Role of PTEN in cholera toxin-induced SWO‑38 glioma cell differentiation

Wang,Ming  Hua; Lin,Chen  Li; Zhang,Ji  Jun; Weng,Ze  Ping; Hu,Ting; Xie,Qiang; Zhong,Xue  Yun

doi:10.3892/mmr.2013.1434

June 2013 Volume 7 Issue 6

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June 2013 Volume 7 Issue 6

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Article

Role of PTEN in cholera toxin-induced SWO‑38 glioma cell differentiation

Authors:
- Ming Hua Wang
- Chen Li Lin
- Ji Jun Zhang
- Ze Ping Weng
- Ting Hu
- Qiang Xie
- Xue Yun Zhong
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Affiliations: Department of Pathology, Hainan Medical College, Haikou, Hainan 571101, P.R. China, Department of Pathology, Medical College of Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Medical Oncology, Guangzhou Armed Police Force Hospital, Guangzhou, Guangdong 510632, P.R. China
Pages: 1912-1918
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Published online on: April 18, 2013

https://doi.org/10.3892/mmr.2013.1434
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Abstract

Malignant gliomas persist as a major disease responsible for high morbidity and mortality rates in adults. Differentiation therapy has emerged as a promising treatment modality. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene function is commonly lost in primary gliomas, particularly in glioblastomas, and this is associated with tumor differentiation. PTEN gene deletion is one of the main molecular events in gliomas. In this study, we aimed to explore the effect and mechanisms of PTEN on cholera toxin (CT)‑induced SWO-38 glioma cell differentiation. It has been shown that transfection of the exogenous PTEN gene induces glioma cell differentiation; however, the underlying mechanism remains to be elucidated. Results of the present study showed that CT-induced SWO-38 glioma cell differentiation was characterized by morphological changes, the increased expression of glial fibrillary acidic protein (GFAP), an accumulation of cells in the G0/G1 phase of the cell cycle, the decreased expression of cyclin D1 and a decreased invasion and migration capacity. Silencing of the PTEN protein using RNA interference resulted in suppressed cell differentiation. Furthermore, inhibition of the PI3K/AKT pathway by the inhibitor LY294002 led to attenuated differentiation, while differentiation remained stable with the inhibition of the MAPK/ERK pathway by PD0325901. Thus, PTEN may be important in glioma cell differentiation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Role of PTEN in cholera toxin-induced SWO‑38 glioma cell differentiation

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