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August 2012 Volume 6 Issue 2

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Article

Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses

  • Authors:
    • Wenhua Xu
    • Changqing Jiang
    • Xiaoying Kong
    • Ye Liang
    • Mi Rong
    • Wanshun Liu
  • View Affiliations / Copyright

    Affiliations: College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong, P.R. China, Department of Pathology, Municipal Hospital of Qingdao, Qingdao, Shandong, P.R. China
  • Pages: 385-390
    |
    Published online on: May 16, 2012
       https://doi.org/10.3892/mmr.2012.918
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Abstract

The aim of the present study was to evaluate the anticancer effects of chitooligosaccharides (COS) and N-acetyl-D-glucosamine (NAG), as well as to investigate the possible mechanisms involved. MTT assay and flow cytometry were used to evaluate the effect of various concentrations of COS and NAG on the proliferation and differentiation of peripheral blood mononuclear cells (PBMCs). In addition, sarcoma 180 cells were transplanted into mice to establish a tumor model. COS and NAG were administered by gavage of various doses. The tumor inhibition rate, thymus and spleen indexes, natural killer (NK) cell activity, and interleukin-2 (IL-2) and interferon-γ (IFN-γ) serum levels were detected. Vascular endothelial growth factor (VEGF) expression levels, an important marker of angiogenesis, were also detected. As shown by immunohistochemistry, VEGF mRNA expression was decreased following treatment with COS and NAG, indicating that COS and NAG have an inhibitory effect on the expression of VEGF. The results from this study indicate that COS administered at a dose of 100 mg/kg and NAG at a dose of 300 mg/kg or 500 mg/kg can not only promote the differentiation of PBMCs and the secretion of IL-2 and IFN-γ, but can also inhibit the expression of VEGF mRNA in sarcoma 180 tumors. Our results show that the antitumor and immunoregulatory effects of COS and NAG are dose-dependent. Furthermore, the antitumor effect is achieved by the improvement of immunoregulation indirectly.
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Copy and paste a formatted citation
Spandidos Publications style
Xu W, Jiang C, Kong X, Liang Y, Rong M and Liu W: Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses. Mol Med Rep 6: 385-390, 2012.
APA
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., & Liu, W. (2012). Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses. Molecular Medicine Reports, 6, 385-390. https://doi.org/10.3892/mmr.2012.918
MLA
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., Liu, W."Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses". Molecular Medicine Reports 6.2 (2012): 385-390.
Chicago
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., Liu, W."Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses". Molecular Medicine Reports 6, no. 2 (2012): 385-390. https://doi.org/10.3892/mmr.2012.918
Copy and paste a formatted citation
x
Spandidos Publications style
Xu W, Jiang C, Kong X, Liang Y, Rong M and Liu W: Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses. Mol Med Rep 6: 385-390, 2012.
APA
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., & Liu, W. (2012). Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses. Molecular Medicine Reports, 6, 385-390. https://doi.org/10.3892/mmr.2012.918
MLA
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., Liu, W."Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses". Molecular Medicine Reports 6.2 (2012): 385-390.
Chicago
Xu, W., Jiang, C., Kong, X., Liang, Y., Rong, M., Liu, W."Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responses". Molecular Medicine Reports 6, no. 2 (2012): 385-390. https://doi.org/10.3892/mmr.2012.918
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