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Molecular Medicine Reports
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September 2013 Volume 8 Issue 3

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Article

Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells

  • Authors:
    • Mari Mikami
    • Tomonori Sonoki
    • Minase Ito
    • Yoko Funasaka
    • Tamio Suzuki
    • Yohtaro Katagata
  • View Affiliations / Copyright

    Affiliations: Faculty of Agriculture and Life Sciences, Hirosaki University, Hirosaki, Aomori 036-8561, Japan, Nippon Medical School, Bunkyo, Tokyo 113-8602, Japan, Faculty of Medicine, Yamagata University, Yamagata 990-9585, Japan
  • Pages: 818-822
    |
    Published online on: July 26, 2013
       https://doi.org/10.3892/mmr.2013.1602
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Abstract

The majority of malignant melanoma cell types are able to produce melanin and the degree of melanin synthesis in various types of cultured cell line differs. In this study, we evaluated three types of cultured cell line, MNT‑1, HM3KO and G‑361, with differing melanin production levels. The level was greatest in the MNT‑1 cells, lower in the HM3KO cells and lowest in the G‑361 cells. In addition, a positive correlation between melanin production and tyrosinase activity was observed. The molecular masses of tyrosinases from HM3KO and G‑361 cells were marginally lower than those from MNT‑1 cells. Glycosylation inhibitor treatment on MNT‑1 cells caused decreases in the molecular mass of tyrosinase, its activity and melanin production. An immunoprecipitation assay using anti‑tyrosinase indicated that the immature glycosylated tyrosinases were associated with a type of chaperone, Hsp70. The interaction between tyrosinase and Hsp70 was also detected in HM3KO and G‑361 cells. The results indicated that the immature glycosylation of tyrosinase has a critical effect on the melanin-producing ability of melanoma cells.
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1 

Ando H, Kondoh H, Ichihashi M and Hearing VJ: Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase. J Invest Dermatol. 127:751–761. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Wang N and Hebert DN: Tyrosinase maturation through the mammalian secretory pathway: bringing color to life. Pigment Cell Res. 19:3–18. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Uong A and Zon LI: Melanocytes in development and cancer. J Cell Physiol. 222:38–41. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Kushimoto T, Basrur V, Valencia J, Matsunaga J, Vieira WD, Ferrans VJ, Muller J, Appella E and Hearing VJ: A model for melanosome biogenesis based on the purification and analysis of early melanosomes. Proc Natl Acad Sci USA. 98:10698–10703. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Watabe H, Valencia JC, Le Pape E, Yamaguchi Y, Nakamura M, Rouzaud F, Hoashi T, Kawa Y, Mizoguchi M and Hearing VJ: Involvement of dynein and spectrin with early melanosome transport and melanosomal protein trafficking. J Invest Dermatol. 128:162–174. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Godbole D, Mojamdar M and Pal JK: Increased level of p27 subunit of proteasomes and its co-localization with tyrosinase in amelanotic melanoma cells indicate its direct role in the regulation of melanin biosynthesis. Cell Biol Int. 30:895–902. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Ohashi A, Funasaka Y, Ueda M and Ichihashi M: c-KIT receptor expression in cutaneous malignant melanoma and benign melanotic naevi. Melanoma Res. 6:25–30. 1996. View Article : Google Scholar : PubMed/NCBI

8 

Ohshima Y, Yajima I, Takeda K, Iida M, Kumasaka M, Matsumoto Y and Kato M: c-RET molecule in malignant melanoma from oncogenic RET-carrying transgenic mice and human cell lines. PLoS One. 5:e102792010. View Article : Google Scholar : PubMed/NCBI

9 

Peebles PT, Trisch T and Papageorge AG: Isolation of four unusual pediatric solid tumor cell lines. Pediat Res. 12:4851978. View Article : Google Scholar

10 

Katagata Y and Hirayama T: Unexpected expression of Hsp47, a replacement of one amino acid (Val 7 Leu) in the amino terminal region, in cultured human tumorigenic cell lines. J Dermatol Sci. 49:33–38. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Ando H, Funasaka Y, Oka M, Ohashi A, Furumura M, Matsunaga J, Matsunaga N, Hearing VJ and Ichihashi M: Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis. J Lipid Res. 40:1312–1316. 1999.PubMed/NCBI

12 

Branza-Nichita N, Negroiu G, Petrescu AJ, Garman EF, Platt FM, Wormald MR, Dwek RA and Petrescu SM: Mutations at critical N-glycosylation sites reduce tyrosinase activity by altering folding and quality control. J Biol Chem. 275:8169–8175. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Asryants RA, Duszenkova IV and Nagradova NK: Determination of Sepharose-bound protein with Coomassie brilliant blue G-250. Anal Biochem. 151:571–574. 1985. View Article : Google Scholar : PubMed/NCBI

14 

Choi H, Ahn S, Chang H, Cho NS, Joo K, Lee BG, Chang I and Hwang JS: Influence of N-glycan processing disruption on tyrosinase and melanin synthesis in HM3KO melanoma cells. Exp Dermatol. 16:110–117. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Ibarrola-Villava M, Hu HH, Guedj M, Fernandez LP, Descamps V, Basset-Seguin N, Bagot M, Benssussan A, Saiag P, Fargnoli MC, et al: MC1R, SLC45A2 and TYR genetic variants involved in melanoma susceptibility in southern European populations: results from a meta-analysis. Eur J Cancer. 48:2183–2191. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Chien V, Aitken JF, Zhang S, Buchanan CM, Hickey A, Brittain T, Cooper GJ and Loomes KM: The chaperone proteins HSP70, HSP40/DnaJ and GRP78/BiP suppress misfolding and formation of β-sheet-containing aggregates by human amylin: a potential role for defective chaperone biology in Type 2 diabetes. Biochem J. 432:113–121. 2010.PubMed/NCBI

17 

Mikami K, Yamaguchi D, Tateno H, Hu D, Qin SY, Kawasaki N, Yamada M, Matsumoto N, Hirabayashi J, Ito Y and Yamamoto K: The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation. Glycobiology. 20:310–321. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mikami M, Sonoki T, Ito M, Funasaka Y, Suzuki T and Katagata Y: Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells. Mol Med Rep 8: 818-822, 2013.
APA
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., & Katagata, Y. (2013). Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells. Molecular Medicine Reports, 8, 818-822. https://doi.org/10.3892/mmr.2013.1602
MLA
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., Katagata, Y."Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells". Molecular Medicine Reports 8.3 (2013): 818-822.
Chicago
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., Katagata, Y."Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells". Molecular Medicine Reports 8, no. 3 (2013): 818-822. https://doi.org/10.3892/mmr.2013.1602
Copy and paste a formatted citation
x
Spandidos Publications style
Mikami M, Sonoki T, Ito M, Funasaka Y, Suzuki T and Katagata Y: Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells. Mol Med Rep 8: 818-822, 2013.
APA
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., & Katagata, Y. (2013). Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells. Molecular Medicine Reports, 8, 818-822. https://doi.org/10.3892/mmr.2013.1602
MLA
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., Katagata, Y."Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells". Molecular Medicine Reports 8.3 (2013): 818-822.
Chicago
Mikami, M., Sonoki, T., Ito, M., Funasaka, Y., Suzuki, T., Katagata, Y."Glycosylation of tyrosinase is a determinant of melanin production in cultured melanoma cells". Molecular Medicine Reports 8, no. 3 (2013): 818-822. https://doi.org/10.3892/mmr.2013.1602
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