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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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August 2012 Volume 6 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer

  • Authors:
    • D. Tvrdík
    • L. Staněk
    • H. Skálová
    • P. Dundr
    • Z. Velenská
    • C. Povýšil

  • View Affiliations / Copyright

    Affiliations: Institute of Pathology, 1st Faculty of Medicine, Charles University and General University Hospital, 12800 Prague 2, Czech Republic
  • Pages: 439-443
    |
    Published online on: May 17, 2012
       https://doi.org/10.3892/mmr.2012.919
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Abstract

Her2 proto-oncogene amplification and protein overexpression is observed in 20-40% of patients with breast cancer and plays a crucial role in invasive breast cancer and its treatment. In the present study, we investigated samples from 131 patients with invasive breast carcinoma. In all cases, the overexpression/amplification level of Her2 was determined using manual immunohistochemistry (IHC) and/or automatic IHC, fluorescence in situ hybridization (FISH), silver in situ hybridization (SISH) and quantitative polymerase chain reaction (qPCR). Using various methods, we demonstrated candidate methods for Her2 detection and their dependability. Our results demonstrate that these methods are highly comparable for the detection of Her2 overexpression/amplification. It was also revealed that qPCR is a valuable tool for the evaluation of Her2 gene overexpression/amplification. The results from pPCR analysis positively correlated with the results from IHC and FISH analysis. Moreover, in contrast to IHC or SISH/FISH, the results obtained by qPCR were not encumbered with any subjective error on the part of the evaluator.
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Copy and paste a formatted citation
Spandidos Publications style
Tvrdík D, Staněk L, Skálová H, Dundr P, Velenská Z and Povýšil C: Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer. Mol Med Rep 6: 439-443, 2012.
APA
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., & Povýšil, C. (2012). Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer. Molecular Medicine Reports, 6, 439-443. https://doi.org/10.3892/mmr.2012.919
MLA
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., Povýšil, C."Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer". Molecular Medicine Reports 6.2 (2012): 439-443.
Chicago
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., Povýšil, C."Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer". Molecular Medicine Reports 6, no. 2 (2012): 439-443. https://doi.org/10.3892/mmr.2012.919
Copy and paste a formatted citation
x
Spandidos Publications style
Tvrdík D, Staněk L, Skálová H, Dundr P, Velenská Z and Povýšil C: Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer. Mol Med Rep 6: 439-443, 2012.
APA
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., & Povýšil, C. (2012). Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer. Molecular Medicine Reports, 6, 439-443. https://doi.org/10.3892/mmr.2012.919
MLA
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., Povýšil, C."Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer". Molecular Medicine Reports 6.2 (2012): 439-443.
Chicago
Tvrdík, D., Staněk, L., Skálová, H., Dundr, P., Velenská, Z., Povýšil, C."Comparison of the IHC, FISH, SISH and qPCR methods for the molecular diagnosis of breast cancer". Molecular Medicine Reports 6, no. 2 (2012): 439-443. https://doi.org/10.3892/mmr.2012.919
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