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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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October 2013 Volume 8 Issue 4

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro

  • Authors:
    • Lin Yang
    • Xiao-Hou Wu
    • Dan Wang
    • Chun-Li Luo
    • Li-Xue Chen
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China, Department of Laboratory Diagnostics, College of Laboratory Medicine, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China, Experimental Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Yuzhong, Chongqing 400016, P.R. China
  • Pages: 1272-1278
    |
    Published online on: August 14, 2013
       https://doi.org/10.3892/mmr.2013.1634
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Abstract

Exosomes are small membrane vesicles released by a variety of mammalian cells into the extracellular space and are involved in cell‑to‑cell signaling. This study aimed to investigate the effects of bladder cancer cell‑derived exosomes on the regulation of tumor cell viability and apoptosis, as well as the underlying molecular events. Exosomes were purified from the supernatants of human bladder cancer T24 cell cultures. Transmission electron microscopy was used to confirm their morphology and western blot analyses determined the protein content of cells. Subsequently, bladder cancer cell lines were treated with different concentrations of exosomes. Tumor cell viability was shown to be reduced, as detected by the Cell Counting Kit‑8 assay. Annexin V/flow cytometric assays showed that exosomes inhibited apoptosis of bladder cancer cell lines in a dose- and time‑dependent manner. Exosomes were demonstrated to upregulate the expression of Bcl‑2 and Cyclin D1 proteins, but reduce the levels of Bax and caspase‑3 proteins in these cells. Moreover, exosomes dose‑dependently increased the expression of phosphorylated Akt and extracellular signal‑regulated protein kinase (ERK). In conclusion, this study demonstrated that bladder cancer cell‑derived exosomes inhibited tumor cell apoptosis, which was associated with the activation of Akt and ERK pathway genes, suggesting that tumor‑derived exosomes are involved in bladder cancer progression. Inhibition of exosome formation and release may therefore be a novel strategy in future treatment of bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Wu X, Wang D, Luo C and Chen L: Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro. Mol Med Rep 8: 1272-1278, 2013.
APA
Yang, L., Wu, X., Wang, D., Luo, C., & Chen, L. (2013). Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro. Molecular Medicine Reports, 8, 1272-1278. https://doi.org/10.3892/mmr.2013.1634
MLA
Yang, L., Wu, X., Wang, D., Luo, C., Chen, L."Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro". Molecular Medicine Reports 8.4 (2013): 1272-1278.
Chicago
Yang, L., Wu, X., Wang, D., Luo, C., Chen, L."Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro". Molecular Medicine Reports 8, no. 4 (2013): 1272-1278. https://doi.org/10.3892/mmr.2013.1634
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Wu X, Wang D, Luo C and Chen L: Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro. Mol Med Rep 8: 1272-1278, 2013.
APA
Yang, L., Wu, X., Wang, D., Luo, C., & Chen, L. (2013). Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro. Molecular Medicine Reports, 8, 1272-1278. https://doi.org/10.3892/mmr.2013.1634
MLA
Yang, L., Wu, X., Wang, D., Luo, C., Chen, L."Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro". Molecular Medicine Reports 8.4 (2013): 1272-1278.
Chicago
Yang, L., Wu, X., Wang, D., Luo, C., Chen, L."Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro". Molecular Medicine Reports 8, no. 4 (2013): 1272-1278. https://doi.org/10.3892/mmr.2013.1634
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