Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July 2013 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July 2013 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry

  • Authors:
    • Xiaoqin Mao
    • Xiongfei Jia
    • Feng Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China, Department of Clinical Laboratory, Kunming General Hospital, Kunming, Yunnan 650032, P.R. China, Department of Pharmacy, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 128-132
    |
    Published online on: May 8, 2013
       https://doi.org/10.3892/mmr.2013.1464
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Propranolol (PRO), a nonselective β-adrenergic receptor (β-AR) antagonist, has two enantiomers, R(+)-PRO and S(-)-PRO, which have diverse biological effects. For example, S(-)-PRO blocks the β-receptor ~100 times more strongly than R(+)-PRO. However, the signaling pathway that causes this difference remains unclear. This pathway may affect the expression of numerous proteins, some of which play key roles during the drug action process. Therefore, we treated human umbilical vein endothelial cells (HUVECs) with R(+)-PRO and S(-)-PRO in order to identify differentially expressed proteins and to determine their functions in the drug action process. Of the 22 differentially expressed protein spots investigated, 14 demonstrated higher expression levels in the R(+)-PRO-treated cells, while 8 demonstrated lower expression levels in the same cells. Mass spectrometry identified 10 of the differentially expressed proteins: 4 signaling molecules, 2 metabolic enzymes, 3 heat shock proteins and 1 cytoskeleton protein. Our results suggest that these differentially expressed proteins, particularly guanine nucleotide-binding protein subunit β-2-like 1 (GBLP), are the key biomacromolecules underlying the mechanism by which PRO enantiomers induce stereoselective cellular responses. The results aid in clarifying the role of PRO in the treatment of arrhythmia and angina.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Steiner S and Anderson NL: Pharmaceutical proteomics. Ann NY Acad Sci. 919:48–51. 2000. View Article : Google Scholar

2 

Graves PR, Kwiek JJ, Fadden P, et al: Discovery of novel targets of quinoline drugs in the human purine binding proteome. Mol Pharmacol. 62:1364–1372. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Bruneau JM, Maillet J, Tagat E, et al: Drug induced protome changes in Candida albicans: comparison of the effect of beta(1,3) glucan synthase inhibitors and two triazoles, fluconazole and itraconazole. Proteomics. 3:325–336. 2003.PubMed/NCBI

4 

You QD and Lin GQ: Chiral Drugs: Research and Application. 34. Chemical Industry Press; Beijing: pp. 230–234. 2003

5 

Lee DH, Jacobs DR Jr, Gross M, et al: Gamma-glutamyltransferase is a predictor of incident diabetes and hypertension: the Coronary Artery Risk Development in Young Adults (CARDIA) Study. Clin Chem. 49:1358–1366. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Lee DH, Ha MH, Kim JH, et al: Gamma-glutamyltransferase and diabetes - a 4 years follow-up study. Diabetologia. 46:359–364. 2003.PubMed/NCBI

7 

Dorsch M, Danial NN, Rothman PB and Goff SP: A thrombopoietin receptor mutant deficient in Jak-STAT activation mediates proliferation but not differentiation in UT-7 cells. Blood. 94:2676–2685. 1999.PubMed/NCBI

8 

Guil S, de La Iglesia N, Fernández-Larrea J, et al: Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus. Cancer Res. 63:5178–5187. 2003.PubMed/NCBI

9 

Kim IS, Ryang YS, Kim YS, et al: Leukotactin-1-induced ERK activation is mediated via Gi/Go protein/PLC/PKC delta/Ras cascades in HOS cells. Life Sci. 73:447–459. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Mochly-Rosen D, Khaner H and Lopez J: Identification of intracellular receptor proteins for activated protein kinase C. Proc Natl Acad Sci USA. 88:3997–4000. 1991. View Article : Google Scholar : PubMed/NCBI

11 

Mamidipudi V, Dhillon NK, Parman T, Miller LD, Lee KC and Cartwright CA: RACK1 inhibits colonic cell growth by regulating Src activity at cell cycle checkpoints. Oncogene. 26:2914–2924. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Bolger GB, Baillie GS, Li X, et al: Scanning peptide array analyses identify overlapping binding sites for the signaling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5. Biochem J. 398:23–36. 2006. View Article : Google Scholar

13 

Kiely PA, O'Gorman D, Luong K, Ron D and O'Connor R: Insulin-like growth factor I controls a mutually exclusive association of RACK1 with protein phosphatase 2A and beta1 integrin to promote cell migration. Mol Cell Biol. 26:4041–4051. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Hermanto V, Zong CS, Li W and Wang LH: RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix. Mol Cell Biol. 22:2345–2365. 2002. View Article : Google Scholar

15 

Yaka R, Thornton C, Vagts AJ, Phamluong K, Bonci A and Ron D: NMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1. Proc Natl Acad Sci USA. 99:5710–5715. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Battaini F, Pascale A, Paoletti R and Govoni S: The role of anchoring protein RACK1 in PKC activation in the ageing rat brain. Trends Neurosci. 20:410–415. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Qiu Y, Mao T, Zhang Y, et al: A crucial role for RACK1 in the regulation of glucose-stimulated IRE1alpha activation in pancreatic beta cells. Sci Signal. 3:ra72010.PubMed/NCBI

18 

Patterson RL, van Rossum DB, Barrow RK and Snyder SH: RACK1 binds to inositol 1,4,5-trisphosphate receptors and mediates Ca2+ release. Proc Natl Acad Sci USA. 101:2328–2332. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Mao X, Jia X and Qiu F: Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry. Mol Med Rep 8: 128-132, 2013.
APA
Mao, X., Jia, X., & Qiu, F. (2013). Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry. Molecular Medicine Reports, 8, 128-132. https://doi.org/10.3892/mmr.2013.1464
MLA
Mao, X., Jia, X., Qiu, F."Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry". Molecular Medicine Reports 8.1 (2013): 128-132.
Chicago
Mao, X., Jia, X., Qiu, F."Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry". Molecular Medicine Reports 8, no. 1 (2013): 128-132. https://doi.org/10.3892/mmr.2013.1464
Copy and paste a formatted citation
x
Spandidos Publications style
Mao X, Jia X and Qiu F: Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry. Mol Med Rep 8: 128-132, 2013.
APA
Mao, X., Jia, X., & Qiu, F. (2013). Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry. Molecular Medicine Reports, 8, 128-132. https://doi.org/10.3892/mmr.2013.1464
MLA
Mao, X., Jia, X., Qiu, F."Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry". Molecular Medicine Reports 8.1 (2013): 128-132.
Chicago
Mao, X., Jia, X., Qiu, F."Enantioselective pharmacodynamics of propranolol in HUVEC cells: A study using chiral 2D gel electrophoresis and mass spectrometry". Molecular Medicine Reports 8, no. 1 (2013): 128-132. https://doi.org/10.3892/mmr.2013.1464
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team