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Article

The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate

  • Authors:
    • Ping Huang
    • Jing Wang
    • Xi Shen
    • Qin Jiao
    • Yu Cheng
    • Bing Xie
    • Yisheng Zhong
  • View Affiliations / Copyright

    Affiliations: Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Ophthalmology, Ruijin Hospital Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 662-666
    |
    Published online on: June 13, 2012
       https://doi.org/10.3892/mmr.2012.947
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Abstract

The aim of this study was to investigate the effects of erythropoietin (EPO) on RhoA/Rho-associated kinase (ROCK) expression in rat retinal explants cultured with glutamate. After the retinal explants were cultured in serum-free R16 nutrient medium for 24 h, the retinal explants were divided into control (R16 nutrient medium), glutamate (R16 nutrient medium containing 5 mM/l glutamate) and glutamate + EPO (R16 nutrient medium containing 5 mM/l glutamate and 6.0 U/ml EPO) groups, and culturing was continued for another 72 h. The mRNA and protein expression of total RhoA, ROCK1 and ROCK2 in the retinal explants was examined by RT-PCR and western blotting, and active RhoA in the retinal explants was detected via GST-RBD binding and immunoblotting with an antibody specific to active RhoA. The total RhoA mRNA and protein expression did not differ substantially between the control, the glutamate and the glutamate + EPO groups. The glutamate increased the active RhoA, ROCK1 and ROCK2 expression in cultured retinal explants (P<0.05), whereas the expression of active RhoA, ROCK1 and ROCK2 in the glutamate + EPO group was significantly lower than that in the simple glutamate group (P<0.05) and similar to that in the control group. In conclusion, EPO downregulates active RhoA, ROCK1 and ROCK2 expression in retinal explants cultured with glutamate.
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Copy and paste a formatted citation
Spandidos Publications style
Huang P, Wang J, Shen X, Jiao Q, Cheng Y, Xie B and Zhong Y: The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Mol Med Rep 6: 662-666, 2012.
APA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., & Zhong, Y. (2012). The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Molecular Medicine Reports, 6, 662-666. https://doi.org/10.3892/mmr.2012.947
MLA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6.3 (2012): 662-666.
Chicago
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6, no. 3 (2012): 662-666. https://doi.org/10.3892/mmr.2012.947
Copy and paste a formatted citation
x
Spandidos Publications style
Huang P, Wang J, Shen X, Jiao Q, Cheng Y, Xie B and Zhong Y: The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Mol Med Rep 6: 662-666, 2012.
APA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., & Zhong, Y. (2012). The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate. Molecular Medicine Reports, 6, 662-666. https://doi.org/10.3892/mmr.2012.947
MLA
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6.3 (2012): 662-666.
Chicago
Huang, P., Wang, J., Shen, X., Jiao, Q., Cheng, Y., Xie, B., Zhong, Y."The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate". Molecular Medicine Reports 6, no. 3 (2012): 662-666. https://doi.org/10.3892/mmr.2012.947
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