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Article

Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach

  • Authors:
    • Jin-Hee Hwang
    • Jin-Ju Oh
    • Tao Wang
    • Yong-Cheng Jin
    • Jae-Sung Lee
    • Jong-Ryeol Choi
    • Kyu-Sup Lee
    • Jong-Kil Joo
    • Hong-Gu Lee
  • View Affiliations / Copyright

    Affiliations: Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul 143‑701, Republic of Korea, Natural Product Clinical Research Center, Clinical Research Center, Pusan National University School of Medicine, Busan 602-739, Republic of Korea, Department of Animal Science, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P.R. China, Department of Obstetrics and Gynecology, School of Medicine, Pusan National University, Yangsan, Gyeongnam 626-770, Republic of Korea
  • Pages: 183-194
    |
    Published online on: May 10, 2013
       https://doi.org/10.3892/mmr.2013.1469
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Abstract

Endometriosis is a gynecological disease defined as the presence of endometrial tissue outside the uterine cavity, which is caused by various factors. Proteomic analysis of two sets of eutopic endometrial cells collected from the menstrual blood of females with (n=6; n=3) or without (n=6; n=3) endometriosis was performed to identify novel potential biomarkers for endometriosis. The data revealed that samples from endometriosis patients had stem cell characteristics, as they had higher mRNA expression levels of octamer-binding transcription factor 4 (Oct-4), C-X-C chemokine receptor type 4 (CXCR4), SRY-box containing gene 2 (SOX2) and mesenchymal-epithelial transition factor (MET) compared with that of the normal controls. Three proteins, collapsin response mediator protein 2 (CRMP2), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) and myosin regulatory light polypeptide 9 (MYL9), were simultaneously identified from the two sets of samples from females with or without endometriosis by two-dimensional electrophoresis (2-DE). A difference in CRMP2 expression was confirmed with western blotting. Taken together, the results suggest that CRMP2 plays a role in the pathogenesis of endometriosis.
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Copy and paste a formatted citation
Spandidos Publications style
Hwang J, Oh J, Wang T, Jin Y, Lee J, Choi J, Lee K, Joo J and Lee H: Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Mol Med Rep 8: 183-194, 2013.
APA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J. ... Lee, H. (2013). Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Molecular Medicine Reports, 8, 183-194. https://doi.org/10.3892/mmr.2013.1469
MLA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8.1 (2013): 183-194.
Chicago
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8, no. 1 (2013): 183-194. https://doi.org/10.3892/mmr.2013.1469
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang J, Oh J, Wang T, Jin Y, Lee J, Choi J, Lee K, Joo J and Lee H: Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Mol Med Rep 8: 183-194, 2013.
APA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J. ... Lee, H. (2013). Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach. Molecular Medicine Reports, 8, 183-194. https://doi.org/10.3892/mmr.2013.1469
MLA
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8.1 (2013): 183-194.
Chicago
Hwang, J., Oh, J., Wang, T., Jin, Y., Lee, J., Choi, J., Lee, K., Joo, J., Lee, H."Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach". Molecular Medicine Reports 8, no. 1 (2013): 183-194. https://doi.org/10.3892/mmr.2013.1469
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