Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August 2012 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August 2012 Volume 6 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts

  • Authors:
    • Akira Kondo
    • Haruhiko Tokuda
    • Jun Mizutani
    • Rie Matsushima-Nishiwaki
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan, Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
  • Pages: 421-425
    |
    Published online on: May 15, 2012
       https://doi.org/10.3892/mmr.2012.916
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

It is known that Wnt3a affects bone metabolism via the canonical Wnt/β-catenin signaling pathway. We have previously shown that prostaglandin F2α (PGF2α) stimulates the synthesis of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) via mitogen-activated protein (MAP) kinases, including p44/p42 MAP kinase, p38 MAP kinase and stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the effects of Wnt3a on the synthesis of VEGF or IL-6 stimulated by PGF2α in MC3T3-E1 cells using an ELISA kit and various antibodies. Cells were cultured and pretreated with various doses of Wnt3a or SB216763, an inhibitor of glycogen synthase kinase 3β, prior to western blotting. Wnt3a significantly enhanced the PGF2α-stimulated VEGF release but had little effect on the PGF2α-stimulated IL-6 release. SB216763 markedly amplified the PGF2α-stimulated VEGF release without affecting the IL-6 release, similar to Wnt3a. Wnt3a failed to affect the PGF2α-induced phosphorylation of p44/p42 MAP kinase, p38 MAP kinase or SAPK/JNK. These results strongly suggest that Wnt3a upregulates VEGF synthesis stimulated by PGF2α via activation of the canonical pathway in osteoblasts without affecting IL-6 synthesis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Pilbeam CC, Harrison JR and Raisz LG: Prostaglandins and bone metabolism. Principles of Bone Biology. Bilezikian JP, Raisz LG and Rodan GA: Academic press; San Diego: pp. 715–728. 1996

2 

Hikiji H, Takato T, Shimizu T and Ishii S: The roles of prostanoids, leukotrienes, and platelet-activating factor in bone metabolism and disease. Prog Lipid Res. 47:107–126. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Miwa M, Tokuda H, Tsushita K, Kotoyori J, Takahashi Y, Ozaki N, Kozawa O and Oiso Y: Involvement of pertussis-toxin-sensitive GTP-binding protein in prostaglandin F2α-induced phosphoinositide hydrolysis in osteoblast-like cells. Biochem Biophys Res Commun. 171:1229–1235. 1990.

5 

Kozawa O, Suzuki A, Kotoyori J, Tokuda H, Watanabe Y, Ito Y and Oiso Y: Prostaglandin F2α-activates phospholipase D independently from activation of protein kinase C in osteoblast-like cells. J Cell Biochem. 55:373–379. 1994.

6 

Tokuda H, Harada A, Hirade K, Matsuno H, Ito H, Kato K, Oiso Y and Kozawa O: Incadronate amplifies prostaglandin F2α-induced vascular endothelial growth factor in osteoblasts: enhancement of MAP kinase activity. J Biol Chem. 278:18930–18937. 2003.

7 

Ferrara N: Vascular endothelial growth factor: basic science and clinical progress. Endoc Rev. 25:581–611. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Erlebacher A, Filvaroff EH, Gitelman SE and Derynck R: Toward a molecular understanding of skeletal development. Cell. 80:371–378. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Grellier M, Ferreira-Tojais N, Bourget C, Bareille R, Guillemot F and Amédée J: Role of vascular endothelial growth factor in the communication between human osteoprogenitors and endothelial cells. J Cell Biochem. 106:390–398. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Tokuda H, Takai S, Matsushima-Nishiwaki R, Akamatsu S, Hanai Y, Hosoi T, Harada A, Ohta T and Kozawa O: (-)-epigallocatechin gallate enhances prostaglandin F2α-induced VEGF synthesis via up-regulating SAPK/JNK activation in osteoblasts. J Cell Biochem. 100:1146–1153. 2007.PubMed/NCBI

11 

Kyriakis JM and Avruch J: Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol Rev. 81:807–869. 2001.PubMed/NCBI

12 

Kishimoto T: Interleukin-6: from basic science to medicine - 40 years in immunology. Annu Rev Immunol. 23:1–21. 2005.PubMed/NCBI

13 

Kwan Tat S, Padrines M, Théoleyre S, Heymann D and Fortun Y: IL-6, RANKL, TNF-alpha/IL-1: interrelations in bone resorption pathophysiology. Cytokine Growth Factor Rev. 15:49–60. 2004.

14 

Kozawa O, Suzuki A, Tokuda H and Uematsu T: Prostaglandin F2α stimulates interleukin-6 via activation of PKC in osteoblast-like cells. Am J Physiol. 272:E208–E211. 1997.

15 

Moon RT, Bowerman B, Boutros M and Perrimon N: The promise and perils of Wnt signalling through β-catenin. Science. 296:1644–1646. 2002.PubMed/NCBI

16 

van Amerongen R and Nusse R: Towards an integrated view of Wnt signalling in development. Development. 136:3205–3214. 2009.PubMed/NCBI

17 

Milat F and Ng KW: Is Wnt signalling the final common pathway leading to bone formation? Mol Cell Endocrinol. 310:52–62. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Rodda SJ and McMahon AP: Distinct roles for hedgehog and canonical Wnt signalling in specification, differentiation and maintenance of osteoblast progenitors. Development. 133:3231–3244. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Hu H, Hilton MJ, Tu X, Yu K, Ornitz DM and Long F: Sequential roles of Hedgehog and Wnt signalling in osteoblast development. Development. 132:49–60. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Krishnan V, Bryant HU and Macdougald OA: Regulation of bone mass by Wnt signalling. J Clin Invest. 116:1202–1209. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Almeida M, Han L, Bellido T, Manolagas SC and Kousteni S: Wnt proteins prevent apoptosis of both uncommitted osteoblast progenitors and differentiated osteoblasts by β-catenin- dependent and -independent signalling cascades involving Src/ERK and phosphatidylinositol 3-kinase/AKT. J Biol Chem. 280:41342–41351. 2005.PubMed/NCBI

22 

Sudo H, Kodama H, Amagai Y, Yamamoto S and Kasai S: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

23 

Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross-talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI

24 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and αB-crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996.

25 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

26 

Case N, Ma M, Sen B, Xie Z, Gross TS and Rubin J: β-catenin levels influence rapid mechanical responses in osteoblasts. J Biol Chem. 283:29196–29205. 2008.

27 

Carmichael J, Sugars KL, Bao YP and Rubinsztein DC: Glycogen synthase kinase-3β inhibitors prevent cellular polyglutamine toxicity caused by the Huntington’s disease mutation. J Biol Chem. 277:33791–33798. 2002.

28 

Chung YS, Baylink DJ, Srivastava AK, Amaar Y, Tapia B, Kasukawa Y and Mohan S: Effects of secreted frizzled-related protein 3 on osteoblasts in vitro. J Bone Miner Res. 19:1395–1402. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Gerber HP, Vu TH, Ryan AM, Kowalski J, Werb Z and Ferrara N: VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nature Med. 5:623–628. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Secreto FJ, Hoeppner LH and Westendorf JJ: Wnt signalling during fracture repair. Curr Osteoporos Rep. 7:64–69. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kondo A, Tokuda H, Mizutani J, Matsushima-Nishiwaki R, Kozawa O and Otsuka T: Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts. Mol Med Rep 6: 421-425, 2012.
APA
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., & Otsuka, T. (2012). Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts. Molecular Medicine Reports, 6, 421-425. https://doi.org/10.3892/mmr.2012.916
MLA
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., Otsuka, T."Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts". Molecular Medicine Reports 6.2 (2012): 421-425.
Chicago
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., Otsuka, T."Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts". Molecular Medicine Reports 6, no. 2 (2012): 421-425. https://doi.org/10.3892/mmr.2012.916
Copy and paste a formatted citation
x
Spandidos Publications style
Kondo A, Tokuda H, Mizutani J, Matsushima-Nishiwaki R, Kozawa O and Otsuka T: Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts. Mol Med Rep 6: 421-425, 2012.
APA
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., & Otsuka, T. (2012). Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts. Molecular Medicine Reports, 6, 421-425. https://doi.org/10.3892/mmr.2012.916
MLA
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., Otsuka, T."Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts". Molecular Medicine Reports 6.2 (2012): 421-425.
Chicago
Kondo, A., Tokuda, H., Mizutani, J., Matsushima-Nishiwaki, R., Kozawa, O., Otsuka, T."Wnt3a upregulates prostaglandin F2α-stimulated vascular endothelial growth factor synthesis in osteoblasts". Molecular Medicine Reports 6, no. 2 (2012): 421-425. https://doi.org/10.3892/mmr.2012.916
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team