Open Access

Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation and invasiveness of U87 glioma cells

  • Authors:
    • Su Y. Feng
    • Chang G. Dong
    • William K.K. Wu
    • Xiao J. Wang
    • Jian Qiao
    • Jun F. Shao
  • View Affiliations

  • Published online on: May 15, 2012     https://doi.org/10.3892/mmr.2012.915
  • Pages: 275-281
  • Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Glioma is a highly fatal malignant disease and its treatment options are limited. microRNAs represent a novel target for the treatment of cancer. In the present study, we used a lentiviral vector to stably express anti-microRNAs targeting the oncogenic miR-27a in U87 glioma cells. The stable expression of anti-miR-27a significantly reduced the proliferation and increased the accumulation of U87 cells in the sub-G1 phase as determined by Cell Counting kit-8 (CCK-8) assays and flow cytometry, respectively. Results from the Matrigel transwell assay also indicated that the inhibition of miR-27a substantially impaired the invasiveness of U87 cells. By combining bioinformatic and proteomic approaches, we identified the mRNAs of 8 proteins upregulated in anti-miR-27a-expressing U87 cells as putative direct targets of miR-27a. Collectively, these data suggest that the lentiviral expression of anti-miR-27a is a feasible approach for the suppression of malignant phenotypes of glioma cells.
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August 2012
Volume 6 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Feng SY, Dong CG, Wu WK, Wang XJ, Qiao J and Shao JF: Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation and invasiveness of U87 glioma cells. Mol Med Rep 6: 275-281, 2012
APA
Feng, S.Y., Dong, C.G., Wu, W.K., Wang, X.J., Qiao, J., & Shao, J.F. (2012). Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation and invasiveness of U87 glioma cells. Molecular Medicine Reports, 6, 275-281. https://doi.org/10.3892/mmr.2012.915
MLA
Feng, S. Y., Dong, C. G., Wu, W. K., Wang, X. J., Qiao, J., Shao, J. F."Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation and invasiveness of U87 glioma cells". Molecular Medicine Reports 6.2 (2012): 275-281.
Chicago
Feng, S. Y., Dong, C. G., Wu, W. K., Wang, X. J., Qiao, J., Shao, J. F."Lentiviral expression of anti-microRNAs targeting miR-27a inhibits proliferation and invasiveness of U87 glioma cells". Molecular Medicine Reports 6, no. 2 (2012): 275-281. https://doi.org/10.3892/mmr.2012.915