Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus

  • Authors:
    • Xue Zhou
    • Fa-Huan Song
    • Wen He
    • Xiao-Yu Yang
    • Zhi-Bin Zhou
    • Xia Feng
    • Li-Hua Zhou
  • View Affiliations

  • Published online on: July 2, 2012     https://doi.org/10.3892/mmr.2012.976
  • Pages: 543-546
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Abstract

A growing number of studies have shown that commonly used anesthetic agents may cause neurohistopathological changes and persistent behavioral impairments in the developing brain. The effects of sevoflurane, a widely used substance in pediatric anesthesia, on the developing brain have not been thoroughly analyzed thus far. In this study, Sprague-Dawley rats at postnatal day (P)7 were exposed to 2.3% sevoflurane for 6 h continuously. Six hours after exposure, hematoxylin and eosin (H&E) staining was used to determine the morphological changes in the hippocampus. The expression levels of neuronal nitric oxide synthase (nNOS) and caspase-3 in the hippocampus of the pups were determined by immunofluorescence. A single 6-h sevoflurane exposure at P7 caused morphological changes in the hippocampus. Sevoflurane also significantly increased the expression of cleaved caspase-3 and reduced the expression of the nNOS protein. These results indicate that neonatal exposure to sevoflurane causes neurohistopathological changes, apoptosis and decreases nNOS protein levels in the rat hippocampus.
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September 2012
Volume 6 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., & Zhou, L. (2012). Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus. Molecular Medicine Reports, 6, 543-546. https://doi.org/10.3892/mmr.2012.976
MLA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6.3 (2012): 543-546.
Chicago
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6, no. 3 (2012): 543-546. https://doi.org/10.3892/mmr.2012.976