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September 2012 Volume 6 Issue 3

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Article

Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model

  • Authors:
    • You-Shui Gao
    • Shang-Chun Guo
    • Hao Ding
    • Chang-Qing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 611-614
    |
    Published online on: June 20, 2012
       https://doi.org/10.3892/mmr.2012.958
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Abstract

The aim of the current study was to investigate the local expression of caspase-3 following femoral neck fractures in a canine model and to investigate its effect on the occurrence of fracture-induced osteonecrosis of the femoral head (ONFH). Eight dogs had surgically-induced femoral neck fractures on the left side which remained untreated. Radiological and histological examinations were employed to detect morphological changes of the femoral head. Immunohistochemical staining of caspase-3 was used to evaluate cell apoptosis, which may play an important role in ONFH. The results were compared to the normal side for statistical analysis. As a result, all eight dogs had ONFH, with non-union in five and malunion in three on radiological examination. Histologically, the untreated femoral heads developed osteonecrosis with an accumulation of bone marrow cell debris, empty lacunae and/or ghost nuclei in the lacunae, and an increase in the number of fat cells. Immunohistochemical staining of caspase-3 indicated that it was upregulated in fracture-induced ONFH two weeks postoperatively, which showed a statistical difference when compared to the normal side. In conclusion, the local expression of caspase-3 was upregulated in fracture-induced ONFH, suggesting that cell apoptosis is crucial in traumatic ONFH. Caspase-3 may therefore be employed as an effective and early predictor for fracture-induced ONFH.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Guo S, Ding H and Zhang C: Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model. Mol Med Rep 6: 611-614, 2012.
APA
Gao, Y., Guo, S., Ding, H., & Zhang, C. (2012). Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model. Molecular Medicine Reports, 6, 611-614. https://doi.org/10.3892/mmr.2012.958
MLA
Gao, Y., Guo, S., Ding, H., Zhang, C."Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model". Molecular Medicine Reports 6.3 (2012): 611-614.
Chicago
Gao, Y., Guo, S., Ding, H., Zhang, C."Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model". Molecular Medicine Reports 6, no. 3 (2012): 611-614. https://doi.org/10.3892/mmr.2012.958
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Guo S, Ding H and Zhang C: Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model. Mol Med Rep 6: 611-614, 2012.
APA
Gao, Y., Guo, S., Ding, H., & Zhang, C. (2012). Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model. Molecular Medicine Reports, 6, 611-614. https://doi.org/10.3892/mmr.2012.958
MLA
Gao, Y., Guo, S., Ding, H., Zhang, C."Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model". Molecular Medicine Reports 6.3 (2012): 611-614.
Chicago
Gao, Y., Guo, S., Ding, H., Zhang, C."Caspase-3 may be employed as an early predictor for fracture‑induced osteonecrosis of the femoral head in a canine model". Molecular Medicine Reports 6, no. 3 (2012): 611-614. https://doi.org/10.3892/mmr.2012.958
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