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Article

Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles

  • Authors:
    • Yan-Long Bi
    • Ming-Feng Wu
    • Li-Xia Lu
    • Qi Zhou
    • Fei Du
    • Xiao-Ting Sun
    • Sen-Fei Tang
    • Guo-Tong Xu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Tongji Hospital Affiliated to Tongji University School of Medicine, Shanghai 200065, P.R. China, Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200092, P.R. China
  • Pages: 1767-1772
    |
    Published online on: April 10, 2013
       https://doi.org/10.3892/mmr.2013.1418
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Abstract

To avoid donor tissue shortages, ex vivo cultured human corneal endothelial cell (HCEC) transplantation is a promising therapeutic resource. Superparamagnetic iron oxide nanoparticle (SPION) cell labeling assists HCEC transplantation by attaching the posterior corneal stroma in ex vivo animal models. However, possible functional changes of the HCECs following SPION labeling remain to be determined. In this study, we used SPIONs to label cultured rabbit CECs (RCECs) in order to observe important cell functions and the levels of cell markers. The synthetic SPIONs exhibited superparamagnetism at room temperature, with saturation magnetization of 55.4 emu/g and negligible remanence or coercivity. The ζ-potential was -24.5 mV and the diameter was 101±55 nm. Immunostaining demonstrated a normal density of zonula occluden-1 (ZO-1), nestin and Ki-67 at cellular junctions or in nuclei from RCECs following SPION labeling at 16 µg/ml. MTT cytotoxicity assay, homotypic adhesion assay, quantitative flow cytometric Ki-67 analysis and RCEC pump function measurement demonstrated no significant differences between the cells with or without SPION labeling (P<0.05, for all assays). Results of this study demonstrated successful labeled cultured RCECs with synthetic SPIONs. Labeled cells possessed several important characteristics required to maintain the transparency and refractive parameters of the cornea, including hexagonal cell morphology, higher cell adhesion ability and proliferative potential, cell pump function and the positive expression of several cell markers.
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Copy and paste a formatted citation
Spandidos Publications style
Bi Y, Wu M, Lu L, Zhou Q, Du F, Sun X, Tang S and Xu G: Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles. Mol Med Rep 7: 1767-1772, 2013.
APA
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X. ... Xu, G. (2013). Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles. Molecular Medicine Reports, 7, 1767-1772. https://doi.org/10.3892/mmr.2013.1418
MLA
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X., Tang, S., Xu, G."Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles". Molecular Medicine Reports 7.6 (2013): 1767-1772.
Chicago
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X., Tang, S., Xu, G."Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles". Molecular Medicine Reports 7, no. 6 (2013): 1767-1772. https://doi.org/10.3892/mmr.2013.1418
Copy and paste a formatted citation
x
Spandidos Publications style
Bi Y, Wu M, Lu L, Zhou Q, Du F, Sun X, Tang S and Xu G: Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles. Mol Med Rep 7: 1767-1772, 2013.
APA
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X. ... Xu, G. (2013). Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles. Molecular Medicine Reports, 7, 1767-1772. https://doi.org/10.3892/mmr.2013.1418
MLA
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X., Tang, S., Xu, G."Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles". Molecular Medicine Reports 7.6 (2013): 1767-1772.
Chicago
Bi, Y., Wu, M., Lu, L., Zhou, Q., Du, F., Sun, X., Tang, S., Xu, G."Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles". Molecular Medicine Reports 7, no. 6 (2013): 1767-1772. https://doi.org/10.3892/mmr.2013.1418
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