Microarray‑based identification of nerve growth‑promoting genes in neurofibromatosis type I

Liu,Yufei; Kang,Dong; Li,Chunyan; Xu,Guang; Tan,Yan; Wang,Jinhong

doi:10.3892/mmr.2013.1785

2014-January Volume 9 Issue 1

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2014-January Volume 9 Issue 1

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Article

Microarray‑based identification of nerve growth‑promoting genes in neurofibromatosis type I

Authors:
- Yufei Liu
- Dong Kang
- Chunyan Li
- Guang Xu
- Yan Tan
- Jinhong Wang
View Affiliations / Copyright

Affiliations: Department of Pediatric Intensive Care Unit, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Huiqiao Department, South Hospital of Southern Medical University, Guangdong 510630, P.R. China, Department of Pediatric Respiratory Diseases, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Infectious Diseases, The First Affiliated Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Cancer Biotherapy Center, People's Hospital of Jilin Province, Changchun, Jilin 130000, P.R. China, Department of Respiratory, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510630, P.R. China
Pages: 192-196
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Published online on: November 8, 2013

https://doi.org/10.3892/mmr.2013.1785
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Abstract

As a genetic disease, neurofibromatosis type 1 (NF1) is characterized by abnormalities in multiple tissues derived from the neural crest, including neoplasms in the ends of the limbs. The exact mechanism of nerve growth in NF1 is unclear. In the present study, the gene expression profile of nerves in healthy controls and NF1 patients with macrodactylia of the fingers or toes were analyzed in order to identify possible genes associated with nerve growth. The Whole Human Genome Microarray was selected to screen for different gene expression profiles, and the result was analyzed with Feature Extraction software. The target genes were verified at the transcriptional and translational levels by reverse transcription‑polymerase chain reaction and western blotting, respectively. A common set of 28 genes were identified to be changed >5‑fold in the NF1 patients compared with the healthy controls. Among them, the metastasis‑associated genes, lymphocyte function‑associated antigen 1, C‑X‑C chemokine receptor type 4 and intracellular adhesion molecule 1, and the inflammatory cytokines, interleukin (IL) 1β, IL‑6 and IL‑8, were downregulated significantly. Mainly genes that changed >10‑fold were analyzed in this study, and HOXC8 demonstrated activity in promoting nerve growth. Through the analysis of the mRNA expression of the nerves in the NF1 patients, target molecules contributing to nerve growth during NF1 development were investigated, which aided in improving our understanding of this disease, and may provide a novel direction for nerve repair and regeneration.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Microarray‑based identification of nerve growth‑promoting genes in neurofibromatosis type I

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