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Article

Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells

  • Authors:
    • Zhiqiang Li
    • Xiaojun Tian
    • Yan Yuan
    • Zhixiu Song
    • Lili Zhang
    • Xia Wang
    • Tong Li
  • View Affiliations / Copyright

    Affiliations: Department of Integrated Traditional Chinese and Western Medicine, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Tuberculosis, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
  • Pages: 1945-1949
    |
    Published online on: April 10, 2013
       https://doi.org/10.3892/mmr.2013.1423
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Abstract

Mesenchymal stem cell (MSC) therapy is a promising treatment for diseases of the nervous system. However, MSCs often lose their stemness and homing abilities when cultured in conventional two‑dimensional (2D) systems. Consequently, it is important to explore novel culture methods for MSC-based therapies in clinical practice. To investigate the effect of a cell culture using chitosan membranes on MSCs, the morphology of MSCs cultured using chitosan membranes was observed and the expression of stemness marker genes was analyzed. We demonstrated that MSCs cultured using chitosan membranes form spheroids. Additionally, the expression of stemness marker genes, including Oct4, Sox2 and Nanog, increased significantly when MSCs were cultured using chitosan membranes compared with 2D culture systems. Finally, MSCs cultured using chitosan membranes were found to have an increased potential to differentiate into nerve cells and chrondrocytes. In conclusion, we demonstrated that MSCs cultured on chitosan membranes maintain their stemness and homing abilities. This finding may be further investigated for the development of novel cell-based therapies for diseases involving neuron-like cells and chondrogenesis.
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1 

Phinney DG and Prockop DJ: Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair - current views. Stem Cells. 25:2896–2902. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Pittenger MF, Mackay AM, Beck SC, et al: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Pittenger MF and Martin BJ: Mesenchymal stem cells and their potential as cardiac therapeutics. Circ Res. 95:9–20. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues. Science. 276:71–74. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Wu Y, Chen L, Scott PG and Tredget EE: Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis. Stem Cells. 25:2648–2659. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Wu Y, Zhao RC and Tredget EE: Concise review: bone marrow-derived stem/progenitor cells in cutaneous repair and regeneration. Stem Cells. 28:905–915. 2010.PubMed/NCBI

7 

Chen L, Tredget EE, Wu PY and Wu Y: Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS One. 3:e18862008. View Article : Google Scholar : PubMed/NCBI

8 

Nagai A, Kim WK, Lee HJ, et al: Multilineage potential of stable human mesenchymal stem cell line derived from fetal marrow. PLoS One. 2:e12722007. View Article : Google Scholar : PubMed/NCBI

9 

Aggarwal S and Pittenger MF: Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 105:1815–1822. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Jiang XX, Zhang Y, Liu B, Zhang SX, Wu Y, Yu XD and Mao N: Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells. Blood. 105:4120–4126. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Baxter MA, Wynn RF, Jowitt SN, Wraith JE, Fairbairn LJ and Bellantuono I: Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells. 22:675–682. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Krampera M, Pasini A, Rigo A, et al: HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells: inducing cell expansion and reversibly preventing multilineage differentiation. Blood. 106:59–66. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Wagner W, Horn P, Castoldi M, et al: Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS One. 3:e22132008. View Article : Google Scholar : PubMed/NCBI

14 

Pelttari K, Winter A, Steck E, et al: Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice. Arthritis Rheum. 54:3254–3266. 2006. View Article : Google Scholar

15 

Crisostomo PR, Wang M, Wairiuko GM, Morrell ED, Terrell AM, Seshadri P, Nam UH and Meldrum DR: High passage number of stem cells adversely affects stem cell activation and myocardial protection. Shock. 26:575–580. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Lin SJ, Jee SH, Hsaio WC, Lee SJ and Young TH: Formation of melanocyte spheroids on the chitosan-coated surface. Biomaterials. 26:1413–1422. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Yang TL and Young TH: The specificity of chitosan in promoting branching morphogenesis of progenitor salivary tissue. Biochem Biophys Res Commun. 381:466–470. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Yeh LK, Chen YH, Chiu CS, Hu FR, Young TH and Wang IJ: The phenotype of bovine corneal epithelial cells on chitosan membrane. J Biomed Mater Res A. 90:18–26. 2009.PubMed/NCBI

19 

Chatelet C, Damour O and Domard A: Influence of the degree of acetylation on some biological properties of chitosan films. Biomaterials. 22:261–268. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Chen YH, Wang IJ and Young TH: Formation of keratocyte spheroids on chitosan-coated surface can maintain keratocyte phenotypes. Tissue Eng Part A. 15:2001–2013. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Chiao E, Kmet M, Behr B and Baker J: Derivation of human embryonic stem cells in standard and chemically defined conditions. Methods Cell Biol. 86:1–14. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Richards M, Fong CY, Chan WK, Wong PC and Bongso A: Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol. 20:933–936. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Lee ST, Yun JI, Jo YS, et al: Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche. Biomaterials. 31:1219–1226. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Brafman DA, Chang CW, Fernandez A, Willert K, Varghese S and Chien S: Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces. Biomaterials. 31:9135–9144. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Nur-E-Kamal A, Ahmed I, Kamal J, Schindler M and Meiners S: Three-dimensional nanofibrillar surfaces promote self-renewal in mouse embryonic stem cells. Stem Cells. 24:426–433. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Gerecht S, Burdick JA, Ferreira LS, Townsend SA, Langer R and Vunjak-Novakovic G: Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells. Proc Natl Acad Sci USA. 104:11298–11303. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Abraham S, Eroshenko N and Rao RR: Role of bioinspired polymers in determination of pluripotent stem cell fate. Regen Med. 4:561–578. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Kopp JL, Ormsbee BD, Desler M and Rizzino A: Small increases in the level of Sox2 trigger the differentiation of mouse embryonic stem cells. Stem Cells. 26:903–911. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Sokolov MV, Panyutin IV, Onyshchenko MI, Panyutin IG and Neumann RD: Expression of pluripotency-associated genes in the surviving fraction of cultured human embryonic stem cells is not significantly affected by ionizing radiation. Gene. 455:8–15. 2010. View Article : Google Scholar

30 

Baer PC, Griesche N, Luttmann W, Schubert R, Luttmann A and Geiger H: Human adipose-derived mesenchymal stem cells in vitro: evaluation of an optimal expansion medium preserving stemness. Cytotherapy. 12:96–106. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Fortunel NO, Otu HH, Ng HH, et al: Comment on ‘Stemness: transcriptional profiling of embryonic and adult stem cells’ and ‘a stem cell molecular signature’. Science. 302:3932003.

32 

Kim SY and Im GI: Control of the chondrogenesis during the development and its application for cartilage tissue engineering. J Tissue Eng Regen Med. 6:595–602. 2009.

33 

Bartosh TJ, Ylöstalo JH, Mohammadipoor A, et al: Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci USA. 107:13724–13729. 2010. View Article : Google Scholar

34 

Wang W, Itaka K, Ohba S, Nishiyama N, Chung UI, Yamasaki Y and Kataoka K: 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials. 30:2705–2715. 2009. View Article : Google Scholar

35 

Miyagawa Y, Okita H, Hiroyama M, et al: A microfabricated scaffold induces the spheroid formation of human bone marrow-derived mesenchymal progenitor cells and promotes efficient adipogenic differentiation. Tissue Eng Part A. 17:513–521. 2011. View Article : Google Scholar

36 

Quintana L, zur Nieden NI and Semino CE: Morphogenetic and regulatory mechanisms during developmental chondrogenesis: new paradigms for cartilage tissue engineering. Tissue Eng Part B Rev. 15:29–41. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Chang JC, Hsu SH and Chen DC: The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture. Biomaterials. 30:6265–6275. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Martinez-Diaz S, Garcia-Giralt N, Lebourg M, et al: In vivo evaluation of 3-dimensional polycaprolactone scaffolds for cartilage repair in rabbits. Am J Sports Med. 38:509–519. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Tian X, Yuan Y, Song Z, Zhang L, Wang X and Li T: Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells. Mol Med Rep 7: 1945-1949, 2013.
APA
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., & Li, T. (2013). Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells. Molecular Medicine Reports, 7, 1945-1949. https://doi.org/10.3892/mmr.2013.1423
MLA
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., Li, T."Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells". Molecular Medicine Reports 7.6 (2013): 1945-1949.
Chicago
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., Li, T."Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells". Molecular Medicine Reports 7, no. 6 (2013): 1945-1949. https://doi.org/10.3892/mmr.2013.1423
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z, Tian X, Yuan Y, Song Z, Zhang L, Wang X and Li T: Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells. Mol Med Rep 7: 1945-1949, 2013.
APA
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., & Li, T. (2013). Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells. Molecular Medicine Reports, 7, 1945-1949. https://doi.org/10.3892/mmr.2013.1423
MLA
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., Li, T."Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells". Molecular Medicine Reports 7.6 (2013): 1945-1949.
Chicago
Li, Z., Tian, X., Yuan, Y., Song, Z., Zhang, L., Wang, X., Li, T."Effect of cell culture using chitosan membranes on stemness marker genes in mesenchymal stem cells". Molecular Medicine Reports 7, no. 6 (2013): 1945-1949. https://doi.org/10.3892/mmr.2013.1423
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