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October 2013 Volume 8 Issue 4

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Article

Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB

  • Authors:
    • Jie Zhu
    • Yuanyuan Zhang
    • Yueshuang Shen
    • Huanqin Zhou
    • Xiaomei Yu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Respiratory Medicine, Children's Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 1216-1220
    |
    Published online on: July 31, 2013
       https://doi.org/10.3892/mmr.2013.1608
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Abstract

Dendritic cells (DCs) are potent antigen‑presenting cells that play pivotal roles in the initiation of primary immune responses. Lycium barbarum polysaccharides (LBPs) are known to have a variety of immunomodulatory functions. However, the cellular and molecular mechanisms underlying their therapeutic effects are poorly understood. In this study, we report that LBPs induce phenotypic and functional maturation of DCs. LBPs upregulated DC expression of I‑A/I‑E and CD11c, enhanced DC allostimulatory activity and induced IL‑12p40 production. Furthermore, the activity of LBPs on DCs was significantly reduced by treating the cells with anti‑TLR2 or anti‑TLR4 antibody prior to LBPs, indicating that both are possible receptors of LBPs. Maturation of DCs by LBPs was able to directly activate the nuclear transcription factor NF‑κB p65. The results revealed that LBP stimulation induces the phenotypic and functional maturation of DCs via TLR2- and/or TLR4-mediated NF‑κB signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Zhang Y, Shen Y, Zhou H and Yu X: Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB. Mol Med Rep 8: 1216-1220, 2013.
APA
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., & Yu, X. (2013). Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB. Molecular Medicine Reports, 8, 1216-1220. https://doi.org/10.3892/mmr.2013.1608
MLA
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., Yu, X."Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB". Molecular Medicine Reports 8.4 (2013): 1216-1220.
Chicago
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., Yu, X."Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB". Molecular Medicine Reports 8, no. 4 (2013): 1216-1220. https://doi.org/10.3892/mmr.2013.1608
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Zhang Y, Shen Y, Zhou H and Yu X: Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB. Mol Med Rep 8: 1216-1220, 2013.
APA
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., & Yu, X. (2013). Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB. Molecular Medicine Reports, 8, 1216-1220. https://doi.org/10.3892/mmr.2013.1608
MLA
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., Yu, X."Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB". Molecular Medicine Reports 8.4 (2013): 1216-1220.
Chicago
Zhu, J., Zhang, Y., Shen, Y., Zhou, H., Yu, X."Lycium barbarum polysaccharides induce Toll‑like receptor 2‑ and 4‑mediated phenotypic and functional maturation of murine dendritic cells via activation of NF‑κB". Molecular Medicine Reports 8, no. 4 (2013): 1216-1220. https://doi.org/10.3892/mmr.2013.1608
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