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Article

Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells

  • Authors:
    • Bin Yan
    • Qihong Fu
    • Lihua Lai
    • Xinran Tao
    • Yanghonghong  Fei
    • Jiangen Shen
    • Zuodong Chen
    • Qingqing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Ningbo First Hospital, Ningbo 315000, P.R. China, Department of Stomatology, Hangzhou Normal University Clinical Medicine College, Hangzhou 310036, P.R. China , Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, P.R. China
  • Pages: 675-681
    |
    Published online on: June 27, 2012
       https://doi.org/10.3892/mmr.2012.971
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Abstract

microRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. miRNAs play pivotal roles in physiological functioning and pathological progression. The function of microRNA-99a (miR-99a) in oral squamous cell carcinoma (OSCC) remains unclear. In the present study, we investigated the roles of miR-99a in OSCC development and the underlying mechanisms in 25 cases of primary OSCC tissues and Tca-8113 cells. The cells were analyzed using FACS analysis and western blotting. Results showed that the expression levels of miR-99a were markedly decreased in OSCC tissues compared with the adjacent non-tumor tissues (n=25). The results of in vitro experiments showed that miR-99a mimics significantly inhibited the proliferation of Tca-8113 cells, a tongue squamous carcinoma cell line, and that miR-99a mimics markedly induced the apoptosis of Tca-8113 cells. Furthermore, we demonstrated that mammalian target of rapamycin (mTOR) was directly targeted by miR-99a, as the overexpression of miR-99a in Tca-8113 cells downregulated the protein expression level of mTOR. Thus, our findings suggest that the downregulation of miR-99a in OSCC tissues is associated with tumor development. miR-99a regulates the growth and survival of OSCC cells and may be exploited as a biomarker and therapeutic target for patients with OSCC.
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1 

Bartel DP: MicroRNAs: target recognition and regulatory functions. Cell. 136:215–233. 2009.

2 

Xu P, Guo M and Hay BA: MicroRNAs and the regulation of cell death. Trends Genet. 20:617–624. 2004.

3 

Karp X and Ambros V: Developmental biology. Encountering microRNAs in cell fate signaling. Science. 310:1288–1289. 2005.

4 

Chen CZ, Li L, Lodish HF and Bartel DP: MicroRNAs modulate hematopoietic lineage differentiation. Science. 303:83–86. 2004.

5 

Poy MN, Eliasson L, Krutzfeldt J, et al: A pancreatic islet-specific microRNA regulates insulin secretion. Nature. 432:226–230. 2004.

6 

Garzon R, Fabbri M, Cimmino A, Calin GA and Croce CM: MicroRNA expression and function in cancer. Trends Mol Med. 12:580–587. 2006.

7 

Hermeking H: p53 enters the microRNA world. Cancer Cell. 12:414–418. 2007.

8 

Massano J, Regateiro FS, Januário G and Ferreira A: Oral squamous cell carcinoma: review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 102:67–76. 2006.

9 

Wu BH, Xiong XP, Jia J and Zhang WF: MicroRNAs: new actors in the oral cancer scene. Oral Oncol. 47:314–319. 2011.

10 

Li J, Huang H, Sun L, et al: MiR-21 indicates poor prognosis in tongue squamous cell carcinomas as an apoptosis inhibitor. Clin Cancer Res. 15:3998–4008. 2009.

11 

Wong TS, Liu XB, Wong BY, et al: Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res. 14:2588–2592. 2008.

12 

Wong TS, Liu XB, Chung-Wai Ho A, et al: Identification of pyruvate kinase type M2 as potential oncoprotein in squamous cell carcinoma of tongue through microRNA profiling. Int J Cancer. 123:251–257. 2008.

13 

Jiang L, Liu X, Kolokythas A, et al: Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma. Int J Cancer. 127:505–512. 2010.

14 

Liu Y, Chen Q, Song Y, et al: MicroRNA-98 negatively regulates IL-10 production and endotoxin tolerance in macrophages after LPS stimulation. FEBS Lett. 585:1963–1968. 2011.

15 

Wang QQ, Li H, Oliver T, et al: Integrin beta 1 regulates phagosome maturation in macrophages through Rac expression. J Immunol. 180:2419–2428. 2008.

16 

Yellen P, Saqcena M, Salloum D, et al: High-dose rapamycin induces apoptosis in human cancer cells by dissociating mTOR complex 1 and suppressing phosphorylation of 4E-BP1. Cell Cycle. 10:3948–3956. 2011.

17 

Chen CH and Sarbassov dos D: The mTOR (mammalian target of rapamycin) kinase maintains integrity of mTOR complex 2. J Biol Chem. 286:40386–40394. 2011.

18 

Liao YM, Kim C and Yen Y: Mammalian target of rapamycin and head and neck squamous cell carcinoma. Head Neck Oncol. 3:222011.

19 

Chang KY, Tsai SY, Wu CM, et al: Novel phosphoinositide 3-kinase/mTOR dual inhibitor, NVP-BGT226, displays potent growth-inhibitory activity against human head and neck cancer cells in vitro and in vivo. Clin Cancer Res. 17:7116–7126. 2011.

20 

Yang CJ, Shen WG, Liu CJ, et al: miR-221 and miR-222 expression increased the growth and tumorigenesis of oral carcinoma cells. J Oral Pathol Med. 40:560–566. 2011.

21 

Wiklund ED, Gao S, Hulf T, et al: MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma. PLoS One. 6:e278402011.

22 

Yang CC, Hung PS, Wang PW, et al: miR-181 as a putative biomarker for lymph-node metastasis of oral squamous cell carcinoma. J Oral Pathol Med. 40:397–404. 2011.

23 

Yu CC, Chen YW, Chiou GY, et al: MicroRNA let-7a represses chemoresistance and tumourigenicity in head and neck cancer via stem-like properties ablation. Oral Oncol. 47:202–210. 2011.

24 

Yu ZW, Zhong LP, Ji T, et al: MicroRNAs contribute to the chemoresistance of cisplatin in tongue squamous cell carcinoma lines. Oral Oncol. 46:317–322. 2010.

25 

Lo WL, Yu CC, Chiou GY, et al: MicroRNA-200c attenuates tumour growth and metastasis of presumptive head and neck squamous cell carcinoma stem cells. J Pathol. 223:482–495. 2010.

26 

Gomes CC and Gomez RS: MicroRNA and oral cancer: future perspectives. Oral Oncol. 44:910–914. 2008.

27 

Coutte L, Dreyer C, Sablin MP, Faivre S and Raymond E: PI3K-AKT-mTOR pathway and cancer. Bull Cancer. 99:173–180. 2012.(In French).

28 

Seeliger H, Guba M, Kleespies A, Jauch KW and Bruns CJ: Role of mTOR in solid tumor systems: a therapeutical target against primary tumor growth, metastases, and angiogenesis. Cancer Metastasis Rev. 26:611–621. 2007.

29 

Aissat N, Le Tourneau C, Ghoul A, et al: Antiproliferative effects of rapamycin as a single agent and in combination with carboplatin and paclitaxel in head and neck cancer cell lines. Cancer Chemother Pharmacol. 62:305–313. 2008.

30 

Fingar DC, Richardson CJ, Tee AR, et al: mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E. Mol Cell Biol. 24:200–216. 2004.

31 

Grewe M, Gansauge F, Schmid RM, Adler G and Seufferlein T: Regulation of cell growth and cyclin D1 expression by the constitutively active FRAP-p70s6K pathway in human pancreatic cancer cells. Cancer Res. 59:3581–3587. 1999.

32 

Sun D, Lee YS, Malhotra A, et al: miR-99 family of MicroRNAs suppresses the expression of prostate-specific antigen and prostate cancer cell proliferation. Cancer Res. 71:1313–1324. 2011.

33 

Li D, Liu X, Lin L, et al: MicroRNA-99a inhibits hepatocellular carcinoma growth and correlates with prognosis of patients with hepatocellular carcinoma. J Biol Chem. 286:36677–36685. 2011.

34 

Yamada H, Yanagisawa K, Tokumaru S, et al: Detailed characterization of a homozygously deleted region corresponding to a candidate tumor suppressor locus at 21q11-21 in human lung cancer. Genes Chromosomes Cancer. 47:810–818. 2008.

35 

Nam EJ, Yoon H, Kim SW, et al: MicroRNA expression profiles in serous ovarian carcinoma. Clin Cancer Res. 14:2690–2695. 2008.

36 

Catto JW, Miah S, Owen HC, et al: Distinct microRNA alterations characterize high- and low-grade bladder cancer. Cancer Res. 69:8472–8481. 2009.

37 

Hermeking H: The miR-34 family in cancer and apoptosis. Cell Death Differ. 17:193–199. 2010.

38 

He L, Thomson JM, Hemann MT, et al: A microRNA polycistron as a potential human oncogene. Nature. 435:828–833. 2005.

39 

Minor J, Wang X, Zhang F, et al: Methylation of microRNA-9 is a specific and sensitive biomarker for oral and oropharyngeal squamous cell carcinomas. Oral Oncol. 48:73–78. 2012.

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Copy and paste a formatted citation
Spandidos Publications style
Yan B, Fu Q, Lai L, Tao X, Fei Y, Shen J, Chen Z and Wang Q: Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells. Mol Med Rep 6: 675-681, 2012.
APA
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J. ... Wang, Q. (2012). Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells. Molecular Medicine Reports, 6, 675-681. https://doi.org/10.3892/mmr.2012.971
MLA
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J., Chen, Z., Wang, Q."Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells". Molecular Medicine Reports 6.3 (2012): 675-681.
Chicago
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J., Chen, Z., Wang, Q."Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells". Molecular Medicine Reports 6, no. 3 (2012): 675-681. https://doi.org/10.3892/mmr.2012.971
Copy and paste a formatted citation
x
Spandidos Publications style
Yan B, Fu Q, Lai L, Tao X, Fei Y, Shen J, Chen Z and Wang Q: Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells. Mol Med Rep 6: 675-681, 2012.
APA
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J. ... Wang, Q. (2012). Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells. Molecular Medicine Reports, 6, 675-681. https://doi.org/10.3892/mmr.2012.971
MLA
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J., Chen, Z., Wang, Q."Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells". Molecular Medicine Reports 6.3 (2012): 675-681.
Chicago
Yan, B., Fu, Q., Lai, L., Tao, X., Fei, Y., Shen, J., Chen, Z., Wang, Q."Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells". Molecular Medicine Reports 6, no. 3 (2012): 675-681. https://doi.org/10.3892/mmr.2012.971
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