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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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2014-January Volume 9 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering

  • Authors:
    • Jun Zou
    • Chenxi Yuan
    • Chunshen Wu
    • Cheng Cao
    • Qing Shi
    • Huilin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
  • Pages: 185-191
    |
    Published online on: October 25, 2013
       https://doi.org/10.3892/mmr.2013.1758
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Abstract

The purpose of this study was to investigate the isolation and culture of muscle‑derived stem cells (MDSCs) and their capability to differentiate into osteoblasts in vitro. Skeletal muscle tissue was obtained from double hind limbs of New Zealand white rabbits under sterile conditions and isolated by collagenase digestion. Following passages in basic medium, the primary cells were desmin (+), myosin (+) and CD105 (+). Differentiation of MDSCs was induced by osteogenic medium. Using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide assay, the differentiated cell population was found to proliferate faster than the undifferentiated. Alkaline phosphatase staining and alizarin red staining revealed that the differentiated cells were mineralized in vitro. Quantitative polymerase chain reaction assays also showed increased mRNA expression of osteogenic genes in differentiated cells. In conclusion, stem cells were successfully isolated and cultured from rabbit skeletal muscle tissue and were able to differentiate into osteoblasts following induction. These observations may indicate an ideal stem cell source for tissue engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Zou J, Yuan C, Wu C, Cao C, Shi Q and Yang H: Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering. Mol Med Rep 9: 185-191, 2014.
APA
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., & Yang, H. (2014). Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering. Molecular Medicine Reports, 9, 185-191. https://doi.org/10.3892/mmr.2013.1758
MLA
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., Yang, H."Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering". Molecular Medicine Reports 9.1 (2014): 185-191.
Chicago
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., Yang, H."Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering". Molecular Medicine Reports 9, no. 1 (2014): 185-191. https://doi.org/10.3892/mmr.2013.1758
Copy and paste a formatted citation
x
Spandidos Publications style
Zou J, Yuan C, Wu C, Cao C, Shi Q and Yang H: Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering. Mol Med Rep 9: 185-191, 2014.
APA
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., & Yang, H. (2014). Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering. Molecular Medicine Reports, 9, 185-191. https://doi.org/10.3892/mmr.2013.1758
MLA
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., Yang, H."Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering". Molecular Medicine Reports 9.1 (2014): 185-191.
Chicago
Zou, J., Yuan, C., Wu, C., Cao, C., Shi, Q., Yang, H."Isolation and osteogenic differentiation of skeletal muscle‑derived stem cells for bone tissue engineering". Molecular Medicine Reports 9, no. 1 (2014): 185-191. https://doi.org/10.3892/mmr.2013.1758
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