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Article

K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin

  • Authors:
    • Yu Ma
    • Fu‑Chun Guo
    • Wei Wang
    • Hua‑Shan Shi
    • Dan Li
    • Yong‑Sheng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Oncology, Cancer Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 763-768
    |
    Published online on: July 22, 2013
       https://doi.org/10.3892/mmr.2013.1596
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Abstract

An increasing number of studies support the use of metformin, a common antidiabetic drug, as a novel anticancer therapeutic. However, its mechanism of action has yet to be identified. In the current study, metformin was observed to effectively inhibit the growth of the K‑ras mutant but not wild‑type tumors in vivo. The antitumor effects of metformin were mediated by the induction of apoptosis and inhibition of proliferation in vivo. In addition, metformin induced apoptosis in the K‑ras mutant tumors, A549 and PANC‑1, but not in the K‑ras wild‑type tumor, A431, in vitro. Similarly, at lower concentrations, metformin inhibited cell proliferation in the K‑ras mutant, but not in the K‑ras wild‑type tumor cells in vitro. These observations indicate that tumors with K‑ras mutations are sensitive to metformin therapy. In addition, metformin significantly arrested K‑ras mutant and wild‑type tumor cells in G1 phase in vitro and metformin downregulated two important downstream effectors of the Ras signaling pathway in K‑ras mutant tumors. Metformin was concluded to function as a potential K‑ras‑targeting agent that has potential for cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Guo FC, Wang W, Shi HS, Li D and Wang YS: K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin. Mol Med Rep 8: 763-768, 2013.
APA
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., & Wang, Y. (2013). K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin. Molecular Medicine Reports, 8, 763-768. https://doi.org/10.3892/mmr.2013.1596
MLA
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., Wang, Y."K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin". Molecular Medicine Reports 8.3 (2013): 763-768.
Chicago
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., Wang, Y."K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin". Molecular Medicine Reports 8, no. 3 (2013): 763-768. https://doi.org/10.3892/mmr.2013.1596
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Guo FC, Wang W, Shi HS, Li D and Wang YS: K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin. Mol Med Rep 8: 763-768, 2013.
APA
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., & Wang, Y. (2013). K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin. Molecular Medicine Reports, 8, 763-768. https://doi.org/10.3892/mmr.2013.1596
MLA
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., Wang, Y."K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin". Molecular Medicine Reports 8.3 (2013): 763-768.
Chicago
Ma, Y., Guo, F., Wang, W., Shi, H., Li, D., Wang, Y."K‑ras gene mutation as a predictor of cancer cell responsiveness to metformin". Molecular Medicine Reports 8, no. 3 (2013): 763-768. https://doi.org/10.3892/mmr.2013.1596
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