Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
2014-January Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-January Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Expression and significance of Wnt signaling components and their target genes in breast carcinoma

  • Authors:
    • Yanjiao He
    • Zhaoxia Liu
    • Chao Qiao
    • Miaosheng Xu
    • Jin Yu
    • Guang Li
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China
  • Pages: 137-143
    |
    Published online on: November 4, 2013
       https://doi.org/10.3892/mmr.2013.1774
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to investigate the expression of β-catenin, axin, cyclin D1 and c-myc, and their correlation with various clinicopathological factors of breast carcinoma. Using immunohistochemistry, the expression of axin, β-catenin, cyclin D1 and c-myc proteins was detected in 168 breast carcinomas and 40 normal breast tissue samples, as well as in 72 breast intraductal proliferative lesions. Correlations among the expression of these proteins with the clinicopathological factors of breast carcinomas were subsequently analyzed. Gene mutations of β-catenin (exon 3) in 44 cases of breast carcinoma were analyzed using polymerase chain reaction (PCR) followed by direct sequencing. In normal tissue, the epithelial cells demonstrated a marked membranous expression of β-catenin protein at cell-cell boundaries and positive axin expression; cyclin D1 and c-myc expression, however, were negative. The abnormal rate of β-catenin expression and the overexpression of cyclin D1 and c-myc were higher in breast carcinomas compared with breast cystic hyperplasia tissues. Positive axin expression levels were lower in breast carcinomas compared with breast intraductal proliferative lesions and normal breast tissues. Axin expression correlated inversely with tumor size, histological grade, clinical tumor, node, metastasis (TNM) stage and lymph node metastasis. The abnormal expression of β-catenin and the overexpression of cyclin D1 were correlated, and the overexpression of c-myc was correlated with tumor size, histological grade, clinical TNM stage and lymph node metastasis. The abnormal expression of β-catenin was correlated with the overexpression of cyclin D1, but not with the overexpression of c-myc. Lower levels of axin expression were correlated with higher levels of nuclear β-catenin expression. Mutations in the β-catenin gene were not detected in 44 cases of breast carcinoma. The abnormal expression of β-catenin may be key in the carcinogenesis and progression of human breast carcinoma by upregulating the expression of cyclin D1. The abnormal expression of β-catenin, the reduced expression of axin, and the overexpression of cyclin D1 and c-myc may be useful markers for determining metastasis, providing a prognosis for human breast carcinoma and for guiding treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Paul S and Dey A: Wnt signaling and cancer development: therapeutic implication. Neoplasma. 55:165–176. 2008.PubMed/NCBI

2 

Prosperi JR and Goss KH: A Wnt-ow of opportunity: targeting the Wnt/beta-catenin pathway in breast cancer. Curr Drug Targets. 11:1074–1088. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Miller JR: The Wnts. Genome Biol. 3:1–15. 2002.

4 

Polakis P: Wnt signaling and cancer. Genes Dev. 14:1837–1851. 2000.

5 

Lustig B and Behrens J: The Wnt signaling pathway and its role in tumor development. J Cancer Res Clin Oncol. 129:199–221. 2003.PubMed/NCBI

6 

Van Aken E, De Wever O, Correia da Rocha AS and Mareel M: Defective E-cadherin/catenin complexes in human cancer. Virchows Arch. 439:725–751. 2001.PubMed/NCBI

7 

Luo W and Lin SC: Axin: a master scaffold for multiple signaling pathways. Neurosignals. 13:99–113. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Böcker W: WHO classification of breast tumors and tumors of the female genital organs: pathology and genetics. Verh Dtsch Ges Pathol. 86:116–119. 2002.(In German).

9 

Maruyama K, Ochiai A, Akimoto S, et al: Cytoplasmic beta-catenin as a predictor of hematogenous metastasis in human colorectal cancer. Oncology. 59:302–309. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Nakajima M, Fukuchi M, Miyazaki T, et al: Reduced expression of Axin correlates with tumour progression of oesophageal squamous cell carcinoma. Br J Cancer. 88:1734–1739. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Bankfalvi A, Terpe HJ, Breukelmann D, et al: Immunophenotypic and prognostic analysis of E-cadherin and beta-catenin expression during breast carcinogenesis and tumour progression: a comparative study with CD44. Histopathology. 34:25–34. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Lin SY, Xia W, Wang JC, et al: Beta-catenin, a novel prognostic marker for breast cancer: its roles in cyclin D1 expression and cancer progression. Proc Natl Acad Sci USA. 97:4262–4266. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Lim SC and Lee MS: Significance of E-cadherin/beta-catenin complex and cyclin D1 in breast cancer. Oncol Rep. 9:915–928. 2002.PubMed/NCBI

14 

Dolled-Filhart M, McCabe A, Giltnane J, et al: Quantitative in situ analysis of beta-catenin expression in breast cancer shows decreased expression is associated with poor outcome. Cancer Res. 66:5487–5494. 2006. View Article : Google Scholar : PubMed/NCBI

15 

López-Knowles E, Zardawi SJ, McNeil CM, et al: Cytoplasmic localization of beta-catenin is a marker of poor outcome in breast cancer patients. Cancer Epidemiol Biomarkers Prev. 19:301–309. 2010.PubMed/NCBI

16 

Chung GG, Zerkowski MP, Ocal IT, et al: beta-Catenin and p53 analyses of a breast carcinoma tissue microarray. Cancer. 100:2084–2092. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Wang Z, Ren Y, He JJ, et al: Relationship between ectopic expression of Wnt/beta-catenin signaling pathway and Her-2 overexpression in human breast carcinoma. J Xi’an Jiaotong Univ (Med Sci). 30:587–591. 2009.

18 

Kikuchi A: Tumor formation by genetic mutations in the components of the Wnt signaling pathway. Cancer Sci. 94:225–229. 2003. View Article : Google Scholar : PubMed/NCBI

19 

He YJ, Jia XS, Qing SKZ, et al: The study on p53 and beta-catenin gene mutations in NK/T cell lymphoma. Chin J Clin Oncol. 31:1385–1388. 2004.

20 

Zhuang SM, Wiseman RW and Söderkvist P: Frequent mutations of the Trp53, Hras1 and beta-catenin (Catnb) genes in 1,3-butadiene-induced mammary adenocarcinomas in B6C3F1 mice. Oncogene. 21:5643–5648. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Howe LR and Brown AM: Wnt signaling and breast cancer. Cancer Biol Ther. 3:36–41. 2004. View Article : Google Scholar

22 

Ozaki S, Ikeda S, Ishizaki Y, et al: Alterations and correlations of the components in the Wnt signaling pathway and its target genes in breast cancer. Oncol Rep. 14:1437–1443. 2005.PubMed/NCBI

23 

Kizildag S, Zengel B, Vardar E, et al: beta-catenin gene mutation in invasive ductal breast cancer. J BUON. 13:533–536. 2008.PubMed/NCBI

24 

Abraham SC, Rcynolds C, Lee JH, et al: Fibromatosis of the breast and mutations involving the APC/beta-catenin pathway. Hum Pathol. 33:39–46. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Zeng L, Fagotto F, Zhang T, et al: The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell. 90:181–192. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Xu HT, Wang L, Lin D, et al: Abnormal beta-catenin and reduced axin expression are associated with poor differentiation and progression in non-small cell lung cancer. Am J Clin Pathol. 125:534–541. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Ishizaki Y, Ikeda S, Fujimori M, et al: Immunohistochemical analysis and mutational analyses of beta-catenin, Axin family and APC genes in hepatocellular carcinomas. Int J Oncol. 24:1077–1083. 2004.PubMed/NCBI

28 

Jin LH, Shao QJ, Luo W, et al: Detection of point mutations of the Axin1 gene in colorectal cancers. Int J Cancer. 107:696–699. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Cong F and Varmus H: Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of beta-catenin. Proc Natl Acad Sci USA. 101:2882–2887. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Cliffe A, Hamada F and Bienz M: A role of Dishevelled in relocating Axin to the plasma membrane during wingless signaling. Curr Biol. 13:960–966. 2003. View Article : Google Scholar

31 

Roh MS, Hong SH, Jeong JS, et al: Gene expression profiling of breast cancer with emphasis of beta-catenin regulation. J Korean Med Sci. 19:275–282. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
He Y, Liu Z, Qiao C, Xu M, Yu J and Li G: Expression and significance of Wnt signaling components and their target genes in breast carcinoma. Mol Med Rep 9: 137-143, 2014.
APA
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., & Li, G. (2014). Expression and significance of Wnt signaling components and their target genes in breast carcinoma. Molecular Medicine Reports, 9, 137-143. https://doi.org/10.3892/mmr.2013.1774
MLA
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., Li, G."Expression and significance of Wnt signaling components and their target genes in breast carcinoma". Molecular Medicine Reports 9.1 (2014): 137-143.
Chicago
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., Li, G."Expression and significance of Wnt signaling components and their target genes in breast carcinoma". Molecular Medicine Reports 9, no. 1 (2014): 137-143. https://doi.org/10.3892/mmr.2013.1774
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Liu Z, Qiao C, Xu M, Yu J and Li G: Expression and significance of Wnt signaling components and their target genes in breast carcinoma. Mol Med Rep 9: 137-143, 2014.
APA
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., & Li, G. (2014). Expression and significance of Wnt signaling components and their target genes in breast carcinoma. Molecular Medicine Reports, 9, 137-143. https://doi.org/10.3892/mmr.2013.1774
MLA
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., Li, G."Expression and significance of Wnt signaling components and their target genes in breast carcinoma". Molecular Medicine Reports 9.1 (2014): 137-143.
Chicago
He, Y., Liu, Z., Qiao, C., Xu, M., Yu, J., Li, G."Expression and significance of Wnt signaling components and their target genes in breast carcinoma". Molecular Medicine Reports 9, no. 1 (2014): 137-143. https://doi.org/10.3892/mmr.2013.1774
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team