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September 2013 Volume 8 Issue 3

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Article

Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells

  • Authors:
    • Hye Ran Lee
    • Hyun Kyung No
    • Chun Jeih Ryu
    • Hyun-Ju Park
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Inje University Ilsan Paik Hospital, Goyang, Gyeonggi-do 411-706, Republic of Korea, School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, Republic of Korea, Department of Bioscience and Biotechnology, Sejong University, Kwanjin-Gu, Seoul 143-747, Republic of Korea
  • Pages: 747-750
    |
    Published online on: July 8, 2013
       https://doi.org/10.3892/mmr.2013.1576
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Abstract

In order to investigate the mechanism of cisplatin resistance, a cisplatin‑resistant human gastric cancer cell line was established. Subsequent to the exposure of the YCC-3 gastric cancer cell line to equal concentrations of cis‑diammine‑dichloroplatinum (II) (cisplatin, CDDP) for 6 months, a cisplatin-resistant cell line was established (YCC‑3/R). To determine the molecular mechanism of cisplatin resistance in YCC‑3/R cells, differentially expressed genes (DEGs) were investigated between YCC‑3 and YCC-3/R by annealing control primer-based reverse transcriptase-polymerase chain reaction (ACP RT‑PCR) technology. Eleven DEGs were successfully identified and sequenced. Among them, interferon‑induced transmembrane protein 1 (9‑27) and interferon α‑inducible protein 27 (IFI‑27) were markedly increased in YCC‑3/R cells. In addition, western blot analysis demonstrated that the Brahma‑related gene 1 (BRG1), which was observed to selectively activate 9‑27 and IFI‑27 genes, was overexpressed in YCC‑3/R cells. The results suggested that the BRG1‑associated expression of 9‑27 and IFI‑27 is involved in cisplatin resistance in gastric cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee HR, No HK, Ryu CJ and Park H: Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Mol Med Rep 8: 747-750, 2013.
APA
Lee, H.R., No, H.K., Ryu, C.J., & Park, H. (2013). Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Molecular Medicine Reports, 8, 747-750. https://doi.org/10.3892/mmr.2013.1576
MLA
Lee, H. R., No, H. K., Ryu, C. J., Park, H."Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells". Molecular Medicine Reports 8.3 (2013): 747-750.
Chicago
Lee, H. R., No, H. K., Ryu, C. J., Park, H."Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells". Molecular Medicine Reports 8, no. 3 (2013): 747-750. https://doi.org/10.3892/mmr.2013.1576
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HR, No HK, Ryu CJ and Park H: Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Mol Med Rep 8: 747-750, 2013.
APA
Lee, H.R., No, H.K., Ryu, C.J., & Park, H. (2013). Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Molecular Medicine Reports, 8, 747-750. https://doi.org/10.3892/mmr.2013.1576
MLA
Lee, H. R., No, H. K., Ryu, C. J., Park, H."Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells". Molecular Medicine Reports 8.3 (2013): 747-750.
Chicago
Lee, H. R., No, H. K., Ryu, C. J., Park, H."Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells". Molecular Medicine Reports 8, no. 3 (2013): 747-750. https://doi.org/10.3892/mmr.2013.1576
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