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Article

Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways

  • Authors:
    • Li-Jiang Tao
    • Xin-Die Zhou
    • Cheng-Chun Shen
    • Cheng-Zhen Liang
    • Bing Liu
    • Yiqing Tao
    • Hui-Min Tao
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China
  • Pages: 345-349
    |
    Published online on: October 29, 2013
       https://doi.org/10.3892/mmr.2013.1761
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Abstract

Although neoadjuvant chemotherapy has improved the survival rate of osteosarcoma patients, drug resistance remains a predominant obstacle to improving efficacy and necessitates the development of novel chemotherapeutical agents. The aim of this study was to investigate whether tetrandrine (TET) induces apoptosis in the U-2OS and MG-63 osteosarcoma cell lines and to further determine the underlying mechanism. This study investigated the effects of TET on osteosarcoma in vitro. To examine the antitumor effects of TET on osteosarcoma, the two osteosarcoma cell lines were treated with TET and subjected to apoptosis assays. The results revealed that TET induced the apoptosis of osteosarcoma cells in a time- and dose-dependent manner. Furthermore, the apoptosis of osteosarcoma cells was accompanied by increased cytochrome c (Cyto-C), apoptotic protease-activating factor (Apaf)-1, Bid and Bax activation and reduced Bcl-2 and Bcl-xl activation, demonstrating that the apoptosis may have occurred through the mitochondrial pathway. In conclusion, the results suggest that TET is a promising agent for osteosarcoma therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Tao L, Zhou X, Shen C, Liang C, Liu B, Tao Y and Tao H: Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways. Mol Med Rep 9: 345-349, 2014.
APA
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., & Tao, H. (2014). Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways. Molecular Medicine Reports, 9, 345-349. https://doi.org/10.3892/mmr.2013.1761
MLA
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., Tao, H."Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways". Molecular Medicine Reports 9.1 (2014): 345-349.
Chicago
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., Tao, H."Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways". Molecular Medicine Reports 9, no. 1 (2014): 345-349. https://doi.org/10.3892/mmr.2013.1761
Copy and paste a formatted citation
x
Spandidos Publications style
Tao L, Zhou X, Shen C, Liang C, Liu B, Tao Y and Tao H: Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways. Mol Med Rep 9: 345-349, 2014.
APA
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., & Tao, H. (2014). Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways. Molecular Medicine Reports, 9, 345-349. https://doi.org/10.3892/mmr.2013.1761
MLA
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., Tao, H."Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways". Molecular Medicine Reports 9.1 (2014): 345-349.
Chicago
Tao, L., Zhou, X., Shen, C., Liang, C., Liu, B., Tao, Y., Tao, H."Tetrandrine induces apoptosis and triggers a caspase cascade in U2-OS and MG-63 cells through the intrinsic and extrinsic pathways". Molecular Medicine Reports 9, no. 1 (2014): 345-349. https://doi.org/10.3892/mmr.2013.1761
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