Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September 2013 Volume 8 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 2013 Volume 8 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction

  • Authors:
    • Katarzyna Szadujkis‑Szadurska
    • Grzegorz Grzesk
    • Leszek  Szadujkis‑Szadurski
    • Marta Gajdus
    • Bartosz Malinowski
    • Michal Wicinski
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Therapeutics, Collegium Medicum Nicolaus Copernicus University, Bydgoszcz 85-094, Poland
  • Pages: 914-918
    |
    Published online on: July 8, 2013
       https://doi.org/10.3892/mmr.2013.1574
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to establish the involvement of acetylcholine (Ach) and calcium ions in modulating contractions induced by Bay K8644 (an agonist of calcium channels located in the cell membrane) and KCl (at depolarizing concentrations), and also to examine the importance of the vascular endothelium in the activity of Bay K8644. The study was performed on perfused Wistar rat tail arteries. Contraction induced by Bay K8644 with the participation of intracellular (in calcium‑free physiological salt solution, FPSS) and extracellular (in physiological salt solution, PSS, following the emptying of the cellular Ca2+ stores) pools of Ca2+ and the addition of nitro-L-arginine (L‑NNA; nitric oxide synthase inhibitor) or 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one (ODQ; an inhibitor of soluble guanylyl cyclase) was studied. In addition, the effect of Ach on the contraction response was analyzed and the results were compared with the depolarizing action of KCl. The effects of 8Br‑cGMP on the artery contraction induced by Bay K8644 prior to and following removal of the endothelium were compared. Bay K8644 and KCl in PSS induced vascular contraction, which was reduced with the addition of Ach. The spasmolytic Ach action did not occur in the presence of L‑NNA and ODQ. 8Br‑cGMP reduced the contraction of arterial walls (with and without endothelium) induced by Bay K8644. The increase in vascular tone induced by Bay K8644 and KCl was independent of the intracellular calcium ion pool. The relaxant effect of Ach on the responses stimulated by Bay K8644 and KCl indicated the participation of nitric oxide in modulating the reactivity of the arteries to the factors examined, resulting in an influx of Ca2+ into the cell.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Furchgott RF and Zawadzki JW: The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 288:373–376. 1980. View Article : Google Scholar : PubMed/NCBI

2 

Lüscher TF and Barton M: Biology of the endothelium. Clin Cardiol. 20(Suppl 2): II-3–II-10. 1997.

3 

Palmer RM, Ashton DS and Moncada S: Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 333:664–666. 1988. View Article : Google Scholar : PubMed/NCBI

4 

Furchgott RF and Vanhoutte PM: Endothelium-derived relaxing and contracting factors. FASEB J. 3:2007–2018. 1989.PubMed/NCBI

5 

Murad F: The nitric oxide-cyclic GMP signal transduction system for intracellular and intercellular communication. Recent Prog Horm Res. 49:239–248. 1994.PubMed/NCBI

6 

Blatter LA and Wier WG: Nitric oxide decreases [Ca2+]i in vascular smooth muscle by inhibition of the calcium current. Cell Calcium. 15:122–131. 1994.

7 

Collins P, Griffith TM, Henderson AH and Lewis MJ: Endothelium-derived relaxing factor alters calcium fluxes in rabbit aorta: a cyclic guanosine monophosphate-mediated effect. J Physiol. 381:427–437. 1986. View Article : Google Scholar

8 

Karaki H, Sato K, Ozaki H and Murakami K: Effects of sodium nitroprusside on cytosolic calcium level in vascular smooth muscle. Eur J Pharmacol. 156:259–266. 1988. View Article : Google Scholar : PubMed/NCBI

9 

McDaniel NL, Chen XL, Singer HA, Murphy RA and Rembold CM: Nitrovasodilators relax arterial smooth muscle by decreasing [Ca2+]i and uncoupling stress from myosin phosphorylation. Am J Physiol. 263:C461–C467. 1992.

10 

Murad F: Cyclic guanosine monophosphate as a mediator of vasodilation. J Clin Invest. 78:1–5. 1986. View Article : Google Scholar : PubMed/NCBI

11 

Moncada S, Palmer RM and Higgs EA: Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 43:109–142. 1991.

12 

Van Rossum JM: Cumulative dose-response curves. II Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. Arch Int Pharmacodyn Ther. 143:299–330. 1963.PubMed/NCBI

13 

Koller A, Sun D, Huang A and Kaley G: Corelease of nitric oxide and prostaglandins mediates flow-dependent dilation of rat gracilis muscle arterioles. Am J Physiol. 267:H326–H332. 1994.PubMed/NCBI

14 

Szadujkis-Szadurska K, Slupski M, Szadujkis-Szadurski R, Szadujkis-Szadurski L, Jasiñski M and Kolodziejska R: The role of the endothelium in the regulation of vascular smooth muscle cell contractions induced by angiotensin II after ischemia and reperfusion. Arch Pharm Res. 33:1019–1024. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Dignan RJ, Dyke CM, Abd-Elfattah AS, Lutz HA, Yeh T Jr, Lee KF, Parmar J and Wechsler AS: Coronary artery endothelial cell and smooth muscle dysfunction after global myocardial ischemia. Ann Thorac Surg. 53:311–317. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Fullerton DA, Hahn AR, Koike K, Banerjee A and Harken AH: Intracellular mechanisms of pulmonary vasomotor dysfunction in acute lung injury caused by mesenteric ischemia-reperfusion. Surgery. 114:360–367. 1993.PubMed/NCBI

17 

Fullerton DA, Mitchell MB, McIntyre RC Jr, Banerjee A, Campbell DN, Harken AH and Grover FL: Cold ischemia and reperfusion each produce pulmonary vasomotor dysfunction in the transplanted lung. J Thorac Cardiovasc Surg. 106:1213–1217. 1993.PubMed/NCBI

18 

Hashimoto K, Pearson PJ, Schaff HV and Cartier R: Endothelial cell dysfunction after ischemic arrest and reperfusion: a possible mechanism of myocardial injury during reflow. J Thorac Cardiovasc Surg. 102:688–694. 1991.PubMed/NCBI

19 

Dauber IM, VanBenthuysen KM, McMurtry IF, Wheeler GS, Lesnefsky EJ, Horwitz LD and Weil JV: Functional coronary microvascular injury evident as increased permeability due to brief ischemia and reperfusion. Circ Res. 66:986–998. 1990. View Article : Google Scholar : PubMed/NCBI

20 

Pesic A, Madden JA, Pesic M and Rusch NJ: High blood pressure upregulates arterial L-type Ca2+ channels: is membrane depolarization the signal? Circ Res. 94:e97–e104. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Pratt PF, Bonnet S, Ludwig LM, Bonnet P and Rusch NJ: Upregulation of L-type Ca2+ channels in mesenteric and skeletal arteries of SHR. Hypertension. 40:214–219. 2002.

22 

Szadujkis-Szadurski R, Tafil-Klawe M, Szadujkis-Szadurska K, Szadujkis-Szadurski L, Slupski M, Grzesk G, Matusiak G, Gajdus M and Glaza I: Modulation of the contractile effect of Bay K8644 on human vascular smooth muscle cells by acetylocholine and calcium ions. Med Biol Sci. 24:59–64. 2010.

23 

Szadujkis-Szadurska K, Grzesk G, Szadujkis-Szadurski L, Gajdus M and Matusiak G: Role of nitric oxide and cGMP in modulation of vascular contraction induced by angiotensin II and Bay K8644 during ischemia/reperfusion. Exp Ther Med. 5:616–620. 2013.PubMed/NCBI

24 

Iesaki T and Wolin MS: Thiol oxidation activates a novel redox-regulated coronary vasodilator mechanism involving inhibition of Ca2+ influx. Arterioscler Thromb Vasc Biol. 20:2359–2365. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Matchkov VV, Aalkjaer C and Nilsson H: A cyclic GMP- dependent calcium-activated chloride current in smooth-muscle cells from rat mesenteric resistance arteries. J Gen Physiol. 123:121–134. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Piper AS and Large WA: Direct effect of Ca2+-calmodulin on cGMP-activated Ca2+-dependent Cl-channels in rat mesenteric artery myocytes. J Physiol. 559:449–457. 2004.PubMed/NCBI

27 

Szadujkis-Szadurski L, Talar J, Wisniewski K, Tomaszewski W, Lukowicz M and Szadujkis-Szadurski R: Modulatory effects of laser radiation on extracellular and intracellular calcium pool and vascular resistance of rat’s tail artery. Physiother Pol. 2:11–20. 2002.

28 

Ji J, Benishin CG and Pang PK: Nitric oxide selectively inhibits intracellular Ca2+ release elicited by inositol trisphosphate but not caffeine in rat vascular smooth muscle. J Pharmacol Exp Ther. 285:16–21. 1998.PubMed/NCBI

29 

Brozovich FV: Rho signaling: agonist stimulation and depolarization come together. Circ Res. 93:481–483. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Liu C, Zuo J, Pertens E, Helli PB and Janssen LJ: Regulation of Rho/ROCK signaling in airway smooth muscle by membrane potential and [Ca2+]i. Am J Physiol Lung Cell Mol Physiol. 289:L574–L582. 2005.PubMed/NCBI

31 

Somlyo AV and Somlyo AP: Electromechanical and pharmacomechanical coupling in vascular smooth muscle. J Pharmacol Exp Ther. 159:129–145. 1968.PubMed/NCBI

32 

Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ and Sato K: Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev. 49:157–230. 1997.PubMed/NCBI

33 

Kobayashi S, Kanaide H and Nakamura M: Complete overlap of caffeine- and K+ depolarization-sensitive intracellular calcium storage site in cultured rat arterial smooth muscle cells. J Biol Chem. 261:15709–15713. 1986.PubMed/NCBI

34 

Ureña J, del Valle-Rodríguez A and López-Barneo J: Metabotropic Ca2+ channel-induced calcium release in vascular smooth muscle. Cell Calcium. 42:513–520. 2007.

35 

Yanagisawa T and Okada Y: KCl depolarization increases Ca2+ sensitivity of contractile elements in coronary arterial smooth muscle. Am J Physiol. 267:H614–H621. 1994.PubMed/NCBI

36 

Ratz PH, Berg KM, Urban NH and Miner AS: Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus. Am J Physiol Cell Physiol. 288:C769–C783. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Ratz PH and Miner AS: Role of protein kinase Czeta and calcium entry in KCl-induced vascular smooth muscle calcium sensitization and feedback control of cellular calcium levels. J Pharmacol Exp Ther. 328:399–408. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Sakurada S, Takuwa N, Sugimoto N, Wang Y, Seto M, Sasaki Y and Takuwa Y: Ca2+-dependent activation of Rho and Rho kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction. Circ Res. 93:548–556. 2003.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Szadujkis‑Szadurska K, Grzesk G, Szadujkis‑Szadurski L, Gajdus M, Malinowski B and Wicinski M: Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction. Mol Med Rep 8: 914-918, 2013.
APA
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., & Wicinski, M. (2013). Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction. Molecular Medicine Reports, 8, 914-918. https://doi.org/10.3892/mmr.2013.1574
MLA
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., Wicinski, M."Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction". Molecular Medicine Reports 8.3 (2013): 914-918.
Chicago
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., Wicinski, M."Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction". Molecular Medicine Reports 8, no. 3 (2013): 914-918. https://doi.org/10.3892/mmr.2013.1574
Copy and paste a formatted citation
x
Spandidos Publications style
Szadujkis‑Szadurska K, Grzesk G, Szadujkis‑Szadurski L, Gajdus M, Malinowski B and Wicinski M: Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction. Mol Med Rep 8: 914-918, 2013.
APA
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., & Wicinski, M. (2013). Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction. Molecular Medicine Reports, 8, 914-918. https://doi.org/10.3892/mmr.2013.1574
MLA
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., Wicinski, M."Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction". Molecular Medicine Reports 8.3 (2013): 914-918.
Chicago
Szadujkis‑Szadurska, K., Grzesk, G., Szadujkis‑Szadurski, L., Gajdus, M., Malinowski, B., Wicinski, M."Role of endothelium, acetylocholine and calcium ions in Bay K8644- and KCl-induced contraction". Molecular Medicine Reports 8, no. 3 (2013): 914-918. https://doi.org/10.3892/mmr.2013.1574
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team