Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June 2013 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3

  • Authors:
    • Fengsheng Li
    • Ling Gao
    • Qisheng Jiang
    • Zhidong Wang
    • Bo Dong
    • Tao Yan
    • Xiaohua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Therapy of ARS, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China, Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, P.R. China, The Second Artillery General Hospital, Beijing 100088, P.R. China
  • Pages: 1883-1888
    |
    Published online on: April 25, 2013
       https://doi.org/10.3892/mmr.2013.1441
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the effect of radiation on the invasion of the pulmonary adenocarcinoma cell line, A549, was investigated. Invasion of A549 cells irradiated with 2 and 4 Gy doses of γ‑ray was detected using the transwell Matrigel invasion assay. Levels of matrix metalloproteinase 2 (MMP‑2) and phosphorylated signal transducer and activator of transcription 3 (STAT3) were detected by reverse transcription PCR (RT-PCR) and/or immunoblotting. The enzyme activity of MMP‑2 was examined by gelatin zymography. Results demonstrated that the invasion of A549 cells was significantly enhanced by γ‑ray radiation at doses of 2 or 4 Gy. In addition, exposure to radiation was found to promote transcriptional expression of MMP‑2 and increase MMP‑2 enzyme activity. Irradiation activated the phosphorylation of STAT3 and promoted the nuclear localization of STAT3. The blockage of STAT3 phosphorylation using a specific inhibitor (AG490) suppressed the irradiation‑induced elevation of MMP‑2 expression, enzyme activity and invasion of A549 cells. Finally, the expression of vascular endothelial growth factor (VEGF) was found to be upregulated by radiation, which was associated with the activation of STAT3. Results of the current study indicate that irradiation leads to activation of STAT3 and translocation to the nucleus, leading to activation of VEGF and MMP‑2 transcription, resulting in the increased invasion of A549 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Klepper L: An interactive method for optimization of irradiation plans in radiation therapy of malignant tumors. Med Tekh. 25–30. 2006.(In Russian).

2 

Rastogi S, Boylan M, Wright EG and Coates PJ: Interactions of apoptotic cells with macrophages in radiation-induced bystander signaling. Radiat Res. 179:135–145. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Sun Y and Nelson PS: Molecular pathways: involving microenvironment damage responses in cancer therapy resistance. Clin Cancer Res. 18:4019–4025. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Madani I, De Neve W and Mareel M: Does ionizing radiation stimulate cancer invasion and metastasis? Bull Cancer. 95:292–300. 2008.PubMed/NCBI

5 

Kaur H, Buettner H, Salomao DR and Marks RS: Transcleral orbital invasion by a radiation and chemotherapy-resistant choroidal metastasis of a pulmonary adenocarcinoma. Am J Ophthalmol. 143:369–370. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Itoh YH, Nakatsugawa S, Oguri T and Miyata N: Increase of cellular fibrinolysis in human lung cancer cell line by radiation: relationship between urokinase-type plasminogen activator (uPA) and metastasis and invasion. Nihon Igaku Hoshasen Gakkai Zasshi. 56:747–749. 1996.(In Japanese).

7 

Boutrus R, Abi-Raad R, Niemierko A, et al: Does lymphovascular invasion predict regional nodal failure in breast cancer patients with zero to three positive lymph nodes treated with conserving surgery and radiotherapy? Implications for regional radiation. Int J Radiat Oncol Biol Phys. 78:793–798. 2010. View Article : Google Scholar

8 

Guerra LE, Smith RM, Kaminski A, Lagios MD and Silverstein MJ: Invasive local recurrence increased after radiation therapy for ductal carcinoma in situ. Am J Surg. 196:552–555. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Fidler IJ, Kim SJ and Langley RR: The role of the organ microenvironment in the biology and therapy of cancer metastasis. J Cell Biochem. 101:927–936. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Kargozaran H, Yuan SY, Breslin JW, et al: A role for endothelial-derived matrix metalloproteinase-2 in breast cancer cell transmigration across the endothelial-basement membrane barrier. Clin Exp Metastasis. 24:495–502. 2007. View Article : Google Scholar

11 

Kerkelä E and Saarialho-Kere U: Matrix metalloproteinases in tumor progression: focus on basal and squamous cell skin cancer. Exp Dermatol. 12:109–125. 2003.PubMed/NCBI

12 

Stetler-Stevenson WG: The role of matrix metalloproteinases in tumor invasion, metastasis, and angiogenesis. Surg Oncol Clin N Am. 10:383–392. 2001.PubMed/NCBI

13 

Araya J, Maruyama M, Sassa K, et al: Ionizing radiation enhances matrix metalloproteinase-2 production in human lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 280:L30–L38. 2001.PubMed/NCBI

14 

Sawaya R, Tofilon PJ, Mohanam S, et al: Induction of tissue-type plasminogen activator and 72-kDa type-IV collagenase by ionizing radiation in rat astrocytes. Int J Cancer. 56:214–218. 1994. View Article : Google Scholar : PubMed/NCBI

15 

Zhao W, O’Malley Y and Robbins ME: Irradiation of rat mesangial cells alters the expression of gene products associated with the development of renal fibrosis. Radiat Res. 152:160–169. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Zhao W, O’Malley Y, Wei S and Robbins ME: Irradiation of rat tubule epithelial cells alters the expression of gene products associated with the synthesis and degradation of extracellular matrix. Int J Radiat Biol. 76:391–402. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Lui VW, Boehm AL, Koppikar P, et al: Antiproliferative mechanisms of a transcription factor decoy targeting signal transducer and activator of transcription (STAT) 3: the role of STAT1. Mol Pharmacol. 71:1435–1443. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Ju H, Venema VJ, Liang H, Harris MB, Zou R and Venema RC: Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257–264. 2000. View Article : Google Scholar

19 

Shen Y, Devgan G, Darnell JE Jr and Bromberg JF: Constitutively activated Stat3 protects fibroblasts from serum withdrawal and UV-induced apoptosis and antagonizes the proapoptotic effects of activated Stat1. Proc Natl Acad Sci USA. 98:1543–1548. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Li Q, Wang Y, Xie C, Qiu X and Wang E: Expression of MMP-2, MMP-9, TIMP-1 and their relationship with prognosis in NSCLC. Zhongguo Fei Ai Za Zhi. 7:497–500. 2004.(In Chinese).

21 

Gariboldi MB, Ravizza R, Molteni R, Osella D, Gabano E and Monti E: Inhibition of Stat3 increases doxorubicin sensitivity in a human metastatic breast cancer cell line. Cancer Lett. 258:181–188. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Jeanes A, Gottardi CJ and Yap AS: Cadherins and cancer: how does cadherin dysfunction promote tumor progression? Oncogene. 27:6920–6929. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Tsutsumi K, Tsuda M, Yazawa N, et al: Increased motility and invasiveness in tumor cells that survive 10 Gy irradiation. Cell Struct Funct. 34:89–96. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Camphausen K, Moses MA, Beecken WD, Khan MK, Folkman J and O’Reilly MS: Radiation therapy to a primary tumor accelerates metastatic growth in mice. Cancer Res. 61:2207–2211. 2001.PubMed/NCBI

25 

Zhang W, Nwagwu C, Le DM, Yong VW, Song H and Couldwell WT: Increased invasive capacity of connexin43-overexpressing malignant glioma cells. J Neurosurg. 99:1039–1046. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Mayo JG: Biologic characterization of the subcutaneously implanted Lewis lung tumor. Cancer Chemother Rep 2. 3:325–330. 1972.PubMed/NCBI

27 

Jung JW, Hwang SY, Hwang JS, Oh ES, Park S and Han IO: Ionising radiation induces changes associated with epithelial-mesenchymal transdifferentiation and increased cell motility of A549 lung epithelial cells. Eur J Cancer. 43:1214–1224. 2007. View Article : Google Scholar

28 

Béliveau A, Bérubé M, Rousseau A, Pelletier G and Guérin SL: Expression of integrin alpha5beta1 and MMPs associated with epithelioid morphology and malignancy of uveal melanoma. Invest Ophthalmol Vis Sci. 41:2363–2372. 2000.PubMed/NCBI

29 

Cheng JC, Chou CH, Kuo ML and Hsieh CY: Radiation-enhanced hepatocellular carcinoma cell invasion with MMP-9 expression through PI3K/Akt/NF-kappaB signal transduction pathway. Oncogene. 25:7009–7018. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Park CM, Park MJ, Kwak HJ, et al: Ionizing radiation enhances matrix metalloproteinase-2 secretion and invasion of glioma cells through Src/epidermal growth factor receptor-mediated p38/Akt and phosphatidylinositol 3-kinase/Akt signaling pathways. Cancer Res. 66:8511–8519. 2006. View Article : Google Scholar

31 

Radisky ES and Radisky DC: Matrix metalloproteinase-induced epithelial-mesenchymal transition in breast cancer. J Mammary Gland Biol Neoplasia. 15:201–212. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Devarajan E and Huang S: STAT3 as a central regulator of tumor metastases. Curr Mol Med. 9:626–633. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Singh-Gupta V, Zhang H, Banerjee S, et al: Radiation-induced HIF-1alpha cell survival pathway is inhibited by soy isoflavones in prostate cancer cells. Int J Cancer. 124:1675–1684. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Abdulghani J, Gu L, Dagvadorj A, et al: Stat3 promotes metastatic progression of prostate cancer. Am J Pathol. 172:1717–1728. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Lynch DH, Gurish MF and Daynes RA: The effects of ultraviolet irradiation on the generation of anti-tumor cytotoxic effector cell responses in vitro. J Immunol. 127:1163–1168. 1981.PubMed/NCBI

36 

Frey B, Rubner Y, Wunderlich R, et al: Induction of abscopal anti-tumor immunity and immunogenic tumor cell death by ionizing irradiation - implications for cancer therapies. Curr Med Chem. 19:1751–1764. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Jürgenliemk-Schulz IM, Renes IB, Rutgers DH, et al: Anti-tumor effects of local irradiation in combination with peritumoral administration of low doses of recombinant interleukin-2 (rIL-2). Radiat Oncol Investig. 5:54–61. 1997.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li F, Gao L, Jiang Q, Wang Z, Dong B, Yan T and Chen X: Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Mol Med Rep 7: 1883-1888, 2013.
APA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., & Chen, X. (2013). Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Molecular Medicine Reports, 7, 1883-1888. https://doi.org/10.3892/mmr.2013.1441
MLA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7.6 (2013): 1883-1888.
Chicago
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7, no. 6 (2013): 1883-1888. https://doi.org/10.3892/mmr.2013.1441
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Gao L, Jiang Q, Wang Z, Dong B, Yan T and Chen X: Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Mol Med Rep 7: 1883-1888, 2013.
APA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., & Chen, X. (2013). Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3. Molecular Medicine Reports, 7, 1883-1888. https://doi.org/10.3892/mmr.2013.1441
MLA
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7.6 (2013): 1883-1888.
Chicago
Li, F., Gao, L., Jiang, Q., Wang, Z., Dong, B., Yan, T., Chen, X."Radiation enhances the invasion abilities of pulmonary adenocarcinoma cells via STAT3". Molecular Medicine Reports 7, no. 6 (2013): 1883-1888. https://doi.org/10.3892/mmr.2013.1441
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team