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Article

Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression

  • Authors:
    • Lin Wang
    • Guiying Wang
    • Dandan Yang
    • Xudong Guo
    • Yanxin Xu
    • Bo Feng
    • Jiuhong Kang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, East Hospital, Tongji University School of Medicine, Yangpu, Shanghai 200092, P.R. China, Clinical and Translational Research Center of Shanghai First Maternity and Infant Health Hospital, Tongji University, Yangpu, Shanghai 200092, P.R. China
  • Pages: 1279-1285
    |
    Published online on: August 22, 2013
       https://doi.org/10.3892/mmr.2013.1650
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Abstract

Euphorbia tirucalli is a long‑established treatment for a wide variety of cancers. However, the mechanism of its anticancer effect is yet to be elucidated. In the present study, we examined the anticancer effect of euphol, a tetracyclic triterpene alcohol isolated from the sap of Euphorbia tirucalli, in T47D human breast cancer cells. Following the treatment of cells with different doses of euphol for 24, 48 and 72 h, the cell proliferation, cell cycle, and mRNA and protein levels of cell cycle regulatory molecules were analyzed, respectively. Treatment of the cells with euphol resulted in decreased cell viability, which was accompanied by an accumulation of cells in the G1 phase. Further studies demonstrated that euphol treatment downregulated cyclin D1 expression and the hypophosphorylation of Rb. Furthermore, this effect was correlated with the downregulation of cyclin‑dependent kinase 2 (CDK2) expression and the upregulation of the CDK inhibitors p21 and p27. Reduced expression levels of cyclin A and B1 were also observed, corresponding to the decreased distribution of cells in the S and G2/M phases, respectively. These findings indicated that euphol is an active agent in Euphorbia tirucalli that exerts anticancer activity by arresting the cell cycle of cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Wang G, Yang D, Guo X, Xu Y, Feng B and Kang J: Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression. Mol Med Rep 8: 1279-1285, 2013.
APA
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., & Kang, J. (2013). Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression. Molecular Medicine Reports, 8, 1279-1285. https://doi.org/10.3892/mmr.2013.1650
MLA
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., Kang, J."Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression". Molecular Medicine Reports 8.4 (2013): 1279-1285.
Chicago
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., Kang, J."Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression". Molecular Medicine Reports 8, no. 4 (2013): 1279-1285. https://doi.org/10.3892/mmr.2013.1650
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Wang G, Yang D, Guo X, Xu Y, Feng B and Kang J: Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression. Mol Med Rep 8: 1279-1285, 2013.
APA
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., & Kang, J. (2013). Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression. Molecular Medicine Reports, 8, 1279-1285. https://doi.org/10.3892/mmr.2013.1650
MLA
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., Kang, J."Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression". Molecular Medicine Reports 8.4 (2013): 1279-1285.
Chicago
Wang, L., Wang, G., Yang, D., Guo, X., Xu, Y., Feng, B., Kang, J."Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression". Molecular Medicine Reports 8, no. 4 (2013): 1279-1285. https://doi.org/10.3892/mmr.2013.1650
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