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Article

A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage

  • Authors:
    • Zhaoliang Su
    • Siamak Sandoghchian Shotorbani
    • Xugan Jiang
    • Rui Ma
    • Huiling Shen
    • Fanzhi Kong
    • Huaxi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Immunology and Laboratory Immunology, Jiangsu University, Zhenjiang 212013, P.R. China, The Affiliated People's Hospital of Jiangsu University, Zhenjiang 212008, P.R. China
  • Pages: 113-117
    |
    Published online on: May 14, 2013
       https://doi.org/10.3892/mmr.2013.1476
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Abstract

At present, collagen‑induced arthritis (CIA) is the best known and most extensively used model for the immunological and pathological characteristics of human rheumatoid arthritis (RA). This model is useful not only in aiding our understanding of the pathogenesis of this disease, but also in the development of new therapies. Bovine, porcine and human collagen has been used to induce CIA; however, response has been identified to vary between strains and injection conditions, and false positive results and reduced potency are common as a result of minor contaminants or deglycosylated protein. Therefore, in the present study, type II collagen (CII) was isolated and purified from chicken sternal cartilage and was found to successfully induce the RA model. Furthermore, T helper 17 (Th17) cells were observed to infiltrate the joint on day 45 following induction by CII. In vitro, expression of toll‑like receptor 2 (TLR2) increased in peritoneal macrophages stimulated by CII. In addition, blockage of TLR2 was identified to markedly decrease levels of TGF‑β and IL‑6 in the cell culture supernatant. The results indicate that CII isolated from chicken sternal cartilage may be recognized by TLR2 on macrophages, leading to TGF‑β and IL‑6 production and subsequent activation of Th17 cells which mediates CIA development.
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Copy and paste a formatted citation
Spandidos Publications style
Su Z, Shotorbani SS, Jiang X, Ma R, Shen H, Kong F and Xu H: A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage. Mol Med Rep 8: 113-117, 2013.
APA
Su, Z., Shotorbani, S.S., Jiang, X., Ma, R., Shen, H., Kong, F., & Xu, H. (2013). A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage. Molecular Medicine Reports, 8, 113-117. https://doi.org/10.3892/mmr.2013.1476
MLA
Su, Z., Shotorbani, S. S., Jiang, X., Ma, R., Shen, H., Kong, F., Xu, H."A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage". Molecular Medicine Reports 8.1 (2013): 113-117.
Chicago
Su, Z., Shotorbani, S. S., Jiang, X., Ma, R., Shen, H., Kong, F., Xu, H."A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage". Molecular Medicine Reports 8, no. 1 (2013): 113-117. https://doi.org/10.3892/mmr.2013.1476
Copy and paste a formatted citation
x
Spandidos Publications style
Su Z, Shotorbani SS, Jiang X, Ma R, Shen H, Kong F and Xu H: A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage. Mol Med Rep 8: 113-117, 2013.
APA
Su, Z., Shotorbani, S.S., Jiang, X., Ma, R., Shen, H., Kong, F., & Xu, H. (2013). A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage. Molecular Medicine Reports, 8, 113-117. https://doi.org/10.3892/mmr.2013.1476
MLA
Su, Z., Shotorbani, S. S., Jiang, X., Ma, R., Shen, H., Kong, F., Xu, H."A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage". Molecular Medicine Reports 8.1 (2013): 113-117.
Chicago
Su, Z., Shotorbani, S. S., Jiang, X., Ma, R., Shen, H., Kong, F., Xu, H."A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage". Molecular Medicine Reports 8, no. 1 (2013): 113-117. https://doi.org/10.3892/mmr.2013.1476
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