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September 2012 Volume 6 Issue 3

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Article

Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus

  • Authors:
    • Xue Zhou
    • Fa-Huan Song
    • Wen He
    • Xiao-Yu Yang
    • Zhi-Bin Zhou
    • Xia Feng
    • Li-Hua Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China , Department of Anatomy, Zhong Shan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 543-546
    |
    Published online on: July 2, 2012
       https://doi.org/10.3892/mmr.2012.976
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Abstract

A growing number of studies have shown that commonly used anesthetic agents may cause neurohistopathological changes and persistent behavioral impairments in the developing brain. The effects of sevoflurane, a widely used substance in pediatric anesthesia, on the developing brain have not been thoroughly analyzed thus far. In this study, Sprague-Dawley rats at postnatal day (P)7 were exposed to 2.3% sevoflurane for 6 h continuously. Six hours after exposure, hematoxylin and eosin (H&E) staining was used to determine the morphological changes in the hippocampus. The expression levels of neuronal nitric oxide synthase (nNOS) and caspase-3 in the hippocampus of the pups were determined by immunofluorescence. A single 6-h sevoflurane exposure at P7 caused morphological changes in the hippocampus. Sevoflurane also significantly increased the expression of cleaved caspase-3 and reduced the expression of the nNOS protein. These results indicate that neonatal exposure to sevoflurane causes neurohistopathological changes, apoptosis and decreases nNOS protein levels in the rat hippocampus.
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1 

Anand KJ: Anesthetic neurotoxicity in newborns: should we change clinical practice? Anesthesiology. 107:2–4. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Wiklund A, Granon S, Faure P, Sundman E, Changeux JP and Eriksson LI: Object memory in young and aged mice after sevoflurane anaesthesia. Neuroreport. 20:1419–1423. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Jevtovic-Todorovic V, Hartman RE, Izumi Y, et al: Early exposure to common anesthetic agents causes widespread neurodegeneration in the developing rat brain and persistent learning deficits. J Neurosci. 23:876–882. 2003.

4 

Perouansky M: General anesthetics and long-term neurotoxicity. Handb Exp Pharmacol. 182:143–157. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Lerman J, Sikich N, Kleinman S and Yentis S: The pharmacology of sevoflurane in infants and children. Anesthesiology. 80:814–824. 1994. View Article : Google Scholar : PubMed/NCBI

6 

Shelton KL: Discriminative stimulus effects of inhaled 1,1,1-trichloroethane in mice: comparison to other hydrocarbon vapors and volatile anesthetics. Psychopharmacology (Berl). 203:431–440. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Nishikawa K and Harrison NL: The actions of sevoflurane and desflurane on the gamma-aminobutyric acid receptor type A: effects of TM2 mutations in the alpha and beta subunits. Anesthesiology. 99:678–684. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Kvolik S, Dobrosevic B, Marczi S, Prlic L and Glavas-Obrovac L: Different apoptosis ratios and gene expressions in two human cell lines after sevoflurane anaesthesia. Acta Anaesthesiol Scand. 53:1192–1199. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Dong Y, Zhang G, Zhang B, Moir RD, Xia W, Marcantonio ER, Culley DJ, Crosby G, Tanzi RE and Xie Z: The common inhalational anesthetic sevoflurane induces apoptosis and increases beta-amyloid protein levels. Arch Neurol. 66:620–631. 2009.PubMed/NCBI

10 

Porro A, Chrochemore C, Cambuli F, Iraci N, Contestabile A and Perini G: Nitric oxide control of MYCN expression and multi drug resistance genes in tumours of neural origin. Curr Pharm Des. 16:431–439. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Hsu YY, Liu CM, Tsai HH, Jong YJ, Chen IJ and Lo YC: KMUP-1 attenuates serum deprivation-induced neurotoxicity in SH-SY5Y cells: roles of PKG, PI3K/Akt and Bcl-2/Bax pathways. Toxicology. 268:46–54. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Ciani E, Guidi S, Della Valle G, Perini G, Bartesaghi R and Contestabile A: Nitric oxide protects neuroblastoma cells from apoptosis induced by serum deprivation through cAMP-response element-binding protein (CREB) activation. J Biol Chem. 277:49896–49902. 2002. View Article : Google Scholar

13 

Iwase K, Takemura M, Shimada T, Wakisaka S, Nokubi T and Shigenaga Y: Ontogeny of NADPH-diaphorase in rat forebrain and midbrain. Anat Embryol (Berl). 197:229–247. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Porter AG and Janicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Woo M, Hakem R, Soengas MS, Duncan GS, Shahinian A, Kägi D, Hakem A, McCurrach M, Khoo W, Kaufman SA, et al: Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes. Genes Dev. 12:806–819. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Hirata H, Takahashi A, Kobayashi S, Yonehara S, Sawai H, Okazaki T, Yamamoto K and Sasada M: Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis. J Exp Med. 187:587–600. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Janicke RU, Sprengart ML, Wati MR and Porter AG: Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J Biol Chem. 273:9357–9360. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Satomoto M, Satoh Y, Terui K, Miyao H, Takishima K, Ito M and Imaki J: Neonatal exposure to sevoflurane induces abnormal social behaviors and deficits in fear conditioning in mice. Anesthesiology. 110:628–637. 2009. View Article : Google Scholar

19 

Win-Shwe TT, Yoshida Y, Kunugita N, Tsukahara S and Fujimaki H: Does early life toluene exposure alter the expression of NMDA receptor subunits and signal transduction pathway in infant mouse hippocampus? Neurotoxicology. 31:647–653. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Young C, Roth KA, Klocke BJ, West T, Holtzman DM, Labruyere J, Qin YQ, Dikranian K and Olney JW: Role of caspase-3 in ethanol-induced developmental neurodegeneration. Neurobiol Dis. 20:608–614. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Knott AB and Bossy-Wetzel E: Nitric oxide in health and disease of the nervous system. Antioxid Redox Signal. 11:541–554. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Garthwaite J: Concepts of neural nitric oxide-mediated transmission. Eur J Neurosci. 27:2783–2802. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou X, Song F, He W, Yang X, Zhou Z, Feng X and Zhou L: Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus. Mol Med Rep 6: 543-546, 2012.
APA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., & Zhou, L. (2012). Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus. Molecular Medicine Reports, 6, 543-546. https://doi.org/10.3892/mmr.2012.976
MLA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6.3 (2012): 543-546.
Chicago
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6, no. 3 (2012): 543-546. https://doi.org/10.3892/mmr.2012.976
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou X, Song F, He W, Yang X, Zhou Z, Feng X and Zhou L: Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus. Mol Med Rep 6: 543-546, 2012.
APA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., & Zhou, L. (2012). Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus. Molecular Medicine Reports, 6, 543-546. https://doi.org/10.3892/mmr.2012.976
MLA
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6.3 (2012): 543-546.
Chicago
Zhou, X., Song, F., He, W., Yang, X., Zhou, Z., Feng, X., Zhou, L."Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus". Molecular Medicine Reports 6, no. 3 (2012): 543-546. https://doi.org/10.3892/mmr.2012.976
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