Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-January Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-January Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Soluble fusion expression, characterization and localization of human β-defensin 6

  • Authors:
    • Aijie Xin
    • Yue Zhao
    • Heguo Yu
    • Huijuan Shi
    • Haiyan Liu
    • Hua Diao
    • Yonglian Zhang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China, Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, P.R. China, NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, P.R. China, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, P.R. China
  • Pages: 149-155
    |
    Published online on: October 31, 2013
       https://doi.org/10.3892/mmr.2013.1768
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Human β‑defensin 6 (DEFB106) is an antimicrobial peptide expressed in the epididymis, testis and lung, which indicates that DEFB106 may be involved in innate immunity and fertility. However, as a β‑defensin, this protein has not been well characterized. Using an intein‑mediated fusion expression system, the recombinant DEFB106 was expressed and purified (yield, 3‑5 mg/l) under optimized conditions. The purified protein was characterized using mass spectrometry and circular dichroism spectroscopy. The measured molecular weight was consistent with its theoretical value and the predominant secondary structure was β‑sheet, the common structure of β‑defensin family members. The purified DEFB106 showed antimicrobial activity against not only Escherichia coli (E. coli) and Candida albicans (C. albicans) SC5314, but also Staphylococcus aureus (S. aureus) CMCC26003. Furthermore, it exhibited a high affinity for heparin and lipopolysaccharide. In addition, it was determined that native DEFB106 was located in the epididymis, bone marrow and skin. These observations may aid in the determination of the physiological and pathological functions of DEFB106.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Thomma BP, Cammue BP and Thevissen K: Plant defensins. Planta. 216:193–202. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Lay FT and Anderson MA: Defensins - components of the innate immune system in plants. Curr Protein Pept Sci. 6:85–101. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Rodríguez de la Vega RC and Possani LD: On the evolution of invertebrate defensins. Trends Genet. 21:330–332. 2005.

4 

Lehrer RI and Ganz T: Defensins of vertebrate animals. Curr Opin Immunol. 14:96–102. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Hoffmann JA and Hetru C: Insect defensins: inducible antibacterial peptides. Immunol Today. 13:411–415. 1992. View Article : Google Scholar : PubMed/NCBI

6 

Tang YQ, Yuan J, Osapay G, et al: A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated alpha-defensins. Science. 286:498–502. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Yamaguchi Y, Nagase T, Makita R, et al: Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice. J Immunol. 169:2516–2523. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Kao CY, Chen Y, Zhao YH and Wu R: ORFeome-based search of airway epithelial cell-specific novel human β-defensin genes. Am J Respir Cell Mol Biol. 29:71–80. 2003.PubMed/NCBI

9 

Semple CA, Rolfe M and Dorin JR: Duplication and selection in the evolution of primate beta-defensin genes. Genome Biol. 4:R312003. View Article : Google Scholar : PubMed/NCBI

10 

Dubé E, Hermo L, Chan PT and Cyr DG: Alterations in gene expression in the caput epididymides of nonobstructive azoospermic men. Biol Reprod. 78:342–351. 2008.PubMed/NCBI

11 

Huang L, Ching CB, Jiang R and Leong SS: Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression. Protein Expr Purif. 61:168–174. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Diao H, Guo C, Lin D and Zhang Y: Intein-mediated expression is an effective approach in the study of beta-defensins. Biochem Biophys Res Commun. 357:840–846. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Dong J, Yu H, Zhang Y, Diao H and Lin D: Soluble fusion expression and characterization of human beta-defensin 3 using a novel approach. Protein Pept Lett. 18:1126–1132. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Yenugu S, Chintalgattu V, Wingard CJ, Radhakrishnan Y, French FS and Hall SH: Identification, cloning and functional characterization of novel beta-defensins in the rat (Rattus norvegicus). Reprod Biol Endocrinol. 4:72006. View Article : Google Scholar : PubMed/NCBI

15 

Abdiche Y, Malashock D, Pinkerton A and Pons J: Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet. Anal Biochem. 377:209–217. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Do T, Ho F, Heidecker B, Witte K, Chang L and Lerner L: A rapid method for determining dynamic binding capacity of resins for the purification of proteins. Protein Expr Purif. 60:147–150. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Mathys S, Evans TC, Chute IC, et al: Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene. 231:1–13. 1999. View Article : Google Scholar

18 

Schägger H and von Jagow G: Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 166:368–379. 1987.PubMed/NCBI

19 

Andersson E, Rydengård V, Sonesson A, Mörgelin M, Björck L and Schmidtchen A: Antimicrobial activities of heparin-binding peptides. Eur J Biochem. 271:1219–1226. 2004. View Article : Google Scholar : PubMed/NCBI

20 

McCullough BJ, Kalapothakis JM, Chin W, et al: Binding a heparin derived disaccharide to defensin inspired peptides: insights to antimicrobial inhibition from gas-phase measurements. Phys Chem Chem Phys. 12:3589–3596. 2010. View Article : Google Scholar

21 

Banki MR, Feng L and Wood DW: Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods. 2:659–661. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Guo C, Diao H, Lian Y, et al: Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E. J Biotechnol. 139:33–37. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Zhao Y, Diao H, Ni Z, Hu S, Yu H and Zhang Y: The epididymis-specific antimicrobial peptide β-defensin 15 is required for sperm motility and male fertility in the rat (Rattus norvegicus). Cell Mol Life Sci. 68:697–708. 2011.

24 

Pazgier M, Hoover DM, Yang D, Lu W and Lubkowski J: Human beta-defensins. Cell Mol Life Sci. 63:1294–1313. 2006. View Article : Google Scholar

25 

Liapina LA, Kondashevskaia MV, Kokriakov VN and Shamova OV: Interaction of heparin with defensin, a nonenzymatic cationic protein from neutrophils. Vopr Med Khim. 38:39–42. 1992.(In Russian).

26 

Seo ES, Blaum BS, Vargues T, et al: Interaction of human β-defensin 2 (HBD2) with glycosaminoglycans. Biochemistry. 49:10486–10495. 2010.

27 

Yang D, Chertov O, Bykovskaia SN, et al: Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science. 286:525–528. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Chen X, Niyonsaba F, Ushio H, et al: Antimicrobial peptides human beta-defensin (hBD)-3 and hBD-4 activate mast cells and increase skin vascular permeability. Eur J Immunol. 37:434–444. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Hoover DM, Boulegue C, Yang D, et al: The structure of human macrophage inflammatory protein-3alpha/CCL20. Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins. J Biol Chem. 277:37647–37654. 2002. View Article : Google Scholar

30 

Vargues T, Morrison GJ, Seo ES, et al: Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli. Protein Pept Lett. 16:668–676. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Ouellet M, Otis F, Voyer N and Auger M: Biophysical studies of the interactions between 14-mer and 21-mer model amphipathic peptides and membranes: insights on their modes of action. Biochim Biophys Acta. 1758:1235–1244. 2006. View Article : Google Scholar

32 

Shai Y: Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim Biophys Acta. 1462:55–70. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Buffy JJ, Hong T, Yamaguchi S, Waring AJ, Lehrer RI and Hong M: Solid-state NMR investigation of the depth of insertion of protegrin-1 in lipid bilayers using paramagnetic Mn2+. Biophys J. 85:2363–2373. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Motzkus D, Schulz-Maronde S, Heitland A, et al: The novel beta-defensin DEFB123 prevents lipopolysaccharide-mediated effects in vitro and in vivo. FASEB J. 20:1701–1702. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Liu H, Yu H, Gu Y, et al: Human beta-defensin DEFB126 is capable of inhibiting LPS-mediated inflammation. Appl Microbiol Biotechnol. 97:3395–3408. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Yu H, Dong J, Gu Y, Liu H, Xin A, et al: The novel human β-defensin 114 regulates lipopolysaccharide (LPS)-mediated inflammation and protects sperm from motility loss. J Biol Chem. 288:12270–12282. 2013.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xin A, Zhao Y, Yu H, Shi H, Liu H, Diao H and Zhang Y: Soluble fusion expression, characterization and localization of human β-defensin 6 . Mol Med Rep 9: 149-155, 2014.
APA
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., & Zhang, Y. (2014). Soluble fusion expression, characterization and localization of human β-defensin 6 . Molecular Medicine Reports, 9, 149-155. https://doi.org/10.3892/mmr.2013.1768
MLA
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., Zhang, Y."Soluble fusion expression, characterization and localization of human β-defensin 6 ". Molecular Medicine Reports 9.1 (2014): 149-155.
Chicago
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., Zhang, Y."Soluble fusion expression, characterization and localization of human β-defensin 6 ". Molecular Medicine Reports 9, no. 1 (2014): 149-155. https://doi.org/10.3892/mmr.2013.1768
Copy and paste a formatted citation
x
Spandidos Publications style
Xin A, Zhao Y, Yu H, Shi H, Liu H, Diao H and Zhang Y: Soluble fusion expression, characterization and localization of human β-defensin 6 . Mol Med Rep 9: 149-155, 2014.
APA
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., & Zhang, Y. (2014). Soluble fusion expression, characterization and localization of human β-defensin 6 . Molecular Medicine Reports, 9, 149-155. https://doi.org/10.3892/mmr.2013.1768
MLA
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., Zhang, Y."Soluble fusion expression, characterization and localization of human β-defensin 6 ". Molecular Medicine Reports 9.1 (2014): 149-155.
Chicago
Xin, A., Zhao, Y., Yu, H., Shi, H., Liu, H., Diao, H., Zhang, Y."Soluble fusion expression, characterization and localization of human β-defensin 6 ". Molecular Medicine Reports 9, no. 1 (2014): 149-155. https://doi.org/10.3892/mmr.2013.1768
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team