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Article

Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma

  • Authors:
    • Miao He
    • Jing Fan
    • Rong Jiang
    • Wei‑Xue Tang
    • Zi‑Wei Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Emergency, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Laboratory of Stem Cell and Tissue Engineering, Institute of Basic Medicine of Chongqing Medical University, Chongqing 400016, P.R. China, Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 942-948
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    Published online on: July 2, 2013
       https://doi.org/10.3892/mmr.2013.1566
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Abstract

The aim of the present study was to investigate the protein expression of DNA methyltransferases (DNMTs) and genomic DNA methylation status of genomes in gastric signet ring cell carcinoma (SRC). Immunohistochemistry was performed to analyze DNMT expression and methylated DNA immunoprecipitation microarray (MeDIP‑chip) and MeDIP quantitative real‑time PCR (MeDIP‑qPCR) were performed to analyze the genomic DNA methylation status in gastric SRC tissue. An increase in DNMT1 and decrease in DNMT3A expression in SRC tissue was observed compared with matched non‑cancerous tissue. However, expression of other DNMTs, DNMT2, DNMT3B and DNMT3L, was not found to differ significantly between carcinoma and control. The MeDIP‑chip assay revealed that methylation of gene promoters and CpG islands in SRC was higher than those in matched control tissue. However, MeDIP‑qPCR analysis demonstrated that specific tumor‑related genes, including ABL2, FGF18, TRAF2, EGFL7 and RAB33A were aberrantly hypomethylated in SRC tissue. Results of the current study indicate that gastric SRC may produce complex patterns of aberrant DNA methylation and DNMT expression.
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Copy and paste a formatted citation
Spandidos Publications style
He M, Fan J, Jiang R, Tang WX and Wang ZW: Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma. Mol Med Rep 8: 942-948, 2013.
APA
He, M., Fan, J., Jiang, R., Tang, W., & Wang, Z. (2013). Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma. Molecular Medicine Reports, 8, 942-948. https://doi.org/10.3892/mmr.2013.1566
MLA
He, M., Fan, J., Jiang, R., Tang, W., Wang, Z."Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma". Molecular Medicine Reports 8.3 (2013): 942-948.
Chicago
He, M., Fan, J., Jiang, R., Tang, W., Wang, Z."Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma". Molecular Medicine Reports 8, no. 3 (2013): 942-948. https://doi.org/10.3892/mmr.2013.1566
Copy and paste a formatted citation
x
Spandidos Publications style
He M, Fan J, Jiang R, Tang WX and Wang ZW: Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma. Mol Med Rep 8: 942-948, 2013.
APA
He, M., Fan, J., Jiang, R., Tang, W., & Wang, Z. (2013). Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma. Molecular Medicine Reports, 8, 942-948. https://doi.org/10.3892/mmr.2013.1566
MLA
He, M., Fan, J., Jiang, R., Tang, W., Wang, Z."Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma". Molecular Medicine Reports 8.3 (2013): 942-948.
Chicago
He, M., Fan, J., Jiang, R., Tang, W., Wang, Z."Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma". Molecular Medicine Reports 8, no. 3 (2013): 942-948. https://doi.org/10.3892/mmr.2013.1566
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