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Article

Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities

  • Authors:
    • Te Liu
    • Weiwei Cheng
    • Yongtao Gao
    • Hui Wang
    • Zhixue Liu
  • View Affiliations / Copyright

    Affiliations: School of Environmental Science and Engineering, Donghua University, Shanghai 201620, P.R. China, International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030, P.R. China , School of Life Science and Technology, Tongji University, Shanghai 200092, P.R. China
  • Pages: 535-542
    |
    Published online on: June 25, 2012
       https://doi.org/10.3892/mmr.2012.967
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Abstract

microRNAs (miRNAs) play a crucial role in tissue development and the pathology of many diseases, however, the effects and roles of miRNAs in the development of semen abnormalities in infertile males have not yet been investigated. In this study, we analyzed and compared the miRNA expression profiles of abnormal semen from 86 infertile males with normal semen from 86 healthy males using an miRNA microarray. In total, 52 miRNAs were differentially expressed between the abnormal semen of infertile males and the normal semen of healthy males. The differential expression of selected miRNAs was validated by real time qRT-PCR and northern blotting: miR-574-5p, miR-297, miR-122, miR-1275, miR-373, miR-185 and miR-193b were upregulated (fold change >1.5, p<0.001) and miR-100, miR-512-3p, miR-16, miR-19b, miR-23b and miR-26a were downregulated (fold change <0.667, p<0.001) in the semen of infertile males with semen abnormalities. In conclusion, this study provides new insights into specific miRNAs that are associated with semen abnormalities in infertile males.
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1 

Zenzmaier C, Gerth R, Gruschwitz M, Lindner H, Plas E and Berger P: Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis. Reprod Biol Endocrinol. 9:1142011. View Article : Google Scholar : PubMed/NCBI

2 

Hu W, Yang H, Sun J, et al: Polymorphisms in CYP1B1 modify the risk of idiopathic male infertility with abnormal semen quality. Clin Chim Acta. 412:1778–1782. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Chatzimeletiou K, Sioga A, Oikonomou L, et al: Semen analysis by electron and fluorescence microscopy in a case of partial hydatidiform mole reveals a high incidence of abnormal morphology, diploidy, and tetraploidy. Fertil Steril. 95:e2431–e2435. 2011. View Article : Google Scholar

4 

Moretti E, Castellini C, Mourvaki E, et al: Distribution of α- and δ-tocopherols in seminal plasma and sperm fractions of men with normal and abnormal semen parameters. J Androl. 32:232–239. 2011.

5 

Kowalczuk CI, Saunders RD and Stapleton HR: Sperm count and sperm abnormality in male mice after exposure to 2.45 GHz microwave radiation. Mutat Res. 122:155–161. 1983. View Article : Google Scholar : PubMed/NCBI

6 

Narayana K, Prashanthi N, Nayanatara A, Kumar HH, Abhilash K and Bairy KL: Effects of methyl parathion (O,O-dimethyl O-4-nitrophenyl phosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutat Res. 588:28–34. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Padmalatha Rai S and Vijayalaxmi KK: Tamoxifen citrate induced sperm shape abnormalities in the in vivo mouse. Mutat Res. 492:1–6. 2001.PubMed/NCBI

8 

Calogero AE, De Palma A, Grazioso C, et al: Aneuploidy rate in spermatozoa of selected men with abnormal semen parameters. Hum Reprod. 16:1172–1179. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Jacobsen R, Bostofte E, Engholm G, et al: Risk of testicular cancer in men with abnormal semen characteristics: cohort study. BMJ. 321:789–792. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Torra R, Sarquella J, Calabia J, et al: Prevalence of cysts in seminal tract and abnormal semen parameters in patients with autosomal dominant polycystic kidney disease. Clin J Am Soc Nephrol. 3:790–793. 2008. View Article : Google Scholar

11 

Ravnborg TL, Jensen TK, Andersson AM, Toppari J, Skakkebaek NE and Jørgensen N: Prenatal and adult exposures to smoking are associated with adverse effects on reproductive hormones, semen quality, final height and body mass index. Hum Reprod. 26:1000–1011. 2011. View Article : Google Scholar

12 

Barratt CL, Björndahl L, Menkveld R and Mortimer D: ESHRE special interest group for andrology basic semen analysis course: a continued focus on accuracy, quality, efficiency and clinical relevance. Hum Reprod. 26:3207–3212. 2011. View Article : Google Scholar

13 

Sumazin P, Yang X, Chiu HS, et al: An extensive microRNA-mediated network of RNA-RNA interactions regulates established oncogenic pathways in glioblastoma. Cell. 147:370–381. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Poulton JS, Huang YC, Smith L, et al: The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. Development. 138:1737–1745. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Lei P, Li Y, Chen X, Yang S and Zhang J: Microarray based analysis of microRNA expression in rat cerebral cortex after traumatic brain injury. Brain Res. 1284:191–201. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function (Review). Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Yoo AS, Sun AX, Li L, et al: MicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 476:228–231. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Dai Y, Diao Z, Sun H, Li R, Qiu Z and Hu Y: MicroRNA-155 is involved in the remodelling of human-trophoblast-derived HTR-8/SVneo cells induced by lipopolysaccharides. Hum Reprod. 26:1882–1891. 2011. View Article : Google Scholar : PubMed/NCBI

19 

He L and Hannon GJ: MicroRNAs: small RNAs with a big role in gene regulation (Review). Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

20 

El Ouaamari A, Baroukh N, Martens GA, Lebrun P, Pipeleers D and van Obberghen E: miR-375 targets 3′-phosphoinositide-dependent protein kinase-1 and regulates glucose-induced biological responses in pancreatic beta-cells. Diabetes. 57:2708–2717. 2008.

21 

Yang Y, Bai W, Zhang L, et al: Determination of microRNAs in mouse preimplantation embryos by microarray. Dev Dyn. 237:2315–2327. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Calin GA, Liu CG, Sevignani C, et al: MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci USA. 101:11755–11760. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Bloomston M, Frankel WL, Petrocca F, et al: MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis. JAMA. 297:1901–1908. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Yan N, Lu Y, Sun H, et al: A microarray for microRNA profiling in mouse testis tissues. Reproduction. 134:73–79. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Wang LL, Zhang Z, Li Q, et al: Ethanol exposure induces differential microRNA and target gene expression and teratogenic effects which can be suppressed by folic acid supplementation. Hum Reprod. 24:562–579. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Barshack I, Meiri E, Rosenwald S, et al: Differential diagnosis of hepatocellular carcinoma from metastatic tumors in the liver using microRNA expression. Int J Biochem Cell Biol. 42:1355–1362. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Li W, Xie L, He X, et al: Diagnostic and prognostic implications of microRNAs in human hepatocellular carcinoma. Int J Cancer. 123:1616–1622. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Cheng W, Liu T, Jiang F, et al: microRNA-155 regulates angiotensin II type 1 receptor expression in umbilical vein endothelial cells from severely pre-eclamptic pregnant women. Int J Mol Med. 27:393–399. 2011.PubMed/NCBI

29 

Zhang L, Liu T, Huang Y and Liu J: microRNA-182 inhibits the proliferation and invasion of human lung adenocarcinoma cells through its effect on human cortical actin-associated protein. Int J Mol Med. 28:381–388. 2011.PubMed/NCBI

30 

Li S, Chen X, Zhang H, et al: Differential expression of microRNAs in mouse liver under aberrant energy metabolic status. J Lipid Res. 50:1756–1765. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Wang WX, Wilfred BR, Baldwin DA, et al: Focus on RNA isolation: obtaining RNA for microRNA (miRNA) expression profiling analyses of neural tissue. Biochim Biophys Acta. 1779:749–757. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Liu HH, Tian X, Li YJ, Wu CA and Zheng CC: Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. RNA. 14:836–843. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Wu H, Neilson JR, Kumar P, et al: miRNA profiling of naive, effector and memory CD8 T cells. PLoS One. 2:e10202007. View Article : Google Scholar : PubMed/NCBI

34 

Kuhn DE, Nuovo GJ, Martin MM, et al: Human chromosome 21-derived miRNAs are overexpressed in down syndrome brains and hearts. Biochem Biophys Res Commun. 370:473–477. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Ambros V: The functions of animal microRNAs (Review). Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

36 

Du T and Zamore PD: microPrimer: the biogenesis and function of microRNA (Review). Development. 132:4645–4652. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu T, Cheng W, Gao Y, Wang H and Liu Z: Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Mol Med Rep 6: 535-542, 2012.
APA
Liu, T., Cheng, W., Gao, Y., Wang, H., & Liu, Z. (2012). Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Molecular Medicine Reports, 6, 535-542. https://doi.org/10.3892/mmr.2012.967
MLA
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6.3 (2012): 535-542.
Chicago
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6, no. 3 (2012): 535-542. https://doi.org/10.3892/mmr.2012.967
Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Cheng W, Gao Y, Wang H and Liu Z: Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Mol Med Rep 6: 535-542, 2012.
APA
Liu, T., Cheng, W., Gao, Y., Wang, H., & Liu, Z. (2012). Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities. Molecular Medicine Reports, 6, 535-542. https://doi.org/10.3892/mmr.2012.967
MLA
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6.3 (2012): 535-542.
Chicago
Liu, T., Cheng, W., Gao, Y., Wang, H., Liu, Z."Microarray analysis of microRNA expression patterns in the semen of infertile men with semen abnormalities". Molecular Medicine Reports 6, no. 3 (2012): 535-542. https://doi.org/10.3892/mmr.2012.967
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