Enrichment of colorectal cancer stem cells through epithelial-mesenchymal transition via CDH1 knockdown

  • Authors:
    • Jun Ye
    • Dang Wu
    • Jinwen Shen
    • Pin Wu
    • Chao Ni
    • Jing Chen
    • Jing Zhao
    • Tao Zhang
    • Xiaolei Wang
    • Jian Huang
  • View Affiliations

  • Published online on: June 7, 2012     https://doi.org/10.3892/mmr.2012.938
  • Pages: 507-512
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Abstract

Cancer stem cells (CSCs) are considered to be the origin of cancer relapse and metastasis. The better understanding of CSCs, including CSCs in human colorectal cancer (CRC), may facilitate prevention and treatment. This study aimed to establish a CSC enrichment model via the induction of epithelial-mesenchymal transition (EMT) in CRC cells. We established an EMT model using the SW480 CRC cell line by CDH1 knockdown using shRNA interference. CD24+CD44+ cells were enriched in the CDH1 knockdown cells. The cells exhibited mesenchymal morphology and expressed high levels of EMT-related proteins, which confirmed that these cells had undergone EMT. Our results further showed that the proliferation rate of the transfected cells was reduced, whereas their colony-forming capacity and tumorigenesis in vivo was significantly enhanced compared to the control cells. In conclusion, these cells were highly enriched CSCs (compared to normal CSCs) and may be used as a stable model for cancer research and anticancer drug screening.
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September 2012
Volume 6 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ye J, Wu D, Shen J, Wu P, Ni C, Chen J, Zhao J, Zhang T, Wang X, Huang J, Huang J, et al: Enrichment of colorectal cancer stem cells through epithelial-mesenchymal transition via CDH1 knockdown. Mol Med Rep 6: 507-512, 2012.
APA
Ye, J., Wu, D., Shen, J., Wu, P., Ni, C., Chen, J. ... Huang, J. (2012). Enrichment of colorectal cancer stem cells through epithelial-mesenchymal transition via CDH1 knockdown. Molecular Medicine Reports, 6, 507-512. https://doi.org/10.3892/mmr.2012.938
MLA
Ye, J., Wu, D., Shen, J., Wu, P., Ni, C., Chen, J., Zhao, J., Zhang, T., Wang, X., Huang, J."Enrichment of colorectal cancer stem cells through epithelial-mesenchymal transition via CDH1 knockdown". Molecular Medicine Reports 6.3 (2012): 507-512.
Chicago
Ye, J., Wu, D., Shen, J., Wu, P., Ni, C., Chen, J., Zhao, J., Zhang, T., Wang, X., Huang, J."Enrichment of colorectal cancer stem cells through epithelial-mesenchymal transition via CDH1 knockdown". Molecular Medicine Reports 6, no. 3 (2012): 507-512. https://doi.org/10.3892/mmr.2012.938