Contactin 1 (CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma

Wu,He-Ming; Cao,Wei; Ye,Dongxia; Ren,Guo-Xin; Wu,Yu-Nong; Guo,Wei

doi:10.3892/mmr.2012.910

August 2012 Volume 6 Issue 2

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August 2012 Volume 6 Issue 2

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Article Open Access

Contactin 1 (CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma

Authors:
- He-Ming Wu
- Wei Cao
- Dongxia Ye
- Guo-Xin Ren
- Yu-Nong Wu
- Wei Guo
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Affiliations: Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial - Head and Neck Oncology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China, Institute of Stomatology, Department of Oral and Maxillofacial Surgery, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China

Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 265-270
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Published online on: May 9, 2012

https://doi.org/10.3892/mmr.2012.910
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Abstract

The contactin 1 (CNTN1) gene exerts oncogene‑like activities and its expression has been linked to several human malignancies. In this study, a possible association between CNTN1 expression and clinicopathological parameters and clinical outcomes in patients with oral squamous cell carcinoma (OSCC) was examined. CNTN1 protein expression was evaluated by immunohistochemistry in OSCC tissues of 45 patients. For the immunohistochemical assessment of CNTN1 expression, the cytoplasmic staining labeling index was analyzed using a semiquantitative score. The association between CNTN1 protein levels and clinicopathological factors was analyzed using the Mann-Whitney U test for categorical variables and the Kruskal-Wallis test for continuous variables. The effects of CNTN1 expression on overall and disease-free survival were assessed by using univariate survival analysis. The transcript levels of CNTN1 were detected in OSCC cell lines. In addition, specific siRNA against CNTN1 was applied to investigate the effect exerted by CNTN1 ablation on OSCC cell lines by proliferation and invasion assays in vitro. During follow-up, 16 patients (35.56%) had succumbed to OSCC; the median follow-up of patients was 5.0 years (range, 0.2-8.3). A high expression of CNTN1 was markedly associated with the regional lymph node metastasis of patients with OSCC (P=0.006). CNTN1 expression was significantly associated with overall survival of patients with OSCC (P=0.032; log-rank test) and disease-free survival of patients with OSCC (P=0.038; log-rank test). In addition, CNTN1 ablation notably suppressed the invasion potential of OSCC cell lines, but there was no significant change in the proliferation of OSCC cell lines by CNTN1 knockdown in vitro. The study supports CNTN1 as a novel predictor of regional lymph node metastasis in patients with OSCC and a prognostic marker for OSCC in patients.

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1	Jemal A, Siegel R, Ward E, et al: Cancer Statistics. CA Cancer J Clin. 59:225–249. 2009.
2	Warnakulasuriya S: Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 45:309–316. 2009.
3	Falk J, Bonnon C, Girault JA and Faivre-Sarrailh C: F3/contactin, a neuronal cell adhesion molecule implicated in axogenesis and myelination. Biol Cell. 94:327–334. 2002.
4	Hu QD, Ang BT, Karsak M, et al: F3/contactin acts as a functional ligand for Notch during oligodendrocyte maturation. Cell. 115:163–175. 2003.
5	Prag S, Lepekin EA, Kolkova K, et al: NCAM regulates cell motility. J Cell Sci. 115:283–292. 2002.
6	Shtutman M, Levina E, Ohouo P, Baig M and Roninson IB: Cell adhesion molecule L1 disrupts E-cadherin-containing adherens junctions and increases scattering and motility of MCF7 breast carcinoma cells. Cancer Res. 66:11370–11380. 2006.
7	Su JL, Yang CY, Shih JY, et al: Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer Res. 66:2553–2561. 2006.
8	Su JL, Yang PC, Shih JY, et al: The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells. Cancer Cell. 9:209–223. 2006.
9	Sobin LH and Fleming ID: TNM Classification of Malignant Tumors, fifth edition (1997). Union Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer. 80:1803–1804. 1997.
10	Pindborg JJ, Reichart PA, Smith CJ, et al: Histological Typing of Cancer and Precancer of the Oral Mucosa. 2nd edition. Springer-Verlag; Berlin: pp. 24–40. 1997
11	Mignogna MD, Fedele S and Lo Russo L: The World Cancer Report and the burden of oral cancer. Eur J Cancer Prev. 13:139–142. 2004.
12	Reid RA, Bronson DD, Young KM and Hemperly JJ: Identification and characterization of the human cell adhesion molecule contactin. Brain Res Mol Brain Res. 21:1–8. 1994.
13	Mikami T, Yasunaga D and Kitagawa H: Contactin-1 is a functional receptor for neuroregulatory chondroitin sulfate-E. J Biol Chem. 284:4494–4499. 2009.
14	Eckerich C, Zapf S and Ulbricht U: Contactin is expressed in human astrocytic gliomas and mediates repulsive effects. Glia. 53:1–12. 2006.
15	Compton AG, Albrecht DE and Seto JT: Mutations in contactin-1, a neural adhesion and neuromuscular junction protein, cause a familial form of lethal congenital myopathy. Am J Hum Genet. 83:714–724. 2008.
16	Lehembre F, Yilmaz M and Wicki A: NCAM-induced focal adhesion assembly: a functional switch upon loss of E-cadherin. EMBO J. 27:2603–2615. 2008.
17	Van Kilsdonk JW, Wilting RH and Bergers M: Attenuation of melanoma invasion by a secreted variant of activated leukocyte cell adhesion molecule. Cancer Res. 68:3671–3679. 2008.
18	Gavert N, Sheffer M and Raveh S: Expression of L1-CAM and ADAM10 in human colon cancer cells induces metastasis. Cancer Res. 67:7703–7712. 2007.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Contactin 1 (CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma

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