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Article

Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone

  • Authors:
    • Wing Sum Siu
    • Chun Hay Ko
    • Leung Kim Hung
    • Ching Po Lau
    • Clara Bik San Lau
    • Kwok Pui Fung
    • Ping Chung Leung
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P.R. China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, P.R. China
  • Pages: 1188-1194
    |
    Published online on: August 20, 2013
       https://doi.org/10.3892/mmr.2013.1647
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Abstract

Pharmaceutical countermeasures to treat disuse osteoporosis are rarely studied. Pharmaceutical studies for the treatment and prevention of osteoporosis depend on the ovariectomized rat model, which is a suitable model for the disease in women. Disuse osteoporosis affects men and women, but there is lack of awareness and relevant pharmaceutical studies for this condition. The objectives of this study were to verify the validity of an unusual tail-suspension rat model in the induction of disuse osteoporosis and subsequent pharmaceutical treatments. This model was created by unloading the hind limbs of the rats in order to create a state of weightlessness in their hindlimb bones. Validation of the model was performed with non-suspended rats. This study included five groups of suspended rats fed with different agents, such as distilled water (control), high-, medium- and low-dose raloxifene and a bisphosphonate (alendronate). The experiment lasted for 28 days. Comparisons were made between the suspended control and treatment groups. Ovariectomized and sham‑operated rats were also included as a reference for bone changes during osteoporosis. Changes in bone mineral density (BMD) at the distal femur and proximal tibia, microarchitecture at the distal femur and biomechanical strength at the diaphyseal femur were studied. Reduction of BMD and deterioration of trabeculae were similar between the suspended control and ovariectomized rats. Loss of BMD induced by tail suspension was reduced most effectively by medium-dose raloxifene. Deterioration of trabecular microarchitecture was also prevented by raloxifene. The tail-suspension rat model is suitable for the study of disuse osteoporosis under the effects of various therapeutic agents. The preventive effects of raloxifene against bone loss under disuse conditions have been demonstrated using this model.
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Copy and paste a formatted citation
Spandidos Publications style
Siu WS, Ko CH, Hung LK, Lau CP, Lau CB, Fung KP and Leung PC: Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone. Mol Med Rep 8: 1188-1194, 2013.
APA
Siu, W.S., Ko, C.H., Hung, L.K., Lau, C.P., Lau, C.B., Fung, K.P., & Leung, P.C. (2013). Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone. Molecular Medicine Reports, 8, 1188-1194. https://doi.org/10.3892/mmr.2013.1647
MLA
Siu, W. S., Ko, C. H., Hung, L. K., Lau, C. P., Lau, C. B., Fung, K. P., Leung, P. C."Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone". Molecular Medicine Reports 8.4 (2013): 1188-1194.
Chicago
Siu, W. S., Ko, C. H., Hung, L. K., Lau, C. P., Lau, C. B., Fung, K. P., Leung, P. C."Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone". Molecular Medicine Reports 8, no. 4 (2013): 1188-1194. https://doi.org/10.3892/mmr.2013.1647
Copy and paste a formatted citation
x
Spandidos Publications style
Siu WS, Ko CH, Hung LK, Lau CP, Lau CB, Fung KP and Leung PC: Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone. Mol Med Rep 8: 1188-1194, 2013.
APA
Siu, W.S., Ko, C.H., Hung, L.K., Lau, C.P., Lau, C.B., Fung, K.P., & Leung, P.C. (2013). Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone. Molecular Medicine Reports, 8, 1188-1194. https://doi.org/10.3892/mmr.2013.1647
MLA
Siu, W. S., Ko, C. H., Hung, L. K., Lau, C. P., Lau, C. B., Fung, K. P., Leung, P. C."Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone". Molecular Medicine Reports 8.4 (2013): 1188-1194.
Chicago
Siu, W. S., Ko, C. H., Hung, L. K., Lau, C. P., Lau, C. B., Fung, K. P., Leung, P. C."Effect of anti-osteoporotic agents on the prevention of bone loss in unloaded bone". Molecular Medicine Reports 8, no. 4 (2013): 1188-1194. https://doi.org/10.3892/mmr.2013.1647
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