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Article

Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis

  • Authors:
    • Jia-Le Song
    • Yu Qian
    • Gui-Jie Li
    • Xin Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
  • Pages: 1256-1262
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    Published online on: August 14, 2013
       https://doi.org/10.3892/mmr.2013.1635
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Abstract

The present study aimed to investigate the anti‑inflammatory effects of Ilex kudingcha C.J. Tseng methanol extracts (KME) on 3% dextran sulfate sodium (DSS)‑induced ulcerative colitis (UC) in mice (C57BL/6J strain). Body weight, disease activity index (DAI), colon length, colon weight to length ratio, colonic myeloperoxidase (MPO), glutathione (GSH) and malondialdehyde (MDA) levels were measured. Histological changes were observed by hematoxylin and eosin staining. Colonic levels of tumor necrosis factor‑α (TNF‑α), interleukin(IL)‑1β and IL‑6 were measured with an enzyme‑linked immunosorbent assay. The mRNA expression of TNF‑α, IL‑1β, ‑6, inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 (COX-2) in the colon tissue, was quantified by RT‑PCR. KME significantly suppressed DSS‑induced body weight loss, colon length shortening and decreased the colon weight to length ratio. It also resulted in increased GSH and reduced MPO and MDA levels in the colon tissue. Histological observation suggested that KME prevented edema, mucosal damage and loss of crypts, which are induced by DSS. In addition, KME decreased the levels of TNF‑α, IL‑1β and ‑6 in the colon tissues, while inhibiting the mRNA expression of these cytokines, as well as iNOS and COX‑2. The results of this study suggested that KME has anti‑inflammatory effects on DSS‑induced UC in mice (C57BL/6J strain) by reducing the colonic levels and inhibiting the mRNA expression of pro‑inflammatory cytokines.
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Copy and paste a formatted citation
Spandidos Publications style
Song J, Qian Y, Li G and Zhao X: Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis. Mol Med Rep 8: 1256-1262, 2013.
APA
Song, J., Qian, Y., Li, G., & Zhao, X. (2013). Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis. Molecular Medicine Reports, 8, 1256-1262. https://doi.org/10.3892/mmr.2013.1635
MLA
Song, J., Qian, Y., Li, G., Zhao, X."Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis". Molecular Medicine Reports 8.4 (2013): 1256-1262.
Chicago
Song, J., Qian, Y., Li, G., Zhao, X."Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis". Molecular Medicine Reports 8, no. 4 (2013): 1256-1262. https://doi.org/10.3892/mmr.2013.1635
Copy and paste a formatted citation
x
Spandidos Publications style
Song J, Qian Y, Li G and Zhao X: Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis. Mol Med Rep 8: 1256-1262, 2013.
APA
Song, J., Qian, Y., Li, G., & Zhao, X. (2013). Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis. Molecular Medicine Reports, 8, 1256-1262. https://doi.org/10.3892/mmr.2013.1635
MLA
Song, J., Qian, Y., Li, G., Zhao, X."Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis". Molecular Medicine Reports 8.4 (2013): 1256-1262.
Chicago
Song, J., Qian, Y., Li, G., Zhao, X."Anti-inflammatory effects of kudingcha methanol extract (Ilex kudingcha C.J. Tseng) in dextran sulfate sodium-induced ulcerative colitis". Molecular Medicine Reports 8, no. 4 (2013): 1256-1262. https://doi.org/10.3892/mmr.2013.1635
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