Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October 2013 Volume 8 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2013 Volume 8 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats

  • Authors:
    • Peng Gong
    • Yingxin Wang
    • Jing Zhang
    • Zhongyu Wang
  • View Affiliations / Copyright

    Affiliations: Hepatobiliary Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 110001, P.R. China
  • Pages: 1005-1010
    |
    Published online on: July 31, 2013
       https://doi.org/10.3892/mmr.2013.1606
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Stem cell‑derived hepatocyte precursor cells represent a promising model for clinical transplantation to diseased livers, as well as for establishment of in vitro systems for drug metabolism and toxicology studies. The present study aimed to establish a new method of induction of hepatocyte differentiation using various factors and evaluate the effect of different partial hepatectomies and the duration of collagenase perfusion on hepatic stem cell proliferation and differentiation. A rat model of hepatic oval cell proliferation was established by partial hepatectomy (PH). Following 73.1 and 83.4% PH, rats underwent perfusion with IV collagenase for 10, 20 and 30 min. Density gradient centrifugation was performed and cells in the supernatant were cultured in various combinations of factors to induce oval cells to differentiate into mature hepatocytes. Cells were characterized for hepatocyte marker expression by morphology, flow cytometry, immunofluorescence and western blot analysis. Hepatic oval cells isolated from rats at 7 and 14 days post‑PH exhibited properties of hepatic stem/progenitor cells. Following culturing in RPMI‑1640 medium with hepatocyte growth factor and fibroblast growth factor‑4, the cells resembled primary human hepatocytes with regard to morphology and expression of the hepatocyte markers, cytokeratin 18 (CK‑18) and α‑1‑fetoprotein (AFP). Optimal differentiation of hepatic stem cells to CK‑18‑ and AFP‑positive cells was observed when stem cells isolated from 83.4% PH rats (7 days following surgery) were perfused with IV collagenase for 20 min. The results of this study provide novel insights into characteristics of rat hepatic stem cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kim WR, Therneau TM, Benson JT, et al: Deaths on the liver transplant waiting list: an analysis of competing risks. Hepatology. 43:345–351. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Duan BW, Lu SC, Wang ML, et al: Liver transplantation in acute-on-chronic liver failure patients with high model for end-stage liver disease (MELD) scores: a single center experience of 100 consecutive cases. J Surg Res. 183:936–943. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Verfaillie CM, Pera MF and Lansdorp PM: Stem cells: hype and reality. Hematology Am Soc Hematol Educ Program. 2002:369–391. 2002. View Article : Google Scholar

4 

Fiegel HC, Lange C, Kneser U, et al: Fetal and adult liver stem cells for liver regeneration and tissue engineering. J Cell Mol Med. 10:577–587. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Alison MR, Choong C and Lim S: Application of liver stem cells for cell therapy. Semin Cell Dev Biol. 18:819–826. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Wilson JW and Leduc EH: Role of cholangioles in restoration of the liver of the mouse after dietary injury. J Pathol Bacteriol. 76:441–449. 1958. View Article : Google Scholar : PubMed/NCBI

7 

Arber N, Zajicek G and Ariel I: The streaming liver. II. Hepatocyte life history. Liver. 8:80–87. 1988. View Article : Google Scholar : PubMed/NCBI

8 

Haruna Y, Saito K, Spaulding S, Nalesnik MA and Gerber MA: Identification of bipotential progenitor cells in human liver development. Hepatology. 23:476–481. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Petersen BE, Bowen WC, Patrene KD, et al: Bone marrow as a potential source of hepatic oval cells. Science. 284:1168–1170. 1999.PubMed/NCBI

10 

Wang X, Foster M, Al-Dhalimy M, et al: The origin and liver repopulating capacity of murine oval cells. Proc Natl Acad Sci USA. 100(Suppl 1): 11881–11888. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Li J, Li M, Niu B and Gong J: Therapeutic potential of stem cell in liver regeneration. Front Med. 5:26–32. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Takami T, Terai S and Sakaida I: Stem cell therapy in chronic liver disease. Curr Opin Gastroenterol. 28:203–208. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Russo FP and Parola M: Stem and progenitor cells in liver regeneration and repair. Cytotherapy. 13:135–144. 2011. View Article : Google Scholar : PubMed/NCBI

14 

He ZP, Tan WQ, Tang YF, Zhang HJ and Feng MF: Activation, isolation, identification and in vitro proliferation of oval cells from adult rat livers. Cell Prolif. 37:177–187. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Colletti LM, Green M, Burdick MD, Kunkel SL and Strieter RM: Proliferative effects of CXC chemokines in rat hepatocytes in vitro and in vivo. Shock. 10:248–257. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Higgins GM and Anderson RM: Restoration of the liver of the white rat following partial surgical removal. Arch Pathol. 12:186–202. 1931.

17 

Shupe TD, Piscaglia AC, Oh SH, Gasbarrini A and Petersen BE: Isolation and characterization of hepatic stem cells, or ‘oval cells’, from rat livers. Methods Mol Biol. 482:387–405. 2009.

18 

Pack R, Heck R, Dienes HP, Oesch F and Steinberg P: Isolation, biochemical characterization, long-term culture and phenotype modulation of oval cells from carcinogen-fed rats. Exp Cell Res. 204:198–209. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Bussolati B, Deambrosis I, Russo S, et al: Altered angiogenesis and survival in humantumor-derived endothelial cells. FASEB J. 17:1159–1161. 2003.PubMed/NCBI

20 

Herrera MB, Bruno S, Buttiglieri S, et al: Isolation and characterization of a stem cell population from adult human liver. Stem Cells. 24:2840–2850. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Lazaro CA, Rhim JA, Yamada Y and Fausto N: Generation of hepatocytes from oval cell precursors in culture. Cancer Res. 58:5514–5522. 1998.PubMed/NCBI

22 

Rogler LE: Selective bipotential differentiation of mouse embryonic hepatoblasts in vitro. Am J Pathol. 150:591–602. 1997.PubMed/NCBI

23 

Czyz J, Wiese C, Rolletschek A, et al: Potential of embryonic and adult stem cells in vitro. Biol Chem. 384:1391–1409. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Oh SH, Miyazaki M, Kouchi H, et al: Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro. Biochem Biophys Res Commun. 279:500–504. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Fiegel HC, Lioznov MV, Cortes-Dericks, et al: Liver-specific gene expression in cultured human hematopoietic stem cells. Stem Cells. 21:98–104. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Miyazaki M, Akiyama I, Sakaguchi M, et al: Improved conditions to induce hepatocytes from rat bone marrow cells in culture. Biochem Biophys Res Commun. 298:24–30. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Schwartz RE, Reyes M, Koodie L, et al: Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte like cells. J Clin Invest. 109:1291–1302. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Block GD, Locker J, Bowen WC, et al: Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGF alpha in a chemically defined (HGM) medium. J Cell Biol. 132:1133–1149. 1996. View Article : Google Scholar

29 

Michalopoulos GK, Bowen WC, Mule K, et al: HGF-, EGF- and dexamethas-one-induced gene expression patterns during formation of tissue in hepatic organoid cultures. Gene Expr. 11:55–75. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Jang YY, Collector MI, Baylin SB, et al: Hematopoietic stem cells convert into liver cells within days without fusion. Nat Cell Biol. 6:532–539. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Ruhnke M, Nussler AK, Ungefroren H, et al: Human monocyte-derived neohepatocytes: a promising alternative to primary human hepatocytes for autologous cell therapy. Transplantation. 79:1097–1103. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Ruhnke M, Ungefroren H, Nussler AK, et al: Differentiation of in vitro-modified human peripheral blood monocytes into hepatocyte-like and pancreatic islet-like cells. Gastroenterology. 128:1774–1786. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Terrace JD, Currie IS, Hay DC, et al: Progenitor cell characterization and location in the developing human liver. Stem Cells Dev. 16:771–778. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Petersen BE, Goff JP, Greenberger JS and Michalopoulos GK: Hepatic oval cells express the hematopoietic stem cell marker Thy-1 in the rat. Hepatology. 27:433–445. 1998. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gong P, Wang Y, Zhang J and Wang Z: Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats. Mol Med Rep 8: 1005-1010, 2013.
APA
Gong, P., Wang, Y., Zhang, J., & Wang, Z. (2013). Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats. Molecular Medicine Reports, 8, 1005-1010. https://doi.org/10.3892/mmr.2013.1606
MLA
Gong, P., Wang, Y., Zhang, J., Wang, Z."Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats". Molecular Medicine Reports 8.4 (2013): 1005-1010.
Chicago
Gong, P., Wang, Y., Zhang, J., Wang, Z."Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats". Molecular Medicine Reports 8, no. 4 (2013): 1005-1010. https://doi.org/10.3892/mmr.2013.1606
Copy and paste a formatted citation
x
Spandidos Publications style
Gong P, Wang Y, Zhang J and Wang Z: Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats. Mol Med Rep 8: 1005-1010, 2013.
APA
Gong, P., Wang, Y., Zhang, J., & Wang, Z. (2013). Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats. Molecular Medicine Reports, 8, 1005-1010. https://doi.org/10.3892/mmr.2013.1606
MLA
Gong, P., Wang, Y., Zhang, J., Wang, Z."Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats". Molecular Medicine Reports 8.4 (2013): 1005-1010.
Chicago
Gong, P., Wang, Y., Zhang, J., Wang, Z."Differential hepatic stem cell proliferation and differentiation after partial hepatectomy in rats". Molecular Medicine Reports 8, no. 4 (2013): 1005-1010. https://doi.org/10.3892/mmr.2013.1606
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team