Effects of electroacupuncture on spinal α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor in rats injected with complete Freund's adjuvant

Lee,Young-Seok; Lee,Jun-Hyuk; Lee,In-Seon; Choi,Byung-Tae

doi:10.3892/mmr.2013.1633

October 2013 Volume 8 Issue 4

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October 2013 Volume 8 Issue 4

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Article

Effects of electroacupuncture on spinal α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor in rats injected with complete Freund's adjuvant

Authors:
- Young-Seok Lee
- Jun-Hyuk Lee
- In-Seon Lee
- Byung-Tae Choi
View Affiliations / Copyright

Affiliations: Department of Rehabilitation, College of Oriental Medicine, Dong-eui University, Busanjn‑gu, Busan 614-052, Republic of Korea, Chemistry and Biotechnology Examination Bureau, Korean Intellectual Property Office, Seo‑gu, Daejeon 302-701, Republic of Korea, Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 626-870, Republic of Korea
Pages: 1130-1134
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Published online on: August 14, 2013

https://doi.org/10.3892/mmr.2013.1633
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Abstract

The effects of electroacupuncture (EA) on spinal α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptor subunits in male rats injected with complete Freund's adjuvant (CFA) were investigated. Bilateral EA stimulation (2 Hz, 1 mA) was administered by needle insertion for 30 min once daily at acupoints corresponding to Zusanli and Sanyinjiao, and the thermal thresholds were measured. To examine the changes in the AMPA receptor subunits, the L4-5 segments of the spinal cord were analyzed by qPCR, western blot analysis and immunohistochemistry. The CFA-induced thermal sensitivity of the rat hind paw was significantly inhibited by EA stimulation from day 3 following CFA injection. On day 5 following CFA injection, there were no significant changes in the expression of the AMPA receptor GluR1 subunit in the CFA-injected rats with or without EA stimulation, compared with the control rats. However, the expression of the GluR2 subunit was significantly decreased by CFA treatment. Western blot analysis revealed that the expression of the phosphorylated GluR1 subunit in the control rats was not significantly different compared with that in the CFA-injected rats with and without EA stimulation. However, phosphorylation of the ipsilateral GluR2 subunit was significantly increased in the CFA-injected rats, and this activation was prevented by EA stimulation. Immunohistochemical analysis revealed a greater expression of phospho-GluR2 following CFA injection, which was inhibited by EA stimulation. These results suggested that phosphorylation of the AMPA receptor, particularly the GluR2 subunit, may be important in EA analgesia of CFA-induced inflammation.

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1	Collingridge GL, Isaac JT and Wang YT: Receptor trafficking and synaptic plasticity. Nat Rev Neurosci. 5:952–962. 2004. View Article : Google Scholar
2	Gao X, Kim HK, Chung JM and Chung K: Enhancement of NMDA receptor phosphorylation of the spinal dorsal horn and nucleus gracilis neurons in neuropathic rats. Pain. 116:62–72. 2005. View Article : Google Scholar : PubMed/NCBI
3	Katano T, Furue H, Okuda-Ashitaka E, et al: N-ethylmaleimide- sensitive fusion protein (NSF) is involved in central sensitization in the spinal cord through GluR2 subunit composition switch after inflammation. Eur J Neurosci. 27:3161–3170. 2008. View Article : Google Scholar
4	Burnashev N, Khodorova A, Jonas P, et al: Calcium-permeable AMPA-kainate receptors in fusiform cerebellar glial cells. Science. 256:1566–1570. 1992. View Article : Google Scholar : PubMed/NCBI
5	Zhou QQ, Imbe H, Zou S, Dubner R and Ren K: Selective upregulation of the flip-flop splice variants of AMPA receptor subunits in the rat spinal cord after hindpaw inflammation. Brain Res Mol Brain Res. 88:186–193. 2001. View Article : Google Scholar : PubMed/NCBI
6	Park JS, Voitenko N, Petralia RS, et al: Persistent inflammation induces GluR2 internalization via NMDA receptor-triggered PKC activation in dorsal horn neurons. J Neurosci. 29:3206–3219. 2009. View Article : Google Scholar : PubMed/NCBI
7	Kerr RC, Maxwell DJ and Todd AJ: GluR1 and GluR2/3 subunits of the AMPA-type glutamate receptor are associated with particular types of neurone in laminae I–III of the spinal dorsal horn of the rat. Eur J Neurosci. 10:324–333. 1998.PubMed/NCBI
8	Lu Y, Sun YN, Wu X, et al: Role of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR1 in spinal dorsal horn in inflammatory nociception and neuropathic nociception in rat. Brain Res. 1200:19–26. 2008. View Article : Google Scholar
9	Park JS, Yaster M, Guan X, et al: Role of spinal cord alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors in complete Freund’s adjuvant-induced inflammatory pain. Mol Pain. 4:672008.PubMed/NCBI
10	Choi BT, Lee JH, Wan Y and Han JS: Involvement of ionotropic glutamate receptors in low frequency electroacupuncture analgesia in rats. Neurosci Lett. 377:185–188. 2005. View Article : Google Scholar : PubMed/NCBI
11	Melzack R, Vetere P and Finch L: Transcutaneous electrical nerve stimulation for low back pain. A comparison of TENS and massage for pain and range of motion. Phys Ther. 63:489–493. 1983.PubMed/NCBI
12	Chen J and Sandkühler J: Induction of homosynaptic long-term depression at spinal synapses of sensory a delta-fibres requires activation of metabotropic glutamate receptors. Neuroscience. 98:141–148. 2000. View Article : Google Scholar : PubMed/NCBI
13	Pellegrini-Giampietro DE, Fan S, Ault B, Miller BE and Zukin RS: Glutamate receptor gene expression in spinal cord of arthritic rats. J Neurosci. 14:1576–1583. 1994.PubMed/NCBI
14	Guan Y, Guo W, Zou SP, Dubner R and Ren K: Inflammation- induced upregulation of AMPA receptor subunit expression in brain stem pain modulatory circuitry. Pain. 104:401–413. 2003. View Article : Google Scholar : PubMed/NCBI
15	Lee J and Ro JY: Differential regulation of glutamate receptors in trigeminal ganglia following masseter inflammation. Neurosci Lett. 421:91–95. 2007. View Article : Google Scholar : PubMed/NCBI
16	Garry EM and Fleetwood-Walker SM: A new view on how AMPA receptors and their interacting proteins mediate neuropathic pain. Pain. 109:210–213. 2004. View Article : Google Scholar : PubMed/NCBI
17	Nagy GG, Al-Ayyan M, Andrew D, Fukaya M, Watanabe M and Todd AJ: Widespread expression of the AMPA receptor GluR2 subunit at glutamatergic synapses in the rat spinal cord and phosphorylation of GluR1 in response to noxious stimulation revealed with an antigen- unmasking method. J Neurosci. 24:5766–5777. 2004. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of electroacupuncture on spinal α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor in rats injected with complete Freund's adjuvant

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