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2014-January Volume 9 Issue 1

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Article

Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway

  • Authors:
    • Xiao-Fei Wang
    • Yi‑Bing Zhao
    • Qiang Wu
    • Zhi-Hua Sun
    • Hai-Jin Li
  • View Affiliations / Copyright

    Affiliations: Changzhou Maternal and Child Health Care Hospital of Nanjing Medical University, Changzhou 213003, P.R. China, Department of Gynecological Oncology, Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing 210009, P.R. China
  • Pages: 39-44
    |
    Published online on: November 8, 2013
       https://doi.org/10.3892/mmr.2013.1783
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Abstract

Triptolide (TP), the primary active component purified from the traditional Chinese herbal medicine Tripterygium wilfordii Hook. F (TWHF), has been shown to possess antitumor activity in several types of solid tumors. In the present study, we investigated the antitumor effect of TP in human endometrial cancer cells (HEC-1B) and elucidated its possible underlying mechanisms. HEC-1B cells were treated with various doses of TP (10, 20, 40, 80, 160 and 320 nM), and the cell viability was assessed by Cell Counting Kit-8 (CCK-8) and flow cytometric analysis. Results indicated that TP inhibited the proliferation of HEC-1B cells in a dose- and time‑dependent manner. To further investigate its mechanisms, the levels of apoptosis and the changes in caspase-3/9 expression in HEC-1B cells by pretreatment with z-VAD-fmk, a pan-caspase inhibitor, were detected by CCK-8 and western blotting. The cytotoxic effects of TP were significantly inhibited by z-VAD‑fmk. At the molecular level, TP did not effectively activate the p53 signaling pathway, but upregulated caspase-3/9 and downregulated bcl-2 without changing the bax level. Our studies revealed that TP has an effect on the apoptotic ability of endometrial cancer cells via a p53-independent mitochondrial pathway, presenting a novel strategy to evade drug resistance in tumorigenesis. The ability of TP to be a potential chemotherapeutic agent for endometrial cancer should be considered.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhao YB, Wu Q, Sun Z and Li H: Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway. Mol Med Rep 9: 39-44, 2014.
APA
Wang, X., Zhao, Y., Wu, Q., Sun, Z., & Li, H. (2014). Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway. Molecular Medicine Reports, 9, 39-44. https://doi.org/10.3892/mmr.2013.1783
MLA
Wang, X., Zhao, Y., Wu, Q., Sun, Z., Li, H."Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway". Molecular Medicine Reports 9.1 (2014): 39-44.
Chicago
Wang, X., Zhao, Y., Wu, Q., Sun, Z., Li, H."Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway". Molecular Medicine Reports 9, no. 1 (2014): 39-44. https://doi.org/10.3892/mmr.2013.1783
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhao YB, Wu Q, Sun Z and Li H: Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway. Mol Med Rep 9: 39-44, 2014.
APA
Wang, X., Zhao, Y., Wu, Q., Sun, Z., & Li, H. (2014). Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway. Molecular Medicine Reports, 9, 39-44. https://doi.org/10.3892/mmr.2013.1783
MLA
Wang, X., Zhao, Y., Wu, Q., Sun, Z., Li, H."Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway". Molecular Medicine Reports 9.1 (2014): 39-44.
Chicago
Wang, X., Zhao, Y., Wu, Q., Sun, Z., Li, H."Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway". Molecular Medicine Reports 9, no. 1 (2014): 39-44. https://doi.org/10.3892/mmr.2013.1783
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