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Article

Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway

  • Authors:
    • Jia-He Wang
    • Liang Kan
    • Lin-Hua Shu
    • Nan Wang
    • Nai-Jing Li
    • Meng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 787-793
    |
    Published online on: July 4, 2013
       https://doi.org/10.3892/mmr.2013.1571
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Abstract

Numerous studies have demonstrated that pseudolaric acid B (PAB) promotes apoptosis in several cancer cell lines. However, thus far, the effect of PAB on human leukemia cells has not been evaluated. In the present study, the antitumor activity and molecular mechanisms of PAB in human leukemia U937 cells were investigated. It was demonstrated that PAB induced U937 cell apoptosis, which was confirmed by typical morphological changes and Annexin V‑fluorescein isothiocyanate staining. PAB was observed to activate a caspase‑dependent apoptotic pathway in U937 cells through the regulation of the Bcl‑2 family protein-mediated mitochondrial pathway. Furthermore, the activities of caspase‑3 and -9 were increased following treatment with PAB. In conclusion, to the best of our knowledge, this study demonstrated for the first time that PAB was able to enhance the apoptosis of U937 cells, at least in part, through the activation of the mitochondrial death pathway. Moreover, the activation of caspase‑3 and -9 mediated the apoptotic induction.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Kan L, Shu L, Wang N, Li N and Zhang M: Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway. Mol Med Rep 8: 787-793, 2013.
APA
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., & Zhang, M. (2013). Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway. Molecular Medicine Reports, 8, 787-793. https://doi.org/10.3892/mmr.2013.1571
MLA
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., Zhang, M."Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway". Molecular Medicine Reports 8.3 (2013): 787-793.
Chicago
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., Zhang, M."Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway". Molecular Medicine Reports 8, no. 3 (2013): 787-793. https://doi.org/10.3892/mmr.2013.1571
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Kan L, Shu L, Wang N, Li N and Zhang M: Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway. Mol Med Rep 8: 787-793, 2013.
APA
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., & Zhang, M. (2013). Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway. Molecular Medicine Reports, 8, 787-793. https://doi.org/10.3892/mmr.2013.1571
MLA
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., Zhang, M."Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway". Molecular Medicine Reports 8.3 (2013): 787-793.
Chicago
Wang, J., Kan, L., Shu, L., Wang, N., Li, N., Zhang, M."Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9‑mediated activation of the mitochondrial death pathway". Molecular Medicine Reports 8, no. 3 (2013): 787-793. https://doi.org/10.3892/mmr.2013.1571
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