View Article : Google Scholar

3 

Efferth T, Sauerbrey A, Olbrich A, et al: Molecular modes of action of artesunate in tumor cell lines. Mol Pharmacol. 64:382–394. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Efferth T, Olbrich A and Bauer R: mRNA expression profiles for the response of human tumor cell lines to the antimalarial drugs artesunate, arteether, and artemether. Biochem Pharmacol. 64:617–623. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Li LN, Zhang HD, Yuan SJ, Tian ZY, Wang L and Sun ZX: Artesunate attenuates the growth of human colorectal carcinoma and inhibits hyperactive Wnt/beta-catenin pathway. Int J Cancer. 121:1360–1365. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Jiao Y, Ge CM, Meng QH, Cao JP, Tong J and Fan SJ: Dihydroartemisinin is an inhibitor of ovarian cancer cell growth. Acta Pharmacol Sin. 28:1045–1056. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Chen T, Li M, Zhang R and Wang H: Dihydroartemisinin induces apoptosis and sensitizes human ovarian cancer cells to carboplatin therapy. J Cell Mol Med. 13:1358–1370. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Tan W, Lu J, Huang M, et al: Anti-cancer natural products isolated from chinese medicinal herbs. Chin Med. 6:272011. View Article : Google Scholar : PubMed/NCBI

9 

Mercer AE, Maggs JL, Sun XM, et al: Evidence for the involvement of carbon-centered radicals in the induction of apoptotic cell death by artemisinin compounds. J Biol Chem. 282:9372–9382. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Efferth T, Benakis A, Romero MR, et al: Enhancement of cytotoxicity of artemisinins toward cancer cells by ferrous iron. Free Radic Biol Med. 37:998–1009. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Efferth T: Molecular pharmacology and pharmacogenomics of artemisinin and its derivatives in cancer cells. Curr Drug Targets. 7:407–421. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Singh NP and Lai H: Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci. 70:49–56. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Lu JJ, Chen SM, Zhang XW, Ding J and Meng LH: The anti-cancer activity of dihydroartemisinin is associated with induction of iron-dependent endoplasmic reticulum stress in colorectal carcinoma HCT116 cells. Invest New Drugs. 29:1276–1283. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Lu YY, Chen TS, Qu JL, Pan WL, Sun L and Wei XB: Dihydroartemisinin (DHA) induces caspase-3-dependent apoptosis in human lung adenocarcinoma ASTC-a-1 cells. J Biomed Sci. 16:162009. View Article : Google Scholar : PubMed/NCBI

15 

Chen H, Sun B, Pan S, Jiang H and Sun X: Dihydroartemisinin inhibits growth of pancreatic cancer cells in vitro and in vivo. Anticancer Drugs. 20:131–140. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Lu JJ, Meng LH, Shankavaram UT, et al: Dihydroartemisinin accelerates c-MYC oncoprotein degradation and induces apoptosis in c-MYC-overexpressing tumor cells. Biochem Pharmacol. 80:22–30. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Du JH, Zhang HD, Ma ZJ and Ji KM: Artesunate induces oncosis-like cell death in vitro and has antitumor activity against pancreatic cancer xenografts in vivo. Cancer Chemother Pharmacol. 65:895–902. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Li W, Mo W, Shen D, et al: Yeast model uncovers dual roles of mitochondria in action of artemisinin. PLoS Genet. 1:e362005. View Article : Google Scholar : PubMed/NCBI

19 

Reungpatthanaphong P and Mankhetkorn S: Modulation of multidrug resistance by artemisinin, artesunate and dihydroartemisinin in K562/adr and GLC4/adr resistant cell lines. Biol Pharm Bull. 25:1555–1561. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Lu JJ, Chen SM, Ding J and Meng LH: Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line. Mol Cell Biochem. 360:329–337. 2012. View Article : Google Scholar : PubMed/NCBI

21 

King A, Selak MA and Gottlieb E: Succinate dehydrogenase and fumarate hydratase: linking mitochondrial dysfunction and cancer. Oncogene. 25:4675–4682. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Li Z, Zhao X, Yang J and Wei Y: Proteomics profile changes in cisplatin-treated human ovarian cancer cell strain. Sci China C Life Sci. 48:648–657. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Castagna A, Antonioli P, Astner H, et al: A proteomic approach to cisplatin resistance in the cervix squamous cell carcinoma cell line A431. Proteomics. 4:3246–3267. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Lai MY, Hour MJ, Wing-Cheung Leung H, Yang WH and Lee HZ: Chaperones are the target in aloe-emodin-induced human lung nonsmall carcinoma H460 cell apoptosis. Eur J Pharmacol. 573:1–10. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Chen ST, Pan TL, Tsai YC and Huang CM: Proteomics reveals protein profile changes in doxorubicin--treated MCF-7 human breast cancer cells. Cancer Lett. 181:95–107. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Cappello F, Conway de Macario E, Marasa L, Zummo G and Macario AJ: Hsp60 expression, new locations, functions and perspectives for cancer diagnosis and therapy. Cancer Biol Ther. 7:801–809. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lu J, Yang Z, Lu D, Wo X, Shi JJ, Lin TQ, Wang M, Li Y and Tang L: Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Mol Med Rep 6: 429-433, 2012.
APA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T. ... Tang, L. (2012). Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Molecular Medicine Reports, 6, 429-433. https://doi.org/10.3892/mmr.2012.906
MLA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6.2 (2012): 429-433.
Chicago
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6, no. 2 (2012): 429-433. https://doi.org/10.3892/mmr.2012.906
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Yang Z, Lu D, Wo X, Shi JJ, Lin TQ, Wang M, Li Y and Tang L: Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Mol Med Rep 6: 429-433, 2012.
APA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T. ... Tang, L. (2012). Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome. Molecular Medicine Reports, 6, 429-433. https://doi.org/10.3892/mmr.2012.906
MLA
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6.2 (2012): 429-433.
Chicago
Lu, J., Yang, Z., Lu, D., Wo, X., Shi, J., Lin, T., Wang, M., Li, Y., Tang, L."Dihydroartemisinin-induced inhibition of proliferation in BEL-7402 cells: An analysis of the mitochondrial proteome". Molecular Medicine Reports 6, no. 2 (2012): 429-433. https://doi.org/10.3892/mmr.2012.906
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