Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June 2013 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of fumonisin B1 on the cell cycle of normal human liver cells

  • Authors:
    • Shao-Kang Wang
    • Sha Liu
    • Li-Gang Yang
    • Ruo-Fu Shi
    • Gui-Ju Sun
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 1970-1976
    |
    Published online on: April 26, 2013
       https://doi.org/10.3892/mmr.2013.1447
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Fumonisin B1 (FB1) is a well-known liver and kidney carcinogen in rodents and humans. The aim of the present study was to investigate the effect of FB1 on the proliferation and cell cycle of the normal human liver cell line HL-7702 and to explore the underlying molecular mechanisms of action. The cells were treated with FB1 (0.0, 0.1, 1.0, 10.0 and 100.0 µmol/l) for 24, 48, 72 and 96 h. Cell proliferation was assessed by colorimetric assay. Cell cycle analysis was performed by flow cytometry. The mRNA and protein expression of cyclin E and P21 were determined by RT‑PCR and western blot analysis, respectively. FB1 was initially demonstrated to significantly inhibit the proliferation of HL-7702 cells; however, cell proliferation increased with increasing treatment time. The percentage of cells in the G0/G1 phase was significantly increased by FB1; however, significantly decreased with an increasing concentration of FB1. The mRNA expression of cyclin E was upregulated and then gradually downregulated with increasing treatment time. The mRNA expression of P21 was significantly increased following treatment with 0.1 µmol/l FB1, and decreased following treatment with 10.0 and 100.0 µmol/l FB1 for different treatment durations. Western blot analysis showed that FB1 significantly increased the protein expression of cyclin E and significantly decreased the protein expression of P21 at various concentrations and treatment durations. Our results demonstrated that FB1 affects the cell cycle of normal human liver cells and that the underlying mechanism of action is associated with alterations in the expression levels of cyclin E and P21 induced by FB1.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Theumer MG, López AG, Aoki MP, et al: Subchronic mycotoxicoses in rats. Histopathological changes and modulation of the sphinganine to sphingosine (Sa/So) ratio imbalance induced by Fusarium verticillioides culture material, due to the coexistence of aflatoxin B1 in the diet. Food Chem Toxicol. 46:967–977. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Stockmann-Juvala H and Savolainen K: A review of the toxic effects and mechanisms of action of fumonisin B1. Hum Exp Toxicol. 27:799–809. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Cano-Sancho G, Ramos AJ, Marín S and Sanchis V: Occurrence of fumonisins in Catalonia (Spain) and an exposure assessment of specific population groups. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 29:799–808. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Ledoux DR, Broomhead JN, Bermudez AJ and Rottinghaus GE: Individual and combined effects of the Fusarium mycotoxins fumonisin B1 and moniliformin in broiler chicks. Avian Dis. 47:1368–1375. 2003.PubMed/NCBI

5 

Gelderblom WC, Marasas WF, Lebepe-Mazur S, et al: Cancer initiating properties of fumonisin B1 in a short-term rat liver carcinogenesis assay. Toxicology. 250:89–95. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Y, Jones C and Dickman MB: Identification of differentially expressed genes following treatment of monkey kidney cells with the mycotoxin fumonisin B(1). Food Chem Toxicol. 39:45–53. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Wattenberg EV, Badria FA and Shier WT: Activation of mitogen-activated protein kinase by the carcinogenic mycotoxin fumonisin B1. Biochem Biophys Res Commun. 227:622–627. 1996. View Article : Google Scholar : PubMed/NCBI

8 

Bouhet S, Hourcade E, Loiseau N, et al: The mycotoxin fumonisin B1 alters the proliferation and the barrier function of porcine intestinal epithelial cells. Toxicol Sci. 77:165–171. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Theumer MG, Cánepa MC, López AG, et al: Subchronic mycotoxicoses in Wistar rats: assessment of the in vivo and in vitro genotoxicity induced by fumonisins and aflatoxin B(1), and oxidative stress biomarkers status. Toxicology. 268:104–110. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Rumora L, Kovacić S, Rozgaj R, et al: Cytotoxic and genotoxic effects of fumonisin B1 on rabbit kidney RK13 cell line. Arch Toxicol. 76:55–61. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Sharma N, He Q and Sharma RP: Sphingosine kinase activity confers resistance to apoptosis by fumonisin B1 in human embryonic kidney (HEK-293) cells. Chem Biol Interact. 151:33–42. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Rentz SS, Showker JL, Meredith FI, et al: Inhibition of sphingolipid biosynthesis decreases phosphorylated ERK2 in LLC-PK1 cells. Food Chem Toxicol. 43:123–131. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Galvano F, Campisi A, Russo A, et al: DNA damage in astrocytes exposed to fumonisin B1. Neurochem Res. 27:345–351. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Ribeiro DH, Ferreira FL, da Silva VN, et al: Effects of aflatoxin b(1) and fumonisin b(1) on the viability and induction of apoptosis in rat primary hepatocytes. Int J Mol Sci. 11:1944–1955. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Schwerdt G, Konigs M, Holzinger H, et al: Effects of the mycotoxin fumonisin B(1) on cell death in human kidney cells and human lung fibroblasts in primary culture. J Appl Toxicol. 29:174–182. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Galvano F, Russo A, Cardile V, et al: DNA damage in human fibroblasts exposed to fumonisin B1. Food Chem Toxicol. 40:25–31. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Seefelder W, Humpf HU, Schwerdt G, et al: Induction of apoptosis in cultured human proximal tubule cells by fumonisins and fumonisin metabolites. Toxicol Appl Pharmacol. 192:146–153. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Kouadio JH, Mobio TA, Baudrimont I, et al: Comparative study of cytotoxicity and oxidative stress induced by deoxynivalenol, zearalenone or fumonisin B1 in human intestinal cell line Caco-2. Toxicology. 213:56–65. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Czerednik A, Busscher M, Bielen BA, et al: Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes. J Exp Bot. 63:2605–2617. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Yamamoto S, Kohsaka S and Nakajima K: Role of cell cycle-associated proteins in microglial proliferation in the axotomized rat facial nucleus. Glia. 60:570–581. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Li J, Deng M, Wei Q, et al: Phosphorylation of MCM3 protein by cyclin E/cyclin-dependent kinase 2 (Cdk2) regulates its function in cell cycle. J Biol Chem. 286:39776–39785. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Roy HK, Koetsier JL, Tiwari AK, et al: Involvement of p21cip1/waf1 in the anti-proliferative effects of polyethylene glycol in colon carcinogenesis. Int J Oncol. 38:529–536. 2011.PubMed/NCBI

23 

Chien MH, Lee TS, Liang YC and Lee WS: β-Sitosterol inhibits cell cycle progression of rat aortic smooth muscle cells through increases of p21cip1 protein. J Agric Food Chem. 58:10064–10069. 2010.

24 

Fang Y, Yu S and Braley-Mullen H: TGF-β promotes proliferation of thyroid epithelial cells in IFN-γ(−/−) mice by down-regulation of p21 and p27 via AKT pathway. Am J Pathol. 180:650–660. 2011.

25 

Zuo S, Liu C, Wang J, et al: IGFBP-rP1 induces p21 expression through a p53-independent pathway, leading to cellular senescence of MCF-7 breast cancer cells. J Cancer Res Clin Oncol. 138:1045–1055. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Calabrese EJ: Hormesis: changing view of the dose-response, a personal account of the history and current status. Mutat Res. 511:181–189. 2002. View Article : Google Scholar : PubMed/NCBI

27 

McKean C, Tang L, Tang M, et al: Comparative acute and combinative toxicity of aflatoxin B1 and fumonisin B1 in animals and human cells. Food Chem Toxicol. 44:868–876. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Marin DE, Gouze ME, Taranu I and Oswald IP: Fumonisin B1 alters cell cycle progression and interleukin-2 synthesis in swine peripheral blood mononuclear cells. Mol Nutr Food Res. 51:1406–1412. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Fornelli F, Minervini F and Mulè G: Cytotoxicity induced by nivalenol, deoxynivalenol, and fumonisin B1 in the SF-9 insect cell line. In Vitro Cell Dev Biol Anim. 40:166–171. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Lewis CW, Smith JE, Anderson JG and Freshney RI: Increased cytotoxicity of food-borne mycotoxins toward human cell lines in vitro via enhanced cytochrome p450 expression using the MTT bioassay. Mycopathologia. 148:97–102. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Ramljak D, Calvert RJ, Wiesenfeld PW, et al: A potential mechanism for fumonisin B(1)-mediated hepatocarcinogenesis: cyclin D1 stabilization associated with activation of Akt and inhibition of GSK-3beta activity. Carcinogenesis. 21:1537–1546. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Wang W, Jones C, Ciacci-Zanella J, et al: Fumonisins and Alternaria alternata lycopersici toxins: sphinganine analog mycotoxins induce apoptosis in monkey kidney cells. Proc Natl Acad Sci USA. 93:3461–3465. 1996.

33 

Zhu X, Ohtsubo M, Böhmer RM, et al: Adhesion-dependent cell cycle progression linked to the expression of cyclin D1, activation of cyclin E-cdk2, and phosphorylation of the retinoblastoma protein. J Cell Biol. 133:391–403. 1996. View Article : Google Scholar : PubMed/NCBI

34 

Wang ZA and Kalderon D: Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells. Proc Natl Acad Sci USA. 106:21701–21706. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Fox PM, Vought VE, Hanazawa M, et al: Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline. Development. 138:2223–2234. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Zhang Y, Dickman MB and Jones C: The mycotoxin fumonisin B1 transcriptionally activates the p21 promoter through a cis-acting element containing two Sp1 binding sites. J Biol Chem. 274:12367–12371. 1999. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang S, Liu S, Yang L, Shi R and Sun G: Effect of fumonisin B1 on the cell cycle of normal human liver cells. Mol Med Rep 7: 1970-1976, 2013.
APA
Wang, S., Liu, S., Yang, L., Shi, R., & Sun, G. (2013). Effect of fumonisin B1 on the cell cycle of normal human liver cells. Molecular Medicine Reports, 7, 1970-1976. https://doi.org/10.3892/mmr.2013.1447
MLA
Wang, S., Liu, S., Yang, L., Shi, R., Sun, G."Effect of fumonisin B1 on the cell cycle of normal human liver cells". Molecular Medicine Reports 7.6 (2013): 1970-1976.
Chicago
Wang, S., Liu, S., Yang, L., Shi, R., Sun, G."Effect of fumonisin B1 on the cell cycle of normal human liver cells". Molecular Medicine Reports 7, no. 6 (2013): 1970-1976. https://doi.org/10.3892/mmr.2013.1447
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Liu S, Yang L, Shi R and Sun G: Effect of fumonisin B1 on the cell cycle of normal human liver cells. Mol Med Rep 7: 1970-1976, 2013.
APA
Wang, S., Liu, S., Yang, L., Shi, R., & Sun, G. (2013). Effect of fumonisin B1 on the cell cycle of normal human liver cells. Molecular Medicine Reports, 7, 1970-1976. https://doi.org/10.3892/mmr.2013.1447
MLA
Wang, S., Liu, S., Yang, L., Shi, R., Sun, G."Effect of fumonisin B1 on the cell cycle of normal human liver cells". Molecular Medicine Reports 7.6 (2013): 1970-1976.
Chicago
Wang, S., Liu, S., Yang, L., Shi, R., Sun, G."Effect of fumonisin B1 on the cell cycle of normal human liver cells". Molecular Medicine Reports 7, no. 6 (2013): 1970-1976. https://doi.org/10.3892/mmr.2013.1447
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team