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Article

Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma

  • Authors:
    • Shaoshan Han
    • Lei Han
    • Hao Sun
    • Xianfeng Zan
    • Zhenyu Zhou
    • Kedong Xu
    • Yingmin Yao
    • Qingguang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Immunology and Pathogenic Biology, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 81-88
    |
    Published online on: May 10, 2013
       https://doi.org/10.3892/mmr.2013.1471
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Abstract

Whether a focal adhesion kinase (FAK)‑Krüppel‑like factor 8 (KLF8)‑matrix metalloproteinase (MMP)‑9/E‑cadherin signaling axis exists in hepatocellular carcinoma (HCC) remains unknown. In the present study, KLF8 expression and its clinicopathological significance in HCC was investigated to determine the correlation between KLF8 and FAK, MMP‑9 and E‑cadherin expression. Tissues were obtained from 60 surgically resected HCC and normal tumor‑adjacent tissues. KLF8, FAK, MMP‑9 and E‑cadherin expression levels were examined by quantitative real‑time reverse transcription polymerase chain reaction (qRT‑PCR) and immunohistochemistry. In addition, KLF8, FAK, MMP‑9 and E‑cadherin protein expression levels were examined by western blot analysis in 5 portal vein cancer emboli and corresponding HCC tissues. The clinicopathological data of the HCC patients were retrospectively analyzed. KLF8 mRNA expression was found to be significantly upregulated in HCC tumor tissues compared with normal tumor‑adjacent tissues (P<0.05). KLF8 protein was highly expressed in portal vein cancer emboli. KLF8 expression level was significantly higher in tumors with advanced TNM stages and vascular invasion compared with that in tumors with early TNM stage and absence of vascular invasion (P<0.05). KLF8 protein and mRNA expression in HCC positively correlated with that of FAK (r=0.362, P<0.001; and r=0.377, P<0.01, respectively) and MMP‑9 (r=0.392, P<0.01; and r=0.336, P<0.01, respectively), but negatively correlated with E‑cadherin (r=‑0.364, P<0.01; and r=‑0.410, P<0.01, respectively). Results of the current study highlight a novel FAK‑KLF8‑MMP‑9/E‑cadherin signaling axis during HCC progression.
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1 

Farazi PA and DePinho RA: Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer. 6:674–687. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Eguchi S, Kanematsu T, Arii S, et al: Recurrence-free survival more than 10 years after liver resection for hepatocellular carcinoma. Br J Surg. 98:552–557. 2011.PubMed/NCBI

3 

El-Serag HB, Marrero JA, Rudolph L and Reddy KR: Diagnosis and treatment of hepatocellular carcinoma. Gastroenterology. 134:1752–1763. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Tang ZY, Ye SL, Liu YK, et al: A decade’s studies on metastasis of hepatocellular carcinoma. J Cancer Res Clin Oncol. 130:187–196. 2004.

5 

McLean GW, Carragher NO, Avizienyte E, Evans J, Brunton VG and Frame MC: The role of focal-adhesion kinase in cancer. A new therapeutic opportunity. Nat Rev Cancer. 5:505–515. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Watermann DO, Gabriel B, Jager M, et al: Specific induction of pp125 focal adhesion kinase in human breast cancer. Br J Cancer. 93:694–698. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Miyazaki T, Kato H, Nakajima M, et al: FAK overexpression is correlated with tumour invasiveness and lymph node metastasis in oesophageal squamous cell carcinoma. Br J Cancer. 89:140–145. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Rovin JD, Frierson HF Jr, Ledinh W, Parsons JT and Adams RB: Expression of focal adhesion kinase in normal and pathologic human prostate tissues. Prostate. 53:124–132. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Aronsohn MS, Brown HM, Hauptman G and Kornberg LJ: Expression of focal adhesion kinase and phosphorylated focal adhesion kinase in squamous cell carcinoma of the larynx. Laryngoscope. 113:1944–1948. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Itoh S, Maeda T, Shimada M, et al: Role of expression of focal adhesion kinase in progression of hepatocellular carcinoma. Clin Cancer Res. 10:2812–2817. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Wang XH, Zheng MZ, Liu G, et al: Krüppel-like factor 8 induces epithelial to mesenchymal transition and epithelial cell invasion. Cancer Res. 67:7184–7193. 2007.

12 

Fu WJ, Li JC, Wu XY, et al: Small interference RNA targeting Krüppel-like factor 8 inhibits the renal carcinoma 786–0 cells growth in vitro and in vivo. J Cancer Res Clin Oncol. 136:1255–1265. 2010.

13 

Wei H, Wang X, Gan B, et al: Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation. J Biol Chem. 281:16664–16671. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Liu L, Liu N, Xu M, et al: Lentivirus-delivered Krüppel-like factor 8 small interfering RNA inhibits gastric cancer cell growth in vitro and in vivo. Tumour Biol. 33:53–61. 2012.

15 

Li JC, Yang XR, Sun HX, et al: Up-regulation of Krüppel-like factor 8 promotes tumor invasion and indicates poor prognosis for hepatocellular carcinoma. Gastroenterology. 139:2146–2157. 2010.

16 

Zhao J, Bian ZC, Yee K, Chen BP, Chien S and Guan JL: Identification of transcription factor KLF8 as a downstream target of focal adhesion kinase in its regulation of cyclin D1 and cell cycle progression. Mol Cell. 11:1503–1515. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Wang X, Urvalek AM, Liu J and Zhao J: Activation of KLF8 transcription by focal adhesion kinase in human ovarian epithelial and cancer cells. J Biol Chem. 283:13934–13942. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wang X, Lu H, Urvalek AM, et al: KLF8 promotes human breast cancer cell invasion and metastasis by transcriptional activation of MMP-9. Oncogene. 30:1901–1911. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Tu K, Zheng X, Zan X, Han S, Yao Y and Liu Q: Evaluation of Fbxw7 expression and its correlation with the expression of c-Myc, cyclin E and p53 in human hepatocellular carcinoma. Hepatol Res. 42:904–910. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Pantel K and Brakenhoff RH: Dissecting the metastatic cascade. Nat Rev Cancer. 4:448–456. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Fujii T, Koshikawa K, Nomoto S, et al: Focal adhesion kinase is overexpressed in hepatocellular carcinoma and can be served as an independent prognostic factor. J Hepatol. 41:104–111. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Peck-Radosavljevic M: Back to basics: Staging and prognosis in HCC for medical oncologist. J Hepatol. 56:488–489. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Chen JS, Wang Q, Fu XH, et al: Involvement of PI3K/PTEN/AKT/mTOR pathway in invasion and metastasis in hepatocellular carcinoma: association with MMP-9. Hepatol Res. 39:177–186. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Chen R, Cui J, Xu C, et al: The significance of MMP-9 over MMP-2 in HCC invasiveness and recurrence of hepatocellular carcinoma after curative resection. Ann Surg Oncol. 19(Suppl 3): S375–S384. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

27 

van Zijl F, Zulehner G, Petz M, et al: Epithelial-mesenchymal transition in hepatocellular carcinoma. Future Oncol. 5:1169–1179. 2009.

28 

Lee TK, Poon RT, Yuen AP, et al: Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition. Clin Cancer Res. 12:5369–5376. 2006. View Article : Google Scholar

29 

Lee S, Lee HJ, Kim JH, Lee HS, Jang JJ and Kang GH: Aberrant CpG island hypermethylation along multistep hepatocarcinogenesis. Am J Pathol. 163:1371–1378. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Liu J, Lian Z, Han S, et al: Downregulation of E-cadherin by hepatitis B virus X antigen in hepatocellullar carcinoma. Oncogene. 25:1008–1017. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Yang MH, Chen CL, Chau GY, et al: Comprehensive analysis of the independent effect of twist and snail in promoting metastasis of hepatocellular carcinoma. Hepatology. 50:1464–1474. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Zuo JH, Zhu W, Li MY, et al: Activation of EGFR promotes squamous carcinoma SCC10A cell migration and invasion via inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin. J Cell Biochem. 112:2508–2517. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Han S, Han L, Sun H, Zan X, Zhou Z, Xu K, Yao Y and Liu Q: Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma. Mol Med Rep 8: 81-88, 2013.
APA
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K. ... Liu, Q. (2013). Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma. Molecular Medicine Reports, 8, 81-88. https://doi.org/10.3892/mmr.2013.1471
MLA
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K., Yao, Y., Liu, Q."Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma". Molecular Medicine Reports 8.1 (2013): 81-88.
Chicago
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K., Yao, Y., Liu, Q."Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma". Molecular Medicine Reports 8, no. 1 (2013): 81-88. https://doi.org/10.3892/mmr.2013.1471
Copy and paste a formatted citation
x
Spandidos Publications style
Han S, Han L, Sun H, Zan X, Zhou Z, Xu K, Yao Y and Liu Q: Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma. Mol Med Rep 8: 81-88, 2013.
APA
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K. ... Liu, Q. (2013). Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma. Molecular Medicine Reports, 8, 81-88. https://doi.org/10.3892/mmr.2013.1471
MLA
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K., Yao, Y., Liu, Q."Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma". Molecular Medicine Reports 8.1 (2013): 81-88.
Chicago
Han, S., Han, L., Sun, H., Zan, X., Zhou, Z., Xu, K., Yao, Y., Liu, Q."Krüppel‑like factor expression and correlation with FAK, MMP‑9 and E‑cadherin expression in hepatocellular carcinoma". Molecular Medicine Reports 8, no. 1 (2013): 81-88. https://doi.org/10.3892/mmr.2013.1471
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