Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs

Jin,Ji-Chun; Jin,Xing-Lin; Zhang,Xian; Piao,Ying-Shi; Liu,Shuang-Ping

doi:10.3892/mmr.2013.1428

Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs

Authors:
- Ji-Chun Jin
- Xing-Lin Jin
- Xian Zhang
- Ying-Shi Piao
- Shuang-Ping Liu
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Affiliations: Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China, Department of Pathophysiology, Yanbian University Medical College, Yanji, Jilin 133000, P.R. China, Cancer Research Center, Yanbian University, Yanji, Jilin 133000, P.R. China
Published online on: April 11, 2013 https://doi.org/10.3892/mmr.2013.1428
Pages: 1831-1837

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Abstract

3β,16β,17α-trihydroxycholest-5-en-22-one 16-O-(2-O-4-methoxybenzoyl-β-D-xylopyranosyl)-(1→3)- (2-O-acetyl-α-L-arabinopyranoside) (OSW-1) is a member of the cholestane saponin family, which was first isolated from the bulbs of Ornithogalum saundersiae and previously reported to be cytotoxic against several types of malignant cells. However, its antitumor mechanism remains unclear. Therefore, we investigated microRNA (miRNA) expression profiles in order to explore the antitumor activities of OSW-1. Furthermore, following study of differentially expressed miRNAs, the function of novel miRNAs and OSW-1 was determined using known miRNAs and anticarcinogens. The present study demonstrated that treatment with OSW-1 leads to the upregulation and downregulation of a large set of tumor-related miRNAs, including miR-299, miR-1908, miR-125b, miR-187a, miR-1275, hav1-miR-H6-3p, miR-181, miR-210, miR-483, miR-126, miR-208 and others. Notably, miR-141, miR-142, miR-200C and miR-1275 were found to be upregulated by OSW-1 and doxorubicine, as compared with doxorubicine alone. Additionally, the expression fold-change of miR-142-3P was ~58 times higher than its expression with a different treatment. These miRNAs are linked to cancer, including proliferation, differentiation, apoptosis, cell adhesion, migration, polarity and epithelial to mesenchymal transition (EMT).

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