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Molecular Medicine Reports
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September 2012 Volume 6 Issue 3

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Article

Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions

  • Authors:
    • Paweł Jóźwiak
    • Anna Krześlak
    • Lech Pomorski
    • Anna Lipińska
  • View Affiliations / Copyright

    Affiliations: Department of Cytobiochemistry, University of Łódź, 90-236 Łódź, Poland, Department of General and Oncological Surgery, Medical University of Łódź, 95-100 Zgierz, Poland
  • Pages: 601-606
    |
    Published online on: June 27, 2012
       https://doi.org/10.3892/mmr.2012.969
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Abstract

The enhancement of glucose metabolism in neoplastic cells is mediated by the overexpression of key glycolytic enzymes and glucose transporters (GLUTs). In particular, an increased expression of hypoxia-related GLUT1 and GLUT3 has been found in a variety of malignancies. The aim of this study was to examine the expression levels of GLUT1 and GLUT3 in benign and malignant thyroid tumors, as well as in non-neoplastic lesions. Analysis of the mRNA expression levels of solute carrier family 2, member 1 (SLC2A1) and solute carrier family 2, member 3 (SLC2A3) (genes coding GLUT1 and GLUT3, respectively) was performed by the real-time PCR method with fluorescent probes. GLUT1 and GLUT3 protein expression levels were determined in thyroid specimens by immunodetection after separation of proteins on 10% polyacrylamide slab gels and electrotransfer onto Immobilon-P membranes. The majority of papillary carcinoma samples showed a higher expression of GLUT1 and GLUT3 in comparison with follicular carcinoma cases and non-neoplastic thyroid lesions. A tendency towards an increased expression of GLUT1 and GLUT3 was observed in papillary carcinoma cases with more advanced disease stages. Moreover, a significant correlation was noted between the hypoxia-related GLUT1 and GLUT3 expression determined by both methods. In conclusion, our findings suggest that GLUT1 and GLUT3 play an important role in the pathology of thyroid glands.
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Copy and paste a formatted citation
Spandidos Publications style
Jóźwiak P, Krześlak A, Pomorski L and Lipińska A: Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions. Mol Med Rep 6: 601-606, 2012.
APA
Jóźwiak, P., Krześlak, A., Pomorski, L., & Lipińska, A. (2012). Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions. Molecular Medicine Reports, 6, 601-606. https://doi.org/10.3892/mmr.2012.969
MLA
Jóźwiak, P., Krześlak, A., Pomorski, L., Lipińska, A."Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions". Molecular Medicine Reports 6.3 (2012): 601-606.
Chicago
Jóźwiak, P., Krześlak, A., Pomorski, L., Lipińska, A."Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions". Molecular Medicine Reports 6, no. 3 (2012): 601-606. https://doi.org/10.3892/mmr.2012.969
Copy and paste a formatted citation
x
Spandidos Publications style
Jóźwiak P, Krześlak A, Pomorski L and Lipińska A: Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions. Mol Med Rep 6: 601-606, 2012.
APA
Jóźwiak, P., Krześlak, A., Pomorski, L., & Lipińska, A. (2012). Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions. Molecular Medicine Reports, 6, 601-606. https://doi.org/10.3892/mmr.2012.969
MLA
Jóźwiak, P., Krześlak, A., Pomorski, L., Lipińska, A."Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions". Molecular Medicine Reports 6.3 (2012): 601-606.
Chicago
Jóźwiak, P., Krześlak, A., Pomorski, L., Lipińska, A."Expression of hypoxia-related glucose transporters GLUT1 and GLUT3 in benign, malignant and non-neoplastic thyroid lesions". Molecular Medicine Reports 6, no. 3 (2012): 601-606. https://doi.org/10.3892/mmr.2012.969
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